Cell type-specific gene expression in the Drosophila melanogaster male accessory gland.

The accessory gland of the male Drosophila melanogaster plays a vital role in reproduction. This secretory organ synthesizes products that are transferred to the female and are necessary to elicit the proper physiological and behavioral responses in the female. The accessory gland is composed of two morphologically distinct secretory cell types, the main cells and the secondary cells. Previous studies identified some genes expressed in main cells or in all accessory gland cells. In this paper we use P-element mediated enhancer traps to examine gene expression in the accessory gland. We show that, in addition to genes expressed in main cells only or in all accessory gland secretory cells, there are genes expressed specifically in secondary cells. Each cell type is uniform in the expression of its genes. Our results demonstrate that the two cell types are not only morphologically distinct but also biochemically distinct. We also show that the two cell types differ in their regulation of gene expression in response to mating activity.

• Publication year

  1992

• Venue

  Mechanisms of Development

• Publication date

  1992-07-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/0925-4773(92)90036-JPMID 1525037
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A clonal analysis of cell lineage and growth in the male and female genital disc ofDrosophila melanogaster

  2004cited by this paper
• Cytogenic mapping and isolation of mutations of the β-Gal-1 locus of Drosophila melanogaster

  2004cited by this paper
• Ectopic expression of sex peptide alters reproductive behavior of female D. melanogaster.

  1991cited by this paper
• Structure, cell-specific expression, and mating-induced regulation of a Drosophila melanogaster male accessory gland gene.

  1990cited by this paper
• Synthesis of two Drosophila male accessory gland proteins and their fate after transfer to the female during mating.

  1990cited by this paper
• Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector.

  1989cited by this paper
• P-element-mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes in Drosophila.

  1989cited by this paper
• P-element-mediated enhancer detection: a versatile method to study development in Drosophila.

  1989cited by this paper
• A male accessory gland peptide that regulates reproductive behavior of female D. melanogaster.

  1988cited by this paper
• Determination of male-specific gene expression in Drosophila accessory glands.

  1988cited by this paper
• Structure and expression of a Drosophila male accessory gland gene whose product resembles a peptide pheromone precursor.

  1988cited by this paper
• Juvenile hormone action mediated in male accessory glands of Drosophila by calcium and kinase C.

  1988cited by this paper
• Sequences expressed sex-specifically in Drosophila melanogaster adults.

  1987cited by this paper
• Detection in situ of genomic regulatory elements in Drosophila.

  1987cited by this paper
• Isolation of the eclosion gene cluster and the developmental expression of the Gld gene in Drosophila melanogaster.

  1986cited by this paper
• Spatial and temporal pattern of hsp26 expression during normal development.

  1986cited by this paper
• Protein metabolism of Drosophila melanogaster male accessory glands—III: Stimulation of protein synthesis following copulation

  1985cited by this paper
• The preferential translation of Drosophila hsp70 mRNA requires sequences in the untranslated leader.

  1985cited by this paper
• Studies of esterase 6 in Drosophila melanogaster—XVI: Synthesis occurs in the male reproductive tract (anterior ejaculatory duct) and is modulated by juvenile hormone

  1984cited by this paper
• THE FUNCTIONAL MORPHOLOGY AND BIOCHEMISTRY OF INSECT MALE ACCESSORY GLANDS AND THEIR SECRETIONS

  1984cited by this paper
• New heat shock puffs and β=galactosidase activity resulting from transformation of Drosophila with an hsp70-lacZ hybrid gene

  1983cited by this paper
• Cytodiffrentiation in the accessory glands of Tenebrio molitor. VI. A congruent map of cells and their secretions in the layered elastic product of the male bean‐shaped gland

  1980cited by this paper
• The Role of Male Accessory Glands in Insect Reproduction

  1976cited by this paper
• The Genetics and Biology of Drosophila

  1976cited by this paper
• Some Aspects of the Reproductive Biology of Drosophila: Sperm Transfer, Sperm Storage, and Sperm Utilization

  1973cited by this paper
• The control of sexual receptivity in female Drosophila.

