Establishment of green analytical method for ultrasound-assisted extraction of azadirachtin, mevalonic acid and squalene from cell suspension culture of Azadirachta indica using response surface methodology

Abstract Azadirachta indica is used as a medicinal plant and its biological activity have been studied, but the extraction of its secondary metabolites was not clearly optimized. In this study, response surface methodology was applied for optimization of extraction of azadirachtin, mevalonic acid and squalene from cell suspension culture of A. indica. The Box-Behnken Design was used for the optimization of factors. The azadirachtin, mevalonic acid, and squalene were recognized by HPLC-DAD. Results showed that the optimal condition (solvent, temperature and ultrasonication time) obtained by response surface methodology for target compounds from cell suspension culture of A. indica were as following. Azadirachtin: water, temperature 35 °C and ultrasonication time 20 min, obtained 86.445 mg/g DW; mevalonic acid: 50 % ethanol, temperature 45 °C and ultrasonication time 30 min, provided 33.671 mg/g DW; and squalene: 50 % ethanol, temperature 55 °C and ultrasonication time 10 min, achieved 8.278 mg/g DW.

• Publication year

  2020

• Venue

  Industrial Crops and Products

• Publication date

  2020-02-01

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/j.indcrop.2019.111946
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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