Development of regression model for bacteriocin production from local isolate of Lactobacillus acidophilus MS1 using Box-Behnken design

Abstract Bacteriocins are antimicrobial peptides synthesized by lactic acid bacteria. These are well known for their anti-bacterial activity in fermented foods and extend the shelf-life and safety of food items. The optimum bacteriocin production was predicted from Lactobacillus acidophilus MS1 using Box-Behnken experimental design. The maximum bacteriocin activity titre of 2600AU/mL was observed with 30 °C, pH 6 and 18 h incubation time. The results derived from response surface methodology (RSM) regression equation indicated that the initial pH of the model system influencing stronger than other physical variables at 95% confidence level. Moreover, quadratic and interaction effects of these variables were also found significant. A monomeric peptide of 6.5 kDa was purified to 59.1-fold from crude sample using ion exchange and gel filtration chromatography. Purified peptide showed strong inhibitory activity against biofilm produced by Bacillus cereus, a food born pathogen. Box-Behnken design can be used for large scale production of bacteriocin from novel strain of Lactobacillus acidophilus MS1 that might be used as a preservative and non-allergic antibiotic in food as well as in pharmaceuticals.

• Publication year

  2020

• Venue

  Biocatalysis and agricultural biotechnology

• Publication date

  2020-03-01

• Fields of study

  Agricultural and Food Sciences, Chemistry

• Identifiers
  DOI 10.1016/j.bcab.2020.101542
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Green synthesis, characterization of silver sulfide nanoparticles and antibacterial activity evaluation

  2020cited by this paper
• Novel styryl and aza-styryl cyanine dyes: Synthesis and spectral sensitization evaluation

  2019cited by this paper
• Study on the stability of antioxidant and anti α-glucosidase activities using soaking treatment in Okra (Abelmoschus esculentus L.) mucilage extraction

  2019cited by this paper
• Predictive performance modeling of Habesha brewery wastewater treatment plant using artificial neural networks

  2019cited by this paper
• Bioassays based on higher plants as excellent dosimeters for ecotoxicity monitoring: A review

  2019cited by this paper
• Improvement of Lactobacillus plantarum for the enhanced production of bacteriocin like inhibitory substance using combinatorial approach

  2019cited by this paper
• Current developments of bacteriocins, screening methods and their application in aquaculture and aquatic products

  2019cited by this paper
• Method validation and uncertainty for the determination of rare earth elements, yttrium, thorium and phosphorus in monazite samples by ICP-OES

  2019cited by this paper
• Prevalence of aflatoxin contamination in pulses and spices in different regions of Punjab

  2019cited by this paper
• Identification of Streptomyces spp. isolated from air samples and its cytotoxicity of anti-MRSA bioactive compounds

  2019cited by this paper
• Investigating the Antibacterial Activity of POMA Nanocomposites

  2019cited by this paper
• Probiotic characterization of indigenous Bacillus velezensis strain DU14 isolated from Apong, a traditionally fermented rice beer of Assam

  2019cited by this paper
• Activity of antibacterial compounds from Bacillus subtilis against cellular oncoproteins by in silico approach

  2019cited by this paper
• Incorporation of microencapsulated Lactobacillus rhamnosus into infant-foods inhibit proliferation of toxicogenic Bacillus cereus strains

  2019cited by this paper
• Activated carbon from molasses efficiency for Cr ( VI ) , Pb ( II ) and Cu ( II ) adsorption : A mechanistic study

  2018cited by this paper
• The role of probiotic Lactobacillus acidophilus ATCC 4356 bacteriocin on effect of HBsu on planktonic cells and biofilm formation of Bacillus subtilis.

  2018cited by this paper
• methodological trends in preparation of activated carbon from local sources and their impacts on production a review

  2018cited by this paper
• Zn-doped SiO2 nanoparticles preparation and characterization under the effect of various solvents: Antibacterial, antifungal and photocatlytic performance evaluation.

  2018cited by this paper
• Predictive Performance Modeling of Habesha Brewery’s Wastewater Treatment Plant Using Artificial Neural Networks

  2018cited by this paper
• UV spectroscopic method for determination of phenytoin in bulk and injection forms

  2018cited by this paper
• Voltammetric study of secnidazole and its determination in pharmaceutical tablet using 1 , 4-benzoquinone modified carbon paste electrode

  2018cited by this paper
• Green synthesis of iron nanoparticles using flower extract of Piliostigma thonningii and their antibacterial activity evaluation

  2018cited by this paper
• Adsorption of 2 , 4 , 6-trichlorophenol on bentonite modified with benzyldimethyltetradecylammonium chloride

  2018cited by this paper
• Elaboration of novel adsorbent from Moroccan oil shale using Plackett–Burman design

  2018cited by this paper
• Aflatoxins biosynthesis , toxicity and intervention strategies : A review

  2018cited by this paper
• Toxicological symptoms and leachates quality in Elelenwo , Rivers State , Nigeria

  2018cited by this paper
• Purification and characterization of mejucin, a new bacteriocin produced by Bacillus subtilis SN7

  2018cited by this paper
• Variations in the Physicochemical Profileof Khushab Coal under Various EnvironmentalConditions

  2018cited by this paper
• Isolation, identification and extraction of antimicrobial compounds produced by Streptomyces sps from terrestrial soil

  2018cited by this paper
• Halocin HA1: An archaeocin produced by the haloarchaeon Haloferax larsenii HA1

  2017cited by this paper
• Coal desulfurization using gamma and ultraviolet radiation

  2017cited by this paper
• Validated UV-Visible spectrometry using water as a solvent for determination of chloroquine in tablet samples

  2017cited by this paper
• Microwave-assisted desulfurization of coal by photo-catalytic oxidation treatment

  2017cited by this paper
• Strain improvement of newly isolated Lactobacillus acidophilus MS1 for enhanced bacteriocin production

  2017cited by this paper
• Survey of Residual Pesticides in Various FreshFruit Crops: A Case Study

  2017cited by this paper
• Coal desulphurization and conditions optimization through response surface methodology, Khushab mines, Pakistan

  2017cited by this paper
• Synthesis, photosensitization and antimicrobial activity evaluation of some novel Merocyanine dyes

  2016cited by this paper
• Purification and Partial Characterization of a Novel Bacteriocin Synthesized by Lactobacillus paracasei HD1-7 Isolated from Chinese Sauerkraut Juice

  2016cited by this paper
• Investigation on growth, structure and characterization of succinate salt of 8-hydroxyquinoline: an organic NLO crystal.

  2015cited by this paper
• Purification and Characterization of Bacteriocin Produced by Weissella confusa A3 of Dairy Origin

  2015cited by this paper
• Effect of Lactobacillus acidophilus NS1 on plasma cholesterol levels in diet-induced obese mice.

  2015cited by this paper
• Photo-degradation of monoazo dye blue 13 using advanced oxidation process

  2015cited by this paper
• Antimicrobial Activity of Lactic Acid Bacteria in Dairy Products and Gut: Effect on Pathogens

  2015cited by this paper
• Optimization of antimicrobial substances produced from Lactobacillus paracasei subsp. paracasei NTU 101 (DSM 28047) and Lactobacillus plantarum NTU 102 by response surface methodology

  2015cited by this paper
• Box–Behnken design optimization for the removal of Direct Violet 51 dye from aqueous solution using lignocellulosic waste

  2015cited by this paper
• Cholesterol-lowering effects of a putative probiotic strain Lactobacillus plantarum EM isolated from kimchi

  2015cited by this paper
• Study on the stability of antioxidant and anti u-glucosidase activities using soaking treatments in Okra (abelmoschus esculentus l.) Mucilage extraction

  2014cited by this paper
• Simultaneous Production of Biosurfactants and Bacteriocins by Probiotic Lactobacillus casei MRTL3

  2014cited by this paper
• Antimicrobial protein produced by pseudomonas aeruginosa JU-Ch 1, with a broad spectrum of antimicrobial activity

  2014cited by this paper
• Strategies to improve the bacteriocin protection provided by lactic acid bacteria.

  2013cited by this paper
• Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS.

