S-nitrosoglutathione improves functional recovery in the isolated rat heart after cardioplegic ischemic arrest-evidence for a cardioprotective effect of nitric oxide.

The objective of this study was to assess the cardioprotective effect of the nitric oxide (.NO) donor, S-nitrosoglutathione (GSNO) and to investigate the mechanism of cardioprotection in a model of ischemia and reperfusion in isolated rat hearts. The role of .NO in myocardial protection was investigated by using nitronyl nitroxide as the .NO trap. Electron spin resonance spectroscopy was used to demonstrate that nitronyl nitroxide can trap .NO released from GSNO in a cardioplegic solution. .NO traps, oxyhemoglobin (4 mumol/l, n = 4) and nitronyl nitroxide (400 mumol/l, n = 5), inhibited the (2 mumol/l) GSNO-induced coronary vasodilation from the control value of 122% (n = 6) above base-line value to 73 and 60%, respectively. In the ischemia-reperfusion protocol, GSNO (20 mumol/l) was added to the cardioplegic solution during a 35-min ischemic arrest (n = 8). GSNO improved the functional recovery of ischemic hearts as compared to control (n = 6) as measured by the developed pressure (76 +/- 3 to 95 +/- 5% of base-line), rate pressure product (68 +/- 3 to 83 +/- 4% of base-line) and diastolic pressure (31 +/- 2 to 19 +/- 3 mm Hg). Reduction of coronary flow rate during reperfusion to control values in GSNO-treated hearts did not eliminate the improvement of functional recovery induced by GSNO. GSNO increased cyclic GMP production and slowed the accumulation of lactate (154 +/- 7 in control to 114 +/- 4 mumol/g dry wt.) and glucose-6-phosphate (3.66 +/- 0.19 in control to 2.18 +/- 0.10 mumol/g dry wt.) in myocardial tissue during ischemia.(ABSTRACT TRUNCATED AT 250 WORDS)

• Publication year

  1995

• Venue

  Journal of Pharmacology and Experimental Therapeutics

• Publication date

  1995-07-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0022-3565(25)10551-xPMID 7616400
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Nitronyl nitroxides as probes to study the mechanism of vasodilatory action of nitrovasodilators, nitrone spin traps, and nitroxides: role of nitric oxide.

  1995cited by this paper
• 8-bromo-cGMP reduces the myofilament response to Ca2+ in intact cardiac myocytes.

  1994cited by this paper
• Inhibition of hepatic gluconeogenesis by nitric oxide: a comparison with endotoxic shock.

  1994cited by this paper
• Mechanism of the diastolic dysfunction induced by glycolytic inhibition. Does adenosine triphosphate derived from glycolysis play a favored role in cellular Ca2+ homeostasis in ferret myocardium?

  1994cited by this paper
• Nitric oxide reacts with intracellular glutathione and activates the hexose monophosphate shunt in human neutrophils: evidence for S-nitrosoglutathione as a bioactive intermediary.

  1994cited by this paper
• Changes in myoplasmic sodium concentration during exposure to lactate in perfused rat heart.

  1994cited by this paper
• Cardiac preservation is enhanced in a heterotopic rat transplant model by supplementing the nitric oxide pathway.

  1994cited by this paper
• Nitric oxide prevents leukocyte adherence: role of superoxide.

  1993cited by this paper
• Control of cardiac muscle cell function by an endogenous nitric oxide signaling system.

  1993cited by this paper
• Inhibition of nitric oxide limits infarct size in the in situ rabbit heart.

  1993cited by this paper
• Transnitrosation as a predominant mechanism in the hypotensive effect of S-nitrosoglutathione.

  1993cited by this paper
• Nitric oxide regulates cardiac Ca2+ current. Involvement of cGMP-inhibited and cGMP-stimulated phosphodiesterases through guanylyl cyclase activation.

  1993cited by this paper
• Inhibition of nitric oxide production. Mechanisms of vascular albumin leakage.

