Deneysel Alerjik Konjonktivit Modelinde Çörek Otu Bileşenlerinden Timokinon'un Etkisi

Amac: Alerjik konjonktivit tedavisi guc bir hastaliktir; yeni tedavi seceneklerine ihtiyac vardir. Bu calismada anti-inflamatuar ve anti-alerjik ozellikleri olan ve corek otundan (Nigella Sativa) elde edilen timokinonun (TK) sican alerjik konjonktivit (AK) modeli uzerindeki etkisinin histolojik yontemlerle arastirilmasi amaclanmistir. Yontem: Calismaya 48 adet 4-6 aylik yetiskin Wistar Albino disi sican dahil edildi. Sicanlar; kontrol, AK, AK+Deksametazon, 10 mg/kg TK, AK+3 mg/kg TK ve AK+10 mg/kg TK seklinde rastgele 6 gruba ayrildi. AK gelistirmek icin, 30 mg/ml adjuvan Al(OH) 3  ve 0,3 mg Ovalbumin, serum fizyolojik icinde her sicana 1 mL intraperitoneal (i.p.) olarak gun asiri 7 kez uygulandi. Ardi sira 15-21. gunlerde dogrudan konjonktiva uzerine serum fizyolojik icinde %2'lik Ovalbumin 7 kez damlatildi. Ait oldugu gruