Virus-induced gene silencing of SlPKY1 attenuates defense responses against gray leaf spot in tomato

Abstract Tomato gray leaf spot caused by Stemphylium lycopersici is a recent epidemic disease. In a previous study, SlPKY1 was obviously upregulated after infection with S. lycopersici in resistant (cv. Motelle) tomato plants. Our study aimed to identify the function of SlPKY1 in response to tomato gray leaf spot. In addition, the expression of SlPKY1 was significantly upregulated in tomato plants sprayed with salicylic acid (SA) and jasmonic acid (JA). Virus-induced gene silencing (VIGS) was used to confirm the function of SlPKY1 in resistant plants. The results showed that SlPKY1-silenced plants were susceptible at 3 days post inoculation (dpi) with increased accumulation of reactive oxygen species and showed changed activities of disease-resistance enzymes. Lactophenol trypan blue staining was used to identify the hypersensitive response (HR) mediated by the resistance protein Sm at key time points after inoculation with S. lycopersici. qRT-PCR was used to explore the possible relationships between SlPKY1 and related defense genes. We found that the silencing of SlPKY1 resulted in attenuated expression of related defense genes after infection with S. lycopersici in tomato plants. Overall, our results indicated that SlPKY1 may be a positive regulator of response to tomato gray leaf spot.

• Publication year

  2020

• Venue

  Scientia Horticulturae

• Publication date

  2020-04-05

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.1016/j.scienta.2019.109149
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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