  1993cited by this paper
• Antagonistic action of imidazolineoxyl N-oxides against endothelium-derived relaxing factor/.NO through a radical reaction.

  1993cited by this paper
• Nitric oxide attenuates cardiac myocyte contraction.

  1993cited by this paper
• Endothelial and Myocardial Cell Protection by a Cysteine‐Containing Nitric Oxide Donor After Myocardial Ischemia and Reperfusion

  1993cited by this paper
• Trapping of nitric oxide by nitronyl nitroxides: an electron spin resonance investigation.

  1993cited by this paper
• Inhibition by nitric oxide‐donors of human polymorphonuclear leucocyte functions

  1993cited by this paper
• Abnormal contractile function due to induction of nitric oxide synthesis in rat cardiac myocytes follows exposure to activated macrophage-conditioned medium.

  1993cited by this paper
• Lactate accumulation in isolated hypoxic rat ventricular myocardium: effect of different modulators of the cyclic GMP system.

  1993cited by this paper
• Biological activity of S-nitrosothiols: the role of nitric oxide.

  1993cited by this paper
• The Role of L‐Arginine in Ameliorating Reperfusion Injury After Myocardial Ischemia in the Cat

  1992cited by this paper
• Lack of tolerance to a 24-hour infusion of S-nitroso N-acetylpenicillamine (SNAP) in conscious rabbits.

  1992cited by this paper
• S‐nitroso‐glutathione inhibits platelet activation in vitro and in vivo

  1992cited by this paper
• Cardioprotection and attenuation of endothelial dysfunction by organic nitric oxide donors in myocardial ischemia-reperfusion.

  1992cited by this paper
• Reductive metabolism of nitroprusside in rat hepatocytes and human erythrocytes.

  1991cited by this paper
• cGMP inhibits the activity of single calcium channels in embryonic chick heart cells.

  1991cited by this paper
• Organic nitrate esters: clinical use and mechanisms of actions.

  1991cited by this paper
• Factors released from endocardium of the ferret and pig modulate myocardial contraction.

  1991cited by this paper
• Rapid tolerance to the hypotensive effects of glyceryl trinitrate in the rat: prevention by N‐acetyl‐l‐ but not N‐acetyl‐d‐cysteine

  1990cited by this paper
• Spontaneous liberation of nitric oxide cannot account for in vitro vascular relaxation by S-nitrosothiols.

  1990cited by this paper
• Effects of Diltiazem on Lactate, ATP, and Cytosolic Free Calcium Levels in Ischemic Hearts

  1990cited by this paper
• Ischemic preconditioning slows energy metabolism and delays ultrastructural damage during a sustained ischemic episode.

  1990cited by this paper
• Impaired Canine Coronary Vasodilator Response to Acetylcholine and Bradykinin After Occlusion‐ Reperfusion

  1989cited by this paper
• Role of Intracellular Na+ in Ca2+ Overload and Depressed Recovery of Ventricular Function of Reperfused Ischemic Rat Hearts Possible Involvement of H+-Na+ and Na+-Ca2+ Exchange

  1989cited by this paper
• On the Mechanism of NO Release from Sydnonimines

  1989cited by this paper
• Relationships between pre-ischemic ATP and glycogen content and post-ischemic recovery of rat heart.

  1988cited by this paper
• Correlation between nitric oxide formation during degradation of organic nitrates and activation of guanylate cyclase.

  1987cited by this paper
• Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor

  1987cited by this paper
• Myocardial ischemia revisited. The osmolar load, membrane damage, and reperfusion.

  1986cited by this paper
• Role of Glycolytic Products in Damage to Ischemic Myocardium: Dissociation of Adenosine Triphosphate Levels and Recovery of Function of Reperfused Ischemic Hearts

  1984cited by this paper
• Mechanism of vascular smooth muscle relaxation by organic nitrates, nitrites, nitroprusside and nitric oxide: evidence for the involvement of S-nitrosothiols as active intermediates.

