Somatic Liver Knockout (SLiK): A Quick and Efficient Way to Generate Liver‐Specific Knockout Mice Using Multiplex CRISPR/Cas9 Gene Editing

Somatic liver knockout (SLiK) is a method developed to rapidly generate a liver‐specific knockout of one or several genes. This technique combines the strengths of CRISPR/Cas9 gene editing and hydrodynamic tail‐vein injection, a simple in vivo method for transfection of hepatocytes, to harness the powerful selection pressure of tyrosinemic livers to replace host hepatocytes with any desired gene deletion. In this protocol, we will describe sgRNA design and cloning, hydrodynamic tail‐vein injection of targeting constructs, and screening and validation methods for efficient in vivo gene editing. © 2020 by John Wiley & Sons, Inc.

• Publication year

  2020

• Venue

  Current Protocols in Molecular Biology

• Publication date

  2020-03-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1002/cpmb.117PMID 32150344PMCID PMC7500866
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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