Spatially Controlled Supramolecular Polymerization of Peptide Nanotubes by Microfluidics.

The recent advances in the supramolecular polymerization of synthetic building blocks in aqueous conditions has given rise to new artificial and biocompatible functional materials. However, despite the importance of spatially resolved self-assembly for natural and artificial molecular machines, the spatial control of supramolecular polymerization with synthetic monomers has not been experimentally established yet. Here we describe a microfluidic-regulated tandem process of supramolecular polymerization and droplet encapsulation to control the position of self-assembled microfibrillar bundles of cyclic peptide nanotubes in water droplets. This method allowed the precise preferential localization of the fibres either at the interface or into the core of the droplets. UV absorbance, circular dichroism and fluorescence microscopy indicated that the microfluidic control of the stimuli (changes in pH or ionic strength) can be employed to adjust the packing degree and the spatial position of microfibrillar bundles of cyclic peptide nanotubes. Additionally, this spatially organized supramolecular polymerization of peptide nanotubes was applied in the assembly of highly ordered two-dimensional droplet networks.

• Publication year

  2020

• Venue

  Angewandte Chemie

• Publication date

  2020-02-04

• Fields of study

  Medicine, Materials Science, Chemistry, Engineering

• Identifiers
  DOI 10.1002/anie.202000103PMID 32017352
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Faculty Opinions recommendation of Supramolecular caging for cytosolic delivery of anionic probes.

  2020cited by this paper
• Supramolecular caging for cytosolic delivery of anionic probes

  2019cited by this paper
• 1D to 2D Self Assembly of Cyclic Peptides.

  2019cited by this paper
• Multivalent Ultrasensitive Interfacing of Supramolecular 1D Nanoplatforms

  2019cited by this paper
• Supramolecular assembly of functional peptide-polymer conjugates.

  2019cited by this paper
• How Defects Control the Out-of-Equilibrium Dissipative Evolution of a Supramolecular Tubule.

  2019cited by this paper
• Self-assembled micro-fibres by oxime connection of linear peptide amphiphiles.

  2019cited by this paper
• Growing Prospects of Dynamic Covalent Chemistry in Delivery Applications.

  2019cited by this paper
• Chirality Effects on Peptide Self-Assembly Unraveled from Molecules to Materials

  2018cited by this paper
• Supplementary Information for Chemically activating MoS 2 via spontaneous atomic palladium interfacial doping towards efficient hydrogen evolution

  2018cited by this paper
• Macromolecularly Crowded Protocells from Reversibly Shrinking Monodisperse Liposomes

  2018cited by this paper
• Dissipative adaptation in driven self-assembly leading to self-dividing fibrils

  2018cited by this paper
• Towards Functional Droplet Architectures: a Belousov-Zhabotinsky Medium for Networks

  2018cited by this paper
• Hydrogel and Organogel Formation by Hierarchical Self-Assembly of Cyclic Peptides Nanotubes.

  2018cited by this paper
• Oscillations, travelling fronts and patterns in a supramolecular system

  2018cited by this paper
• Communication and quorum sensing in non-living mimics of eukaryotic cells

  2018cited by this paper
• Probing the Dynamic Nature of Self‐Assembling Cyclic Peptide–Polymer Nanotubes in Solution and in Mammalian Cells

  2018cited by this paper
• Patterned Arrays of Supramolecular Microcapsules

  2018cited by this paper
• pH-Triggered self-assembly and hydrogelation of cyclic peptide nanotubes confined in water micro-droplets.

  2018cited by this paper
• Engineering Microstructure with Evaporation‐Induced Self‐Assembly of Microdroplets

  2018cited by this paper
• Synthetic materials at the forefront of gene delivery

  2018cited by this paper
• Supramolecular functional assemblies: dynamic membrane transporters and peptide nanotubular composites.

  2017cited by this paper
• Molecular photoswitches mediating the strain-driven disassembly of supramolecular tubules

  2017cited by this paper
• How the Internet of cells has biologists buzzing

  2017cited by this paper
• Recent advances in controlling the internal and external properties of self-assembling cyclic peptide nanotubes and dimers.