  2013cited by this paper
• Study Bacteriocin Production and Optimization Using New Isolates of Lactobacillus spp. Isolated from Some Dairy Products under Different Culture Conditions

  2013cited by this paper
• Antilisterial Activity of Nisin-Like Bacteriocin-Producing Lactococcus lactis subsp. lactis Isolated from Traditional Sardinian Dairy Products

  2012cited by this paper
• Statistical optimization of physical parameters for enhanced bacteriocin production by L. casei

  2012cited by this paper
• Partial Characterisation Of Bacteriocins Produced By Lactic Acid Bacteria Isolated From Thai Fermented Meat And Fish Products

  2012cited by this paper
• Simple and rapid purification of pediocin PA-1 from Pediococcus pentosaceous NCDC 273 suitable for industrial application.

  2012cited by this paper
• Application of response surface methodology in the optimisation of a growth medium for enhanced natural preservative bacteriocin production by a probiotic bacterium

  2012cited by this paper
• Streptomyces scopuliridis sp. nov., a bacteriocin-producing soil streptomycete.

  2011cited by this paper
• Optimization of bacteriocin production by Pediococcus acidilactici MPKI using response surface methodology

  2011cited by this paper
• Optimization of bacteriocin production by Lactobacillus acidophilus AA11, a strain isolated from Egyptian cheese

  2011cited by this paper
• Fate and efficacy of lacticin 3147-producing Lactococcus lactis in the mammalian gastrointestinal tract.

  2011cited by this paper
• The use of response surface methodology in optimization of lactic acid production: focus on medium supplementation, temperature and pH control.

  2010cited by this paper
• Characterization of bacteriocins produced by two strains of Lactobacillus plantarum isolated from Beloura and Chouriço, traditional pork products from Portugal.

  2010cited by this paper
• UV-induced mutagenesis and bioleaching of Acidiphilium cryptum and Acidithiobacillus ferrooxidans

  2010cited by this paper
• Optimization of nisin production by Lactococcus lactis UQ2 using supplemented whey as alternative culture medium.

  2010cited by this paper
• Isolation and characterization of plantaricin ASM1: a new bacteriocin produced by Lactobacillus plantarum A-1.

  2010cited by this paper
• Purification and Characterization of a Bacteriocin Produced by Lactobacillus lactis Isolated from Marine Environment

  2010cited by this paper
• Response surface methodology (RSM) as a tool for optimization in analytical chemistry.

  2008cited by this paper
• Box-Behnken design: an alternative for the optimization of analytical methods.

  2007cited by this paper
• Purification and characterisation of acidocin D20079, a bacteriocin produced by Lactobacillus acidophilus DSM 20079.

  2005cited by this paper
• Bacteriocin production by Bacillus licheniformis strain P40 in cheese whey using response surface methodology

  2004cited by this paper
• Effects of aeration and pH gradient on nisin production.: A mathematical model

  2001cited by this paper
• Classification and mode of action of membrane-active bacteriocins produced by gram-positive bacteria

  2001cited by this paper
• A modified microtiter-plate test for quantification of staphylococcal biofilm formation.

  2000cited by this paper
• Purification and characterization of a bacteriocin produced by Lactobacillus acidophilus IBB 801

  1999cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Some New Three Level Designs for the Study of Quantitative Variables

  1960cited by this paper
• Adsorption of Cu ( II ) , Ni ( II ) and Zn ( II ) ions by nano kaolinite : Thermodynamics and kinetics studies

  year unknowncited by this paper

• Bacteriocin Production by Lactiplantibacillus plantarum LD1 in Solid-State Fermentation Using Lignocellulosic Substrates

  2025cites this paper
• Optimization and purification of a novel bacteriocin-like substance from Tarhana-originated Enterococcus faecium.