  1962cited by this paper

• Single-nucleus resolution of sex-biased expression and dosage compensation in Drosophila melanogaster.

  2026cites this paper
• Oncogenic signaling in the Drosophila prostate-like accessory gland activates a pro-tumorigenic program in the absence of proliferation

  2025cites this paper
• Unusual Occurrence of Syncytial Epithelia in the Male Accessory Glands of Shore Bugs (Leptopodomorpha in Hemiptera)

  2025cites this paper
• Oncogenic signaling in the adult Drosophila prostate-like accessory gland leads to activation of a conserved pro-tumorigenic program, in the absence of proliferation

  2024cites this paper
• Unusual modes of cell and nuclear divisions characterise Drosophila development

  2024cites this paper
• Rapid promoter evolution of male accessory gland genes is accompanied by divergent expression in closely related Drosophila species

  2024cites this paper
• Post-eclosion growth in the Drosophila Ejaculatory Duct is driven by Juvenile Hormone signaling and is essential for male fertility

  2024cites this paper
• Dynamics of seminal fluid production after mating

  2024cites this paper
• Cell cycle variants during Drosophila male accessory gland development

  2024cites this paper
• Transcriptional programs are activated and microRNAs are repressed within minutes after mating in the Drosophila melanogaster female reproductive tract

  2023cites this paper
• Aging Fly Cell Atlas Identifies Exhaustive Aging Features at Cellular Resolution

  2023cites this paper
• Cytoophidia safeguard binucleation of Drosophila male accessory gland cells

  2022cites this paper
• Evolution of secondary cell number and position in the Drosophila accessory gland

  2022cites this paper
• Identification of a micropeptide and multiple secondary cell genes that modulate Drosophila male reproductive success

  2021cites this paper
• Female copulation song is modulated by seminal fluid

  2020cites this paper
• Endocycling in the adult Drosophila accessory gland

  2019cites this paper
• Cell cycle variants during Drosophila accessory gland development

  2019cites this paper
• Transcriptional regulation and functions of the Drosophila paired gene

  2019cites this paper
• Modulation of seminal fluid molecules by males and females.

  2019cites this paper
• Endocycles support tissue growth and regeneration of the adult Drosophila accessory gland

  2019cites this paper
• The impact of ageing on male reproductive success in Drosophila melanogaster

  2018influential citation
• Dynamic changes in ejaculatory bulb size during Drosophila melanogaster aging and mating.

  2018cites this paper
• Genetic regulation of membrane trafficking, exosome secretion, growth and dense-core granule biogenesis in the Drosophila accessory gland

  2018influential citation
• Anatomy and Histology of the Male Reproductive Complex of the Onion Maggot Fly, Delia Antiqua, (Diptera: Anthomyiidae) Including Some Comparisons With D. Platura and D. Radicum

  2018cites this paper
• Rab‐mediated trafficking in the secondary cells of Drosophila male accessory glands and its role in fecundity

  2018influential citation
• The effect of mating history on male reproductive ageing in Drosophila melanogaster.

  2018cites this paper
• Binucleation of Accessory Gland Lobe Contributes to Effective Ejection of Seminal Fluid in Drosophila melanogaster

  2018cites this paper
• Control of seminal fluid protein expression via regulatory hubs in Drosophila melanogaster

  2018cites this paper
• The lncRNA male-specific abdominal plays a critical role in Drosophila accessory gland development and male fertility

  2018cites this paper
• The Drosophila Accessory Gland as a Model for Prostate Cancer and Other Pathologies.

