Cupin Variants as Macromolecular Ligand Library for Stereoselective Michael Addition of Nitroalkanes.

Cupin superfamily proteins (TM1459) work as a macromolecular ligand framework with a double-stranded β-barrel structure ligating to a Cu ion through histidine side chains. Variegating the first coordination sphere of TM1459 revealed that H52A and H54A/H58A mutants effectively catalyzed the diastereo- and enantio-selective Michael addition reaction of nitroalkanes to an α,β-unsaturated ketone. Moreover, in silico substrate docking signified C106N and F104W single-point mutations, which inverted the diastereoselectivity of H52A and further improved the stereoselectivity of H54A/H58A, respectively.

• Publication year

  2019

• Venue

  Angewandte Chemie

• Publication date

  2019-12-31

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.26434/chemrxiv.11481834PMID 32073197
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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