  2017cited by this paper
• Supplementary Information for Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics

  2017cited by this paper
• Drug delivery by supramolecular design.

  2017cited by this paper
• DNA cytoskeleton for stabilizing artificial cells

  2017cited by this paper
• Interplay between Ions, the Cytoskeleton, and Cell Wall Properties during Tip Growth1[OPEN]

  2017cited by this paper
• Cucurbit[n]uril-Based Microcapsules Self-Assembled within Microfluidic Droplets: A Versatile Approach for Supramolecular Architectures and Materials

  2017cited by this paper
• Microfluidic Assembly of Monodisperse Vesosomes as Artificial Cell Models.

  2017cited by this paper
• Introduction to Applied Colloid and Surface Chemistry

  2016cited by this paper
• Supramolecular Polymers in Aqueous Media.

  2016cited by this paper
• Milliseconds Make the Difference in the Far-from-Equilibrium Self-Assembly of Supramolecular Chiral Nanostructures.

  2016cited by this paper
• Catalysis of Supramolecular Hydrogelation.

  2016cited by this paper
• Controlling molecular transport in minimal emulsions

  2016cited by this paper
• Shape control of lipid bilayer membranes by confined actin bundles.

  2015cited by this paper
• Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro

  2015cited by this paper
• Applications of Supramolecular Anion Recognition.

  2015cited by this paper
• Self-Assembly of Silver Metal Clusters of Small Atomicity on Cyclic Peptide Nanotubes.

  2015cited by this paper
• Coupling of carbon and peptide nanotubes.

  2014cited by this paper
• Cascade reactions in multicompartmentalized polymersomes.

  2014cited by this paper
• Topology and dynamics of active nematic vesicles

  2014cited by this paper
• Self-organization of the bacterial cell-division protein FtsZ in confined environments

  2013cited by this paper
• A Tissue-Like Printed Material

  2013cited by this paper
• Peptide-based microcapsules obtained by self-assembly and microfluidics as controlled environments for cell culture

  2013cited by this paper
• Ion channel models based on self-assembling cyclic peptide nanotubes.

  2013cited by this paper
• Assembly of MreB filaments on liposome membranes: a synthetic biology approach.

  2012cited by this paper
• One-Step Fabrication of Supramolecular Microcapsules from Microfluidic Droplets

  2012cited by this paper
• Directed cytoskeleton self-organization.

  2012cited by this paper
• Cytoskeletal Protein Expression and its Association within the Hydrophobic Membrane of Artificial Cell Models

  2012cited by this paper
• Cytoskeletal-like supramolecular assembly and nanoparticle-based motors in a model protocell.

  2011cited by this paper
• Electrostatics at the nanoscale.

  2011cited by this paper
• Self-organized patterns of actin filaments in cell-sized confinement

  2011cited by this paper
• Molecular mechanism of Thioflavin-T binding to amyloid fibrils.

  2010cited by this paper
• Biocompatible surfactants for water-in-fluorocarbon emulsions.

  2008cited by this paper
• Designer emulsions using microfluidics

  2008cited by this paper
• Intermolecular Forces in the Self‐Assembly of Peptide Amphiphile Nanofibers

  2006cited by this paper
• On the organization of self-assembled actin networks in giant vesicles

  2003cited by this paper
• Microtubules, membranes and cytokinesis.

  2000cited by this paper
• Self-assembling organic nanotubes based on a cyclic peptide architecture

  1993cited by this paper

• 3D self-assembly of cyclic peptides into multilayered nanosheets.

  2026cites this paper
• Self-assembled nanotubes from the supramolecular polymerization of discrete cyclic entities.

  2025cites this paper
• Highly Efficient Construction and in Situ Post-Assembly Modification of Multifunctional Metal-Organic Cages in Microdroplets.

  2025cites this paper
• 2D Assemblies Based on a Tetraphenylethylene d,l‐Cyclic Peptide Scaffold

  2025cites this paper
• Concentration-Dependent Nanosheet and Nanotube Networks: Ice-Water Interface Control for Antifreeze Application.

  2025cites this paper
• Heavy-Atom-Free Supramolecular Photosensitizers Derived from Conventional Fluorophores.

