An Integrated Microarray Analysis Reveals Significant Diagnostic and Prognostic Biomarkers in Pancreatic Cancer

Background Pancreatic cancer (PAC) is a lethal cancer and it is essential to develop accurate diagnostic and prognostic biomarkers for PAC. Material/Methods An integrated microarray analysis of PAC was conducted to identify differentially expressed genes (DEGs) between PAC and non-tumor controls. Expression of DEGs were further confirmed by The Cancer Genome Atlas and the Genotype-Tissue Expression. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis, and protein–protein integration network construction were performed to further research the biological functions of DEGs. Receiver-operating characteristic analysis and survival analysis were used to evaluate the diagnostic and prognostic value of DEGs for PAC. Results Seventeen microarray datasets were downloaded from Gene Expression Omnibus to conduct the integrated microarray analysis. A total of 1136 DEGs (596 upregulated and 540 downregulated DEGs) in PAC tissues compared with non-tumor controls were identified. Pancreatic secretion (Kegg: 04972), insulin signaling pathway (Kegg: 04910), and several cancer-related pathways including pathways in cancer (Kegg: 05200), MAPK signaling pathway (Kegg: 04010), and pancreatic cancer (Kegg: 05212) were enriched for DEGs in PAC. Seven DEGs (AHNAK2, CDH3, IFI27, ITGA2, LAMB3, SLC6A14, and TMPRSS4) were found to have both great diagnostic and prognostic value for PAC. High expression of these 7 DEGs were significantly associated with poor prognosis of patients with PAC. Conclusions These 7 DEGs might be potential diagnostic and prognostic biomarkers for PAC and help uncovering the mechanism of PAC.

• Publication year

  2020

• Venue

  Medical Science Monitor

• Publication date

  2020-02-08

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.12659/MSM.921769PMID 32235821PMCID 7148424
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cancer statistics, 2019

  2019cited by this paper
• Interferon α-inducible protein 27 is an oncogene and highly expressed in cholangiocarcinoma patients with poor survival

  2019cited by this paper
• An Integrative Data Mining and Omics-Based Translational Model for the Identification and Validation of Oncogenic Biomarkers of Pancreatic Cancer

  2019cited by this paper
• LAMB3 mediates apoptotic, proliferative, invasive, and metastatic behaviors in pancreatic cancer by regulating the PI3K/Akt signaling pathway

  2019cited by this paper
• LAMB3 is associated with disease progression and cisplatin cytotoxic sensitivity in head and neck squamous cell carcinoma.

  2019cited by this paper
• Overexpression of BUB1B, CCNA2, CDC20, and CDK1 in tumor tissues predicts poor survival in pancreatic ductal adenocarcinoma

  2019cited by this paper
• The root of Actinidia chinensis inhibits hepatocellular carcinomas cells through LAMB3

  2018cited by this paper
• An integrated methylation and gene expression microarray analysis reveals significant prognostic biomarkers in oral squamous cell carcinoma

  2018cited by this paper
• LINC01121 Inhibits Cell Apoptosis While Facilitating Proliferation, Migration, and Invasion Though Negative Regulation of the Camp/PKA Signaling Pathway via GLP1R

  2018cited by this paper
• Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression.

  2018cited by this paper
• Knockdown of IFI27 inhibits cell proliferation and invasion in oral squamous cell carcinoma

  2018cited by this paper
• Identification and Validation of a Diagnostic and Prognostic Multi-Gene Biomarker Panel for Pancreatic Ductal Adenocarcinoma

  2018cited by this paper
• Identification of Prognostic Biomarkers by Combined mRNA and miRNA Expression Microarray Analysis in Pancreatic Cancer

  2018cited by this paper
• Analysis of dynamic molecular networks for pancreatic ductal adenocarcinoma progression

  2018cited by this paper
• Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries

  2018cited by this paper
• Splice variants as novel targets in pancreatic ductal adenocarcinoma

  2017cited by this paper
• AHNAK2 is a potential prognostic biomarker in patients with PDAC

  2017influential reference
• Upregulated LAMB3 increases proliferation and metastasis in thyroid cancer

  2017cited by this paper
• Prognostic relevance of molecular subtypes and master regulators in pancreatic ductal adenocarcinoma

  2016cited by this paper
• Globally increased ultraconserved noncoding RNA expression in pancreatic adenocarcinoma

  2016cited by this paper
• Meta-analysis of transcriptome data identifies a novel 5-gene pancreatic adenocarcinoma classifier

  2016cited by this paper
• Amino acid transporter SLC6A14 is a novel and effective drug target for pancreatic cancer

  2016cited by this paper
• A Novel MIF Signaling Pathway Drives the Malignant Character of Pancreatic Cancer by Targeting NR3C2.

  2016cited by this paper
• Transcriptomic and Immunohistochemical Profiling of SLC6A14 in Pancreatic Ductal Adenocarcinoma

  2015cited by this paper
• IP-10/CXCL10 induction in human pancreatic cancer stroma influences lymphocytes recruitment and correlates with poor survival

  2014cited by this paper
• TMPRSS4 correlates with colorectal cancer pathological stage and regulates cell proliferation and self-renewal ability

  2014cited by this paper
• TMPRSS4 regulates levels of integrin α5 in NSCLC through miR-205 activity to promote metastasis

  2014cited by this paper
• Characterization of gene expression and activated signaling pathways in solid-pseudopapillary neoplasm of pancreas

  2014cited by this paper
• TMPRSS4 upregulates uPA gene expression through JNK signaling activation to induce cancer cell invasion.