  2017cites this paper
• Gene expression changes in male accessory glands during ageing are accompanied by reproductive decline in Drosophila melanogaster

  2017influential citation
• Seminal Plasma Plays Important Roles in Fertility

  2017cites this paper
• The sperm cell : production, maturation, fertilization, regeneration

  2017cites this paper
• Functional male accessory glands and fertility in Drosophila require novel ecdysone receptor

  2017cites this paper
• Chapter Eleven: The Drosophila Accessory Gland as a Model for Prostate Cancer and Other Pathologies

  2017cites this paper
• The Female Post-Mating Response Requires Genes Expressed in the Secondary Cells of the Male Accessory Gland in Drosophila melanogaster

  2016cites this paper
• Nature and Functions of Glands and Ducts in the Drosophila Reproductive Tract

  2016cites this paper
• Early reproductive success in Drosophila males is dependent on maturity of the accessory gland

  2016cites this paper
• Induction of Excessive Endoplasmic Reticulum Stress in the Drosophila Male Accessory Gland Results in Infertility

  2015cites this paper
• Loss of flfl Triggers JNK-Dependent Cell Death in Drosophila

  2015cites this paper
• Male Age Affects Female Mate Preference, Quantity of Accessory Gland Proteins, and Sperm Traits and Female Fitness in D. melanogaster

  2015cites this paper
• Genetic Interactions between the Members of the SMN-Gemins Complex in Drosophila

  2015cites this paper
• The RED domain of Paired is specifically required for Drosophila accessory gland maturation

  2015cites this paper
• BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior

  2014cites this paper
• Synthesis, depletion and cell-type expression of a protein from the male accessory glands of the dengue vector mosquito Aedes aegypti.

  2014cites this paper
• A genetic screen in Drosophila for regulators of human prostate cancer progression.

  2014cites this paper
• Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophila male accessory gland cells

  2014cites this paper
• A Novel Function for the Hox Gene Abd-B in the Male Accessory Gland Regulates the Long-Term Female Post-Mating Response in Drosophila

  2013cites this paper
• The Homeodomain Protein Defective Proventriculus Is Essential for Male Accessory Gland Development to Enhance Fecundity in Drosophila

  2012cites this paper
• Oh, the places they’ll go

  2012cites this paper
• Bone morphogenetic protein- and mating-dependent secretory cell growth and migration in the Drosophila accessory gland

  2012cites this paper
• Binucleation of Drosophila Adult Male Accessory Gland Cells Increases Plasticity of Organ Size for Effective Reproduction

  2012cites this paper
• Regulation of adult physiology and behavior in Drosophila melanogaster

  2012cites this paper
• REGULATION OF ADULT PHYSIOLOGY AND BEHAVIOR

  2012cites this paper
• Multinucleated smooth muscles and mononucleated as well as multinucleated striated muscles develop during establishment of the male reproductive organs of Drosophila melanogaster.

  2012cites this paper
• Report Seminal Fluid Protein Allocation and Male Reproductive Success

  2009cites this paper
• Ultrastructure of male reproductive accessory glands and ejaculatory duct in the Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae).

  2009cites this paper
• Hazardous effect of tannery solid waste leachates on development and reproduction in Drosophila melanogaster: 70kDa heat shock protein as a marker of cellular damage.

  2009cites this paper
• Seminal fluid protein allocation and male reproductive success.

  2009cites this paper
• Seminal fluid protein depletion and replenishment in the fruit fly, Drosophila melanogaster: an ELISA-based method for tracking individual ejaculates

  2009cites this paper
• Mechanisms underlying sexual selection and sexual conflict in Drosophila melanogaster.

  2007cites this paper
• Adverse effect of organophosphate compounds, dichlorvos and chlorpyrifos in the reproductive tissues of transgenic Drosophila melanogaster: 70kDa heat shock protein as a marker of cellular damage.

  2007cites this paper
• Seminal influences: Drosophila Acps and the molecular interplay between males and females during reproduction.

  2007cites this paper
• Natural variation in male-induced ‘cost-of-mating’ and allele-specific association with male reproductive genes in Drosophila melanogaster

  2006cites this paper
• Identification of genes expressed in the accessory glands of male Mediterranean Fruit Flies (Ceratitis capitata).