  1981cited by this paper
• The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine

  1980cited by this paper
• Nitroprusside produces cyanide poisoning via reaction with hemoglobin.

  1974cited by this paper

• Inhaled Gases as Therapies for Post–Cardiac Arrest Syndrome: A Narrative Review of Recent Developments

  2021cites this paper
• Nitric oxide and the brain. Part 1: Mechanisms of regulation, transport and effects on the developing brain

  2020cites this paper
• Nitric Oxide-Releasing Polymeric Materials for Antimicrobial Applications: A Review

  2019cites this paper
• The renaissance of nitric oxide: from improvement of stability to enhancement of endocytosis

  2018cites this paper
• GSNO promotes functional recovery in experimental TBI by stabilizing HIF‐1&agr;

  2018cites this paper
• Cardioprotection by intermittent hypoxia conditioning: evidence, mechanisms, and therapeutic potential.

  2018cites this paper
• Amelioration of spinal cord injury in rats by blocking peroxynitrite/calpain activity

  2018cites this paper
• Regulation of IL-10 and IL-17 mediated experimental autoimmune encephalomyelitis by S-nitrosoglutathione.

  2018cites this paper
• Combined treatment with GSNO and CAPE accelerates functional recovery via additive antioxidant activities in a mouse model of TBI

  2018cites this paper
• Hypoxia inducible factor-1 alpha stabilization for regenerative therapy in traumatic brain injury

  2017cites this paper
• Can dendrimer based nanoparticles fight neurodegenerative diseases? Current situation versus other established approaches

  2017cites this paper
• Remote ischaemic conditioning and its effect on maladaptive cardiac remodelling following myocardial infarction

  2016cites this paper
• SNO promotes functional recovery in experimental TBI by stabilizing IF-1

  2016cites this paper
• The effect of thienopyridines and non-thienopyridines on nitric oxide metabolism in patients with stable angina

  2016cites this paper
• Promoting endothelial function by S-nitrosoglutathione through the HIF-1α/VEGF pathway stimulates neurorepair and functional recovery following experimental stroke in rats

  2015cites this paper
• An NO/GSNO-based Neuroregeneration Strategy for Stroke Therapy

  2015cites this paper
• Benefits of Nitric Oxide Cues to Matrix Synthesis by Healthy and Aneurysmal Human Smooth Muscle Cells within 3D Cocultures

  2014cites this paper
• and 8-Hour Storage Cardioprotection by Activation of NO/cGMP Pathway After Cardioplegic Arrest

  2013cites this paper
• Discriminative EPR detection of NO and HNO by encapsulated nitronyl nitroxides

  2013cites this paper
• Protective Role of S-Nitrosoglutathione (GSNO) Against Cognitive Impairment in Rat Model of Chronic Cerebral Hypoperfusion

  2013cites this paper
• Stimulation of functional recovery via the mechanisms of neurorepair by S-nitrosoglutathione and motor exercise in a rat model of transient cerebral ischemia and reperfusion

  2012cites this paper
• S-Nitrosoglutathione reduces oxidative injury and promotes mechanisms of neurorepair following traumatic brain injury in rats

  2011cites this paper
• Changes in the nitric oxide level in the rat liver as a response to brain injury.

  2011cites this paper
• Reduced ischemia/reperfusion injury via glutathione-initiated nitric oxide-releasing dendrimers.

  2010cites this paper
• Nitric Oxide Plays a Key Role in Myelination in the Developing Brain

  2010cites this paper
• Mitochondria as metabolizers and targets of nitrite.

  2010cites this paper
• Effects of dinitrosyl iron complex with glutathione and its components on ischemic rat heart during reperfusion

  2009cites this paper
• [Effects of dinitrosyl iron complex on metabolism and cellular membranes in ischemic rat heart].

  2009cites this paper
• Therapeutic strategies by modulating oxygen stress in cancer and inflammation.