  2014cited by this paper
• Generation and Characterisation of Novel Pancreatic Adenocarcinoma Xenograft Models and Corresponding Primary Cell Lines

  2014cited by this paper
• TMPRSS4: an emerging potential therapeutic target in cancer

  2014cited by this paper
• A multi-gene signature predicts outcome in patients with pancreatic ductal adenocarcinoma

  2014cited by this paper
• MicroRNAs cooperatively inhibit a network of tumor suppressor genes to promote pancreatic tumor growth and progression.

  2014cited by this paper
• Membrane Proteins Involved in Epithelial-Mesenchymal Transition and Tumor Invasion: Studies on TMPRSS4 and TM4SF5

  2014cited by this paper
• Integration of Metabolomics and Transcriptomics Revealed a Fatty Acid Network Exerting Growth Inhibitory Effects in Human Pancreatic Cancer

  2013cited by this paper
• The role of micro RNAs let7c, 100 and 218 expression and their target RAS, C-MYC, BUB1, RB, SMARCA5, LAMB3 and Ki-67 in prostate cancer

  2013cited by this paper
• The Genotype-Tissue Expression (GTEx) project

  2013cited by this paper
• TMPRSS4 as a Poor Prognostic Factor for Triple-Negative Breast Cancer

  2013cited by this paper
• Integrative Survival-Based Molecular Profiling of Human Pancreatic Cancer

  2012cited by this paper
• Overexpression of TMPRSS4 in non-small cell lung cancer is associated with poor prognosis in patients with squamous histology

  2011cited by this paper
• TMPRSS4 induces invasion and epithelial-mesenchymal transition through upregulation of integrin alpha5 and its signaling pathways.

  2010cited by this paper
• Moderated effect size and P-value combinations for microarray meta-analyses

  2009cited by this paper
• Identification of a Novel Tumor-Associated Antigen, Cadherin 3/P-Cadherin, as a Possible Target for Immunotherapy of Pancreatic, Gastric, and Colorectal Cancers

  2008cited by this paper
• Combined gene expression analysis of whole-tissue and microdissected pancreatic ductal adenocarcinoma identifies genes specifically overexpressed in tumor epithelia.

  2008cited by this paper
• The AHNAKs are a class of giant propeller-like proteins that associate with calcium channel proteins of cardiomyocytes and other cells

  2004cited by this paper
• Interferon alpha-inducible protein 27 (IFI27) is upregulated in psoriatic skin and certain epithelial cancers.

  2004cited by this paper
• A novel transmembrane serine protease (TMPRSS3) overexpressed in pancreatic cancer.

  2000cited by this paper

• Exploration of the correlation between AHNAK2 and pancreatic cancer and its role in the tumor microenvironment based on bioinformatics: Computational pharmacology

  2025cites this paper
• Computational theranostics strategy for pancreatic ductal adenocarcinoma.

  2025cites this paper
• Construction and validation of a diagnostic model for dermatomyositis based on the LASSO algorithm

  2025cites this paper
• AHNAK2 Promotes the Progression of Pancreatic Ductal Adenocarcinoma by Maintaining the Stability of c-MET

  2024cites this paper
• Identification of New Potential Targets for Pancreatic Ductal Adenocarcinoma by Integrated Bioinformatic Analysis

  2024cites this paper
• LAMB3: Central role and clinical significance in neoplastic and non-neoplastic diseases.

  2024cites this paper
• Multi-omics analysis of LAMB3 as a potential immunological and biomarker in pan-cancer

  2023cites this paper
• Genetics, Genomics and Emerging Molecular Therapies of Pancreatic Cancer

  2023cites this paper
• Identification of MIRNA-MRNA-HUB Gene Network Prediction in Squamous Cell Lung Carcinoma Via Starbase Server and Insilico Analysis of Hub Genes

  2023cites this paper
• Proteomics-Driven Biomarkers in Pancreatic Cancer

  2023cites this paper
• Identification of Biomarkers in Key Gene Prediction in Lung Carcinoma

  2023cites this paper
• AHNAKs roles in physiology and malignant tumors

  2023cites this paper
• The Obscure Potential of AHNAK2

  2022cites this paper
• NREP is a Diagnostic and Prognostic Biomarker, and Promotes Gastric Cancer Cell Proliferation and Angiogenesis

  2022cites this paper
• Value in Survival and Therapeutic Response for Patients with Lung Adenocarcinoma

  2022cites this paper
• Construction of a Redox-Related Prognostic Model with Predictive Value in Survival and Therapeutic Response for Patients with Lung Adenocarcinoma

  2022cites this paper
• AHNAK2 is a biomarker and a potential therapeutic target of adenocarcinomas

  2022cites this paper
• ITGA2, LAMB3, and LAMC2 may be the potential therapeutic targets in pancreatic ductal adenocarcinoma: an integrated bioinformatics analysis

  2021cites this paper
• AHNAK2 is a novel prognostic marker and correlates with immune infiltration in papillary thyroid cancer: Evidence from integrated analysis.

  2020cites this paper
• Amino Acid Transporter SLC6A14 (ATB0,+) – A Target in Combined Anti-cancer Therapy

  2020cites this paper