The effect of conocarpan on susceptibility of Candida albicans to phagocytosis and digestion by macrophages

Piper solmsianum C. DC. compounds exhibit several properties, including antimicrobial activity. The aim of the present study was to investigate whether conocarpan alters Candida albicans growth or killing of the yeast by macrophages. Conocarpan showed strong activity against the yeast with minimal inhibitory concentration (MIC) of 20 µg/mL and minimal fungicidal concentration (MFC) of 30 µg/mL. Mice peritoneal cells (macrophages) were cultured for 24 and 48 hours in supplemented RPMI 1640 medium. Cellular activation was assessed by determining MTT reduction and nitric oxide production. Standardized tests were conducted to select the optimal parameters for the subsequent killing test. Results showed that conocarpan exhibited antifungal activity and that C. albicans cultivated in the presence of the compound had greater susceptibility to death by macrophages. These findings suggest that conocarpan may have potential as an antimicrobial agent for C. albicans infections, promoting macrophagic immune support by altering growth of the yeast.

• Publication year

  2019

• Venue

  Brazilian Journal of Biological Sciences

• Publication date

  2019-12-31

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.21472/bjbs.061403
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Neutrophil function: from mechanisms to disease.

  2012cited by this paper
• Interplay between Candida albicans and the Mammalian Innate Host Defense

  2012cited by this paper
• Lignan Derivatives from Krameria lappacea Roots Inhibit Acute Inflammation in Vivo and Pro-inflammatory Mediators in Vitro

  2011cited by this paper
• Innate immune mechanisms for recognition and uptake of Candida species.

  2010cited by this paper
• Epidemiology and outcomes of candidemia in 2019 patients: data from the prospective antifungal therapy alliance registry.

  2009cited by this paper
• The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology.

  2007cited by this paper
• Chemistry and evolution of the Piperaceae

  2007cited by this paper
• Toll-like receptor 4 is a sensor for autophagy associated with innate immunity.

  2007cited by this paper
• Fungal Invasion of Normally Non-Phagocytic Host Cells

  2006cited by this paper
• INFECTION AND IMMUNITY

  2006cited by this paper
• Epidemiology of Candidemia in Brazil: a Nationwide Sentinel Surveillance of Candidemia in Eleven Medical Centers

  2006cited by this paper
• Evaluation of antifungal activity of Piper solmsianum C. DC. var. solmsianum (Piperaceae).

  2005cited by this paper
• Antifungal activity of the extracts and neolignans from Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck

  2005cited by this paper
• Crotalus durissus terrificus Venom Interferes With Morphological, Functional, and Biochemical Changes in Murine Macrophage

  2005cited by this paper
• Benzoic acid derivatives from Piper species and their fungitoxic activity against Cladosporium cladosporioides and C. sphaerospermum.

  2004cited by this paper
• Ternatin, an anti-inflammatory flavonoid, inhibits thioglycolate-elicited rat peritoneal neutrophil accumulation and LPS-activated nitric oxide production in murine macrophages.

  2003cited by this paper
• Activation of Kupffer cells and caspase-3 involved in rat hepatocyte apoptosis induced by endotoxin

  2002cited by this paper
• Candida albicans Hyphal Formation and the Expression of the Efg1-Regulated Proteinases Sap4 to Sap6 Are Required for the Invasion of Parenchymal Organs

  2002cited by this paper
• A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite.

  2001cited by this paper
• Antifungal principles from Piper fulvescens.

  2001cited by this paper
• Antifungal Benzoic Acid Derivatives from Piper Dilatatum.

  1999cited by this paper
• Activation of Kupffer cells and caspase-3 involved in rat hepatocyte apoptosis induced by endotoxin.

  1999cited by this paper
• Phytochemistry of the genus Piper

  1997cited by this paper
• Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi

  1995influential reference
• Nitric oxide synthases in mammals.

  1994cited by this paper
• Nitric oxide. A macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells

  1989cited by this paper
• Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.

  1983cited by this paper

• Organ-Specific Distribution of Antimycobacterial Neolignans in Piper rivinoides and UHPLC-HRMS/MS Analysis of Its Extracts

  2025cites this paper