  2009cites this paper
• Neuroprotective strategies in parkinson’s disease: protection against progressive nigral damage induced by free radicals

  2009cites this paper
• Glutathione Metabolism: Favorable Versus Unfavorable Effects

  2008cites this paper
• Oxidants in biology: a question of balance

  2008cites this paper
• S-nitrosothiol-modified dendrimers as nitric oxide delivery vehicles.

  2008cites this paper
• Mitochondria as a Target for the Cardioprotective Effects of Nitric Oxide in Ischemia–Reperfusion Injury

  2008cites this paper
• Nitric oxide induces heat shock protein 72 production and delayed protection against myocardial ischemia in rabbits via activating protein kinase C.

  2008cites this paper
• Cardioprotection & mitochondrial S-nitrosation: effects of S-nitroso-2-mercaptopropionyl glycine (SNO-MPG) in Cardiac ischemia-reperfusion injury

  2007cites this paper
• Synthesis and characterization of dendrimer-based nitric oxide delivery systems

  2007cites this paper
• Role of S-nitrosothiol transport in the cardioprotective effects of S-nitrosocysteine in rat hearts.

  2007cites this paper
• Mitochondrial contribution to the molecular mechanism of heart acclimatization to chronic hypoxia: role of nitric oxide.

  2007cites this paper
• Cardioprotection and mitochondrial S-nitrosation: effects of S-nitroso-2-mercaptopropionyl glycine (SNO-MPG) in cardiac ischemia-reperfusion injury.

  2007cites this paper
• Preconditioning Results in S-Nitrosylation of Proteins Involved in Regulation of Mitochondrial Energetics and Calcium Transport

  2007cites this paper
• Binding of polyaminocarboxylate chelators to the active-site copper inhibits the GSNO-reductase activity but not the superoxide dismutase activity of Cu,Zn-superoxide dismutase.

  2006cites this paper
• Nitric oxide modulates the expression of matricellular genes involved in fibrosis in renal glomerular mesangial cells

  2005cites this paper
• The effect of nitric oxide on glucose metabolism

  2004cites this paper
• Nitric oxide in myocardial ischemia/reperfusion injury.

  2004cites this paper
• Oxidative Stress and Adaptation of the Infant Heart to Hypoxia and Ischemia

  2004cites this paper
• THE EFFECT OF S-NITROSOGLUTATHIONE ON INSULIN RECEPTOR BEHAVIOR ON ERYTHROCYTES IN AN ANIMAL MODEL

  2004cites this paper
• Gene expression profiles in children undergoing cardiac surgery for right heart obstructive lesions.

  2004cites this paper
• Electron paramagnetic resonance for quantitation of nitric oxide in aqueous solutions.

  2002cites this paper
• Chronic Hypoxia Increases Endothelial Nitric Oxide Synthase Generation of Nitric Oxide by Increasing Heat Shock Protein 90 Association and Serine Phosphorylation

  2002cites this paper
• Reactions of S-nitrosothiols with L-ascorbic acid in aqueous solution.

  2002cites this paper
• Bioimaging of nitric oxide.

  2002cites this paper
• Astrocytes protect neurons from nitric oxide toxicity by a glutathione‐dependent mechanism

  2001cites this paper
• Metabolism of S-nitrosoglutathione by endothelial cells.

  2001cites this paper
• Cardioprotective function of inducible nitric oxide synthase and role of nitric oxide in myocardial ischemia and preconditioning: an overview of a decade of research.

  2001cites this paper
• Nitric oxide activates the sarcolemmal K(ATP) channel in normoxic and chronically hypoxic hearts by a cyclic GMP-dependent mechanism.

  2001cites this paper
• Detection and Imaging of Endogenously Produced Nitric Oxide with Electron Paramagnetic Resonance Spectroscopy

  2000cites this paper
• Reaction of S-Nitrosoglutathione with the Heme Group of Deoxyhemoglobin*

  2000cites this paper
• Glutathione protects against myocardial ischemia-reperfusion injury by detoxifying peroxynitrite.