  2025cites this paper
• Protocol for photo-controlling the assembly of cyclic peptide nanotubes in solution and inside microfluidic droplets

  2024cites this paper
• Designer peptide–DNA cytoskeletons regulate the function of synthetic cells

  2024cites this paper
• Supramolecular fibrillation in coacervates and other confined systems towards biomimetic function

  2024cites this paper
• Microfluidic-Assisted Self-Assembly of Molecular Hydrogelator at Water-Water Interfaces for Continuous Fabrication of Supramolecular Microcapsules.

  2024cites this paper
• Stimuli-Triggered Dynamic Transformations in Supramolecular Polymers

  2024cites this paper
• DNA-amphiphilic nanostructures: synthesis, characterization and applications.

  2024cites this paper
• Cascading oxidoreductases-like nanozymes for high selective and sensitive fluorescent detection of ascorbic acid

  2024cites this paper
• Charge controlled interactions between DNA-modified silica nanoparticles and fluorosurfactants in microfluidic water-in-oil droplets

  2023cites this paper
• Photo-assembling cyclic peptides for dynamic light-driven peptide nanotubes

  2023cites this paper
• Dynamic responsiveness of self‐assembling peptide‐based nano‐drug systems

  2023cites this paper
• Highly Efficient Self-Assembly of Metallacages and Their Supramolecular Catalysis Behaviors in Microdroplets.

  2023cites this paper
• Recent Progress on Cyclic Peptides’ Assembly and Biomedical Applications

  2023cites this paper
• Self-assembly of cyclic peptide monolayers by hydrophobic supramolecular hinges

  2023cites this paper
• Self-healing cyclic peptide hydrogels.

  2022cites this paper
• Cyclization and Self-Assembly of Cyclic Peptides.

  2022cites this paper
• Bottom-up supramolecular assembly in two dimensions

  2022cites this paper
• Membrane-Bound Inward-Growth of Artificial Cytoskeletons and Their Selective Disassembly.

  2022cites this paper
• Constructing dual-functional porphyrin-based thorium metal-organic framework toward photocatalytic uranium(VI) reduction integrated with organic oxidation

  2022cites this paper
• Microfluidic-driven ultrafast self-assembly of a dipeptide into stimuli-responsive 0D, 1D, and 2D nanostructures and as hydrolase mimic.

  2022cites this paper
• Hierarchical Materials from High Information Content Macromolecular Building Blocks: Construction, Dynamic Interventions, and Prediction.

  2022cites this paper
• Nanoscale Assembly of Functional Peptides with Divergent Programming Elements

  2021cites this paper
• Hierarchical self-assembly of aromatic peptide conjugates into supramolecular polymers: it takes two to tango

  2021cites this paper
• Enabling Technology for Supramolecular Chemistry

  2021cites this paper
• Supramolecular fibrillation of peptide amphiphiles induces environmental responses in aqueous droplets

  2021cites this paper
• Hybrid Self‐Assembled Gel Beads for Tuneable pH‐Controlled Rosuvastatin Delivery

  2021cites this paper
• Molecular Plumbing to Bend Self‐Assembling Peptide Nanotubes

  2021cites this paper
• Self-assembled low-molecular-weight gelator injectable microgel beads for delivery of bioactive agents

  2021cites this paper
• Microfluidic enhancement of self-assembly systems.

  2021cites this paper
• Molecular Self-Assembly and Supramolecular Chemistry of Cyclic Peptides

  2021cites this paper
• Smart Nanocages as a Tool for Controlling Supramolecular Aggregation.

  2021cites this paper
• Supramolecular Dipeptide-Based Near-Infrared Fluorescent Nanotubes for Cellular Mitochondria Targeted Imaging and Early Apoptosis.

  2021cites this paper
• Synthetic Supramolecular Systems in Life-like Materials and Protocell Models

  2020cites this paper
• A minimalistic catalytically-active cell mimetic made of a supra-molecular hydrogel encapsulated into a polymersome.

  2020cites this paper
• Sequence Decoding of 1D to 2D Self-Assembling Cyclic Peptides.

  2020cites this paper