K-ras Mutational Status in Cytohistological Tissue as a Molecular Marker for the Diagnosis of Pancreatic Cancer: A Systematic Review and Meta-Analysis

Background. More clinically meaningful diagnostic tests are needed in pancreatic cancer (PC). K-ras mutations are the most frequently acquired genetic alteration. Methods. Original research articles involving the diagnostic accuracy of K-ras mutation detection in PC were selected. Data were presented as forest plots and summary receiver operating characteristic (SROC) curve analysis was used to summarize the overall test performance. Results. We assessed 19 studies from 16 published articles. The reports were divided into three groups according to the process used to obtain the test material. The summary estimates for detecting K-ras status using an invasive method (fine needle aspiration (FNA), endoscopic retrograde cholangiopancreatography (ERCP), or surgery) were better than cytology: the pooled sensitivity was 77% (95% confidence interval (CI): 74–80%) versus 54% (95% CI: 47–61%); specificity was 88% (95% CI: 85–91%) versus 91% (95% CI: 83–96%); and diagnostic odds ratio (DOR) was 20.26 (11.40–36.03) versus 7.52 (95% CI: 2.80–20.18), respectively. When two procedures were combined, the diagnostic accuracy was markedly improved. Conclusions. The analysis of K-ras mutations in pancreatic tissue has a promising diagnostic significance in PC. Further valuable studies are needed.

• Publication year

  2014

• Venue

  Disease Markers

• Publication date

  2014-07-16

• Fields of study

  Medicine

• Identifiers
  DOI 10.1155/2014/573783PMID 25301978PMCID 4124783
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Circulating MicroRNAs as a Novel Class of Diagnostic Biomarkers in Gastrointestinal Tumors Detection: A Meta-Analysis Based on 42 Articles

  2014cited by this paper
• Pancreatic cancer.

  2013cited by this paper
• Combination Therapy of Ursodeoxycholic Acid and Corticosteroids for Primary Biliary Cirrhosis with Features of Autoimmune Hepatitis: A Meta-Analysis

  2013cited by this paper
• KRAS mutation does not predict the efficacy of neo-adjuvant chemoradiotherapy in rectal cancer: a systematic review and meta-analysis.

  2013cited by this paper
• Clinical impact of K-ras mutation analysis in EUS-guided FNA specimens from pancreatic masses.

  2012cited by this paper
• [Pancreatic carcinoma].

  2012cited by this paper
• Clinical impact of K-ras mutation analysis in EUS-guided FNA specimens from pancreatic masses

  2012cited by this paper
• Clinical validity of detecting K-ras mutations for the diagnosis of exocrine pancreatic cancer: a prospective study in a clinically-relevant spectrum of patients

  2011cited by this paper
• QUADAS-2: A Revised Tool for the Quality Assessment of Diagnostic Accuracy Studies

  2011cited by this paper
• Cigarette smoking and K-ras mutations in pancreas, lung and colorectal adenocarcinomas: etiopathogenic similarities, differences and paradoxes.

  2009cited by this paper
• Molecular markers of pancreatic cancer: development and clinical relevance

  2008cited by this paper
• Oncogenes and cancer.

  2008cited by this paper
• Sensitive and quantitative detection of KRAS2 gene mutations in pancreatic duct juice differentiates patients with pancreatic cancer from chronic pancreatitis, potential for early detection

  2008cited by this paper
• Bmc Medical Research Methodology Open Access Intra-cluster Correlation Coefficients in Adults with Diabetes in Primary Care Practices: the Vermont Diabetes Information System Field Survey

  2006cited by this paper
• Molecular pathogenesis of pancreatic adenocarcinoma: potential clinical implications.

  2006cited by this paper
• Mimicks of pancreatic malignancy in patients with chronic pancreatitis: correlation of computed tomography imaging features with histopathologic findings.

  2006cited by this paper
• Clinical significance of k-ras and c-erbB-2 mutations in pancreatic adenocarcinoma and chronic pancreatitis

  2005cited by this paper
• Molecular markers of early pancreatic cancer.

  2005cited by this paper
• Pancreatic cancer

  2004cited by this paper
• K-ras gene mutation in the diagnosis of ultrasound guided fine-needle biopsy of pancreatic masses.

  2003cited by this paper
• Detection of K-ras gene mutation at codon 12 by pancreatic duct brushing for pancreatic cancer.

  2003cited by this paper
• K-ras gene mutation in the diagnosis of ultrasound guided fine-needle biopsy of pancreatic masses

  2003cited by this paper
• Quantitative analysis of K-ras gene mutation in pancreatic tissue obtained by endoscopic ultrasonography-guided fine needle aspiration: clinical utility for diagnosis of pancreatic tumor

  2002cited by this paper
• Genetic diagnosis of pancreatic cancer.

  2002cited by this paper
• Prognostic Implications of Routine, Immunohistochemical, and Molecular Staging in Resectable Pancreatic Adenocarcinoma

  2002cited by this paper
• Properties of the summary receiver operating characteristic (SROC) curve for diagnostic test data

  2002cited by this paper
• Early detection of pancreatic carcinoma.

  2002cited by this paper
• A Prospective Study of Detection of Pancreatic Carcinoma by Combined Plasma K -ras Mutations and Serum CA19-9 Analysis

  2002cited by this paper
• Quantity of mutant K-ras gene in pancreatic secretions for diagnosis of pancreatic carcinoma with different assays: analysis of 100 patients.

  2002cited by this paper
• Quantitative analysis of K-ras gene mutation in pancreatic tissue obtained by endoscopic ultrasonography-guided fine needle aspiration: clinical utility for diagnosis of pancreatic tumor.

  2002cited by this paper
• Systematic reviews in health care: Systematic reviews of evaluations of diagnostic and screening tests.

  2001cited by this paper
• Approaches to heterogeneity in meta‐analysis

  2001cited by this paper
• Email submissions from outside the United Kingdom

  2001cited by this paper
• Detection of K-ras gene mutations in plasma DNA of patients with pancreatic adenocarcinoma : Correlation with clinicopathological features

  2001cited by this paper
• K‐ras oncogene subtype mutations are associated with survival but not expression of p53, p16INK4A, p21WAF‐1, cyclin D1, erbB‐2 and erbB‐3 in resected pancreatic ductal adenocarcinoma

  2000cited by this paper
• Significance of K-ras mutation and CEA level in pancreatic juice in the diagnosis of pancreatic cancer.

  2000cited by this paper
• Progression model for pancreatic cancer.

  2000cited by this paper
• K‐ras mutations in duodenal aspirate without secretin stimulation for screening of pancreatic and biliary tract carcinoma

  1999cited by this paper
• High proportion of mutant K-ras gene in pancreatic juice of patients with pancreatic cystic lesions

  1999cited by this paper
• K-ras mutations in DNA extracted from the plasma of patients with pancreatic carcinoma: diagnostic utility and prognostic significance.

  1999cited by this paper
• EUS and K-ras analysis of pure pancreatic juice collected via a duodenoscope after secretin stimulation for diagnosis of pancreatic mass lesion: a prospective study.

  1999cited by this paper
• K-ras mutations in tissue and stool samples from patients with pancreatic cancer and chronic pancreatitis

  1999cited by this paper
• K-ras gene mutations in the diagnosis of fine-needle aspirates of pancreatic masses: prospective study using two techniques with different detection limits.

  1998cited by this paper
• Detection of K-ras gene mutations in plasma DNA of patients with pancreatic adenocarcinoma: correlation with clinicopathological features.

  1998cited by this paper
• Quantitative Determination of K‐ras Mutations in Pancreatic Juice for Diagnosis of Pancreatic Cancer Using Hybridization Protection Assay

  1998cited by this paper
• Ki‐ras mutations in exocrine pancreatic cancer: Association with clinico‐pathological characteristics and with tobacco and alcohol consumption

  1997cited by this paper
• Tumor-suppressive pathways in pancreatic carcinoma.

  1997cited by this paper
• Clinical and Epidemiologic Features of Primary HIV Infection

  1996cited by this paper
• Diagnosis of pancreatic adenocarcinoma by polymerase chain reaction from pancreatic secretions.

  1994cited by this paper
• Pancreatic carcinoma.

  1992cited by this paper
• p53 mutations in human cancers.

  1991cited by this paper
• The c‐erbB‐2 proto‐oncogene in human pancreatic cancer

  1990cited by this paper
• Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes.

  1988cited by this paper
• Oncogenes and cancer.

  1983cited by this paper
• Bmc Medical Research Methodology Open Access a Proposed Architecture and Method of Operation for Improving the Protection of Privacy and Confidentiality in Disease Registers

  year unknowncited by this paper

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  2023cites this paper
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  2023cites this paper
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  2022cites this paper
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  2022cites this paper
• RAS Mutations in Human Cancers: Roles in Precision Medicine.

  2019cites this paper
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  2018cites this paper
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  2018cites this paper
• A quarter century of EUS-FNA: Progress, milestones, and future directions

  2018cites this paper
• Therapeutic Endoscopy in the Management of Pancreatic Cancer

  2016cites this paper
• Telomerase activity in pancreatic juice differentiates pancreatic cancer from chronic pancreatitis: A meta-analysis.

  2016cites this paper
• Microarray expression profile analysis of long non-coding RNAs in pancreatic ductal adenocarcinoma.

  2016cites this paper
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  2016cites this paper