  2006cites this paper
• Sperm Competitive Ability in Drosophila melanogaster Associated With Variation in Male Reproductive Proteins

  2005cites this paper
• GAL4 enhancer trap targeting of the Drosophila sex determination gene fruitless

  2005cites this paper
• Gradual release of sperm bound sex-peptide controls female postmating behavior in Drosophila.

  2005cites this paper
• 1.5 – Gonadal Glands and Their Gene Products

  2005cites this paper
• A genomic analysis of Drosophila somatic sexual differentiation and its regulation

  2004cites this paper
• Functions and analysis of the seminal fluid proteins of male Drosophila melanogaster fruit flies.

  2004cites this paper
• Summary A genomic analysis of Drosophila somatic sexual differentiation and its regulation

  2004cites this paper
• Ultrastructure of the male reproductive accessory glands in the medfly Ceratitis capitata (Diptera: Tephritidae) and preliminary characterization of their secretions.

  2003cites this paper
• Dual role of the Pax gene paired in accessory gland development of Drosophila.

  2002cites this paper
• The gifts that keep on giving: physiological functions and evolutionary dynamics of male seminal proteins in Drosophila

  2002cites this paper
• Male Accessory Gland Secretory Proteins in nasuta Subgroup of Drosophila: Synthetic Activity of Acp

  2002cites this paper
• The Drosophila melanogaster X-linked mfs(1)6E locus is required for production of normal seminal fluid by the male accessory glands.

  2001cites this paper
• Evolutionary EST analysis identifies rapidly evolving male reproductive proteins in Drosophila

  2001cites this paper
• Regulation of Mst57Dc Expression in Male Accessory Glands of Dorsophila melanogaster

  2000cites this paper
• Drosophila seminal fluid proteins enter the circulatory system of the mated female fly by crossing the posterior vaginal wall.

  1999cites this paper
• A 45-kDa cAMP-dependent phosphoprotein which is related to the product of Mst57Dc in Drosophila melanogaster.

  1999cites this paper
• Seminal fluid regulation of female sexual attractiveness in Drosophila melanogaster.

  1998cites this paper
• Tokens of love: functions and regulation of Drosophila male accessory gland products.

  1997cites this paper
• Mating and hormonal triggers regulate accessory gland gene expression in male Drosophila.

  1997cites this paper
• New genes for male accessory gland proteins in Drosophila melanogaster.

  1997cites this paper
• Functional domains are specified to single-cell resolution in a Drosophila epithelium.

  1997cites this paper
• Mapping domains of gene expression in the Malpighian tubule of Drosophila melanogaster by enhancer trapping

  1996cites this paper
• The accessory gland proteins in maleDrosophila: structural, reproductive, and evolutionary aspects

  1996cites this paper
• Localization of the Drosophila male accessory gland protein Acp36DE in the mated female suggests a role in sperm storage.

  1996cites this paper
• The Drosophila Sex-Peptide: A Peptide Pheromone Involved in Reproduction

  1996cites this paper
• Structure and regulation of a gene cluster for male accessory gland transcripts in Drosophila melanogaster.

  1995cites this paper
• Cost of mating in Drosophila melanogaster females is mediated by male accessory gland products

  1995cites this paper
• A Drosophila male accessory gland protein that is a member of the serpin superfamily of proteinase inhibitors is transferred to females during mating.

  1995cites this paper
• Two tightly-linked Drosophila male accessory gland transcripts with the same developmental expression derive from independent transcription units.

  1994cites this paper
• A Sec62p‐related component of the secretory protein translocon from Drosophila displays developmentally complex behavior.

  1994cites this paper
• The regulation of sex determination and sexually dimorphic differentiation in Drosophila.

  1993cites this paper
• Probing the function of Drosophila melanogaster accessory glands by directed cell ablation.

  1993cites this paper
• The sex-peptide.

  1992cites this paper
• Dynamics of seminal fluid replenishment after mating

  year unknowncites this paper