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Genetically-enriched microbe-facilitated self-healing nano-concrete

B. Chattopadhyay

Published 2020 in Unknown venue

ABSTRACT

Abstract Growing trends in the development of self-healing property of cementitious material has prepared several smart materials with versatile properties and high sustainability. Self-healing phenomenon in construction material will prolong the service life of infrastructures. Recently, attempts are being made to establish autonomous-healing activity by incorporating mineral producing microbes in the concrete mix. But the efficient self-healing phenomenon through the process of bacteria assisted bio-mineralization occurs at the initial stage of concretization. Self-healing on the cracks within the concrete that appear over a prolonged period will be the most desirable and challenging task that scientists are trying to establish. An alkaliphilic spore forming Bacillus strain is used for obtaining true self-healing attribute in concrete. Researchers have established that the gene of bioremediase-like protein (isolated from BKH2) when transformed into the spore forming bacterium Bacillus subtilis, the bioremediase like protein is expressed in the transformed bacterial cells and the transformed bacterium acts as a true self-healing agent in cementitious materials. Nano-rod like Gehlenite crystals due to the action of bioremediase like protein and calcite crystals due to the action of urase gene of Bacillus subtilis synergistically repair the cracks and fill the pores of the cementitious matrix for which the genetically transformed Bacillus subtilis bacterium incorporated cementitious material behave like a true self-healing material.

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