  2000cites this paper
• Role of nitric oxide and superoxide in acute cardiac allograft rejection in rats.

  2000cites this paper
• Biological chemistry and clinical potential of S-nitrosothiols.

  2000cites this paper
• Kinetics and mechanism of the decomposition of S-nitrosoglutathione by l-ascorbic acid and copper ions in aqueous solution to produce nitric oxide.

  2000cites this paper
• Mechanisms of biological S-nitrosation and its measurement

  2000cites this paper
• Protective Effect of S-Nitrosylated α1-Protease Inhibitor on Hepatic Ischemia-Reperfusion Injury

  2000cites this paper
• Modification of creatine kinase by S-nitrosothiols: S-nitrosation vs. S-thiolation.

  2000cites this paper
• Capturing the sublimity of a free radical gas.

  1999cites this paper
• Neuroprotective Properties of Nitric Oxide

  1999cites this paper
• Nitrosothiol Formation Catalyzed by Ceruloplasmin

  1999cites this paper
• The redox pathway of S-nitrosoglutathione, glutathione and nitric oxide in cell to neuron communications.

  1999cites this paper
• The peroxynitrite generator, SIN-1, becomes a nitric oxide donor in the presence of electron acceptors.

  1999cites this paper
• Adaptation to Chronic Hypoxia Confers Tolerance to Subsequent Myocardial Ischemia by Increased Nitric Oxide Production

  1999cites this paper
• Mechanism of superoxide dismutase/H(2)O(2)-mediated nitric oxide release from S-nitrosoglutathione--role of glutamate.

  1999cites this paper
• Depletion of Intracellular Glutathione Increases Susceptibility to Nitric Oxide in Mesencephalic Dopaminergic Neurons

  1999cites this paper
• Free Radicals in Disease

  1998cites this paper
• Effect of sodium nitroprusside and L-arginine methyl ester on rat hearts stored at 4 degrees C for 24 h.

  1998cites this paper
• Thiols protect the inhibition of myocardial aconitase by peroxynitrite.

  1998cites this paper
• Effect of sodium nitroprusside and L-arginine methyl ester on rat hearts stored at 4 °C for 24 h

  1998cites this paper
• Adaptation of hearts to chronic hypoxia increases tolerance to subsequent ischemia by increased nitric oxide production.

  1998cites this paper
• Neuroprotection by S‐nitrosoglutathione of brain dopamine neurons from oxidative stress

  1998cites this paper
• Cardioprotection by activation of NO/cGMP pathway after cardioplegic arrest and 8-hour storage.

  1998cites this paper
• Immunopharmacologic agents in the amelioration of hepatic injuries.

  1998cites this paper
• S-Nitrosoglutathione as a substrate for gamma-glutamyl transpeptidase.

  1997cites this paper
• An assay method for nitric oxide-related compounds in whole blood.

  1997cites this paper
• Possible dual role of nitric oxide in oxidative stress injury: a study in perfused hepatocytes.

  1997cites this paper
• Effects of nitroprusside as a nitric oxide donor on anoxia/reoxygenation and D-galactosamine hepatic injuries: a study in perfused hepatocytes.

  1997cites this paper
• Mechanism of Nitric Oxide Release from S-Nitrosothiols*

  1996cites this paper
• S‐Nitrosoglutathione induces formation of nitrosylmyoglobin in isolated hearts during cardioplegic ischemia — an electron spin resonance study

  1996cites this paper
• The mechanism of cardioprotection by S‐nitrosoglutathione monoethyl ester in rat isolated heart during cardioplegic ischaemic arrest

  1996cites this paper
• Detection of nitric oxide by electron spin resonance in chemical, photochemical, cellular, physiological, and pathophysiological systems.

  1996cites this paper
• Interaction of nitric oxide with photoexcited rose bengal: evidence for one-electron reduction of nitric oxide to nitroxyl anion.

  1995cites this paper