  2025cites this paper
• Removal of saponins from sugar factory wastewater using aluminum sulfate, ferric chloride, and natural zeolite coagulants

  2025cites this paper
• Inhibitory efficacy, production dynamics, and characterization of postbiotics of lactic acid bacteria

  2025cites this paper
• Characterization of natural therapeutic compounds producing novel bacterial strains isolated from Hyderabad, India

  2025cites this paper
• Statistical Optimization of Culture Conditions for Enhanced Biomass Yield of Lactobacillus acidophilus CM1 Using Plackett–Burman and Response Surface Methodology

  2025cites this paper
• A Review on Bacteriocin Extraction Techniques from Lactic Acid Bacteria

  2024cites this paper
• Investigation of vitality, antibacterial properties, and antagonistic effects of probiotic bacteria in probiotic dairy products

  2024cites this paper
• A Response Surface Methodological Approach for Large-Scale Production of Antibacterials from Lactiplantibacillus plantarum with Potential Utility against Foodborne and Orthopedic Infections

  2024cites this paper
• Rerouting the Metabolic Pathway in Lactococcus lactis ATCC 11454 to Improve Nisin Productivity by Disrupting Lactate Dehydrogenase (ldh)

  2024cites this paper
• Development of New Media for Production of Bacteriocin from Weissella cibaria man1

  2024cites this paper
• Development a modified MRS medium for enhanced growth of psychrotrophic lactic acid bacteria isolated from kimchi

  2024cites this paper
• Agonistic antibacterial potential of Loigolactobacillus coryniformis BCH-4 metabolites against selected human pathogenic bacteria: An in vitro and in silico approach

  2023cites this paper
• Use of the Box–Behnken Experimental Design for the Optimization of Orange II (Acid Orange 7) Adsorption on Aloe vera

  2023cites this paper
• Hemolytic activity and protective potential of Lamiaceae plants seed extracts and their bioactive components profiling having potential for functional foods and nutraceutical formulations

  2022cites this paper
• Characterization of antimicrobial peptides produced by Lactobacillus acidophilus LA-5 and Bifidobacterium lactis BB-12 and their inhibitory effect against foodborne pathogens

  2022cites this paper
• Advances and New Perspectives in Prebiotic, Probiotic and Symbiotic Products for Food Nutrition and Feed

  2022cites this paper
• ZIF-67 nanocrystals for determining silver: optimizing conditions by Box–Behnken design

  2022cites this paper
• Vacuum impregnation on apples with grape juice concentrate: Effects of pressure, processing time, and juice concentration

  2022cites this paper
• Physicochemical Traits, Variation in Oil Contents and Comparative Analysis of Selected Varieties of Olea Europaea Cultivated under Specific Agro-Ecological Conditions

  2022cites this paper
• Characterization of Pseudoalteromonas sp. from Aquaculture Environment and Optimization of Fermentation Culture Parameters by RSM-Based Modeling

  2022cites this paper
• The impact of incorporating Lactobacillus acidophilus bacteriocin with inulin and FOS on yogurt quality

  2022cites this paper
• Effects of Individual and Combined Fermentation Factors on Antimicrobial Activity of Nisin by Lactococcus lactis ATCC 11454

  2022cites this paper
• High-Frequency Pulsatile Parameterization Study for the Titania Ceramic Membrane Fouling Mitigation in Oily Wastewater Systems Using the Box–Behnken Response Surface Methodology

  2022cites this paper
• Used central composite chemometric method to adsorption gentian violet dye by nano activated charcoal

  2021cites this paper
• In vitro & In vivo Phytochemical Evaluation of Bioactive Components Against Hyperglycemic-induced Oxidative Stress in Streptozocin Rat Model: A histopathological investigation

  2021cites this paper
• Bacteriocin: A New Strategic Antibiofilm Agent in Food Industries

  2021cites this paper
• Used Chemometric Method to Adsorption Fast Green Dye by Nano Charcoal Activated

  2021cites this paper
• Removal Congo Red Dye on Surface of Nano Charcoal Activated by Used Central Composite Chemometric Method

  2021cites this paper
• Property Enhancement of Mustard Stalk Biomass by Torrefaction: Characterization and Optimization of Process Parameters Using Response Surface Methodology

  2021cites this paper
• Definitive screening design (DSD) optimization of methyl orange adsorption onto Moroccan clay

  2021cites this paper
• Versatility and effectiveness of the commercial composts for ecological restoration of heavy metal contaminated soil for sunflower crop

  2021cites this paper
• Low concentrated phosphorus sorption in aqueous medium on aragonite synthesized by carbonation of seashells: Optimization, kinetics, and mechanism study.

  2020cites this paper
• Development of New Media for the Production of Bacteriocin from Weissella

  year unknowncites this paper