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Label-free Quantifications of Multiplexed Mycotoxins by G-quadruplex based on Photonic Barcodes.

Dagan Zhang,Lijun Cai,Feika Bian,Tiantian Kong,Yuanjin Zhao

Published 2020 in Analytical Chemistry

ABSTRACT

Multiplexed quantification of mycotoxins is of great significance in food safety. Here, a novel photonic crys-tal (PhC) barcodes with G-quadruplex aptamer encapsu-lated for label-free multiplex mycotoxins quantification are developed. The probes are immobilized on PhC bar-codes to form molecular beacon (MB), which contain the sequences of mycotoxins aptamers and G-quadruplex. In the presence of target, the hairpin struc-ture of MB would open and the region of G-quadruplex is exposed, which subsequently combines with Thiofla-vin T (ThT) to produce fluorescence. The relative fluo-rescence intensity increased as the mycotoxins concen-tration increased in a linear range from 1.0 pg/mL to 100 ng/mL. Moreover, the multiplexed mycotoxins quantification could be achieved by tuning the structural color of the PhC barcodes. We demonstrate that this method with high accuracy and specificity for multi-plexed detection of mycotoxins, with the sensitivity of the detection as low as 0.70 pg/mL. Our results show that G-quadruplex-encapsulated PhC barcodes offer a novel simple and label-free pathway toward the multi-plex screen assay of mycotoxins for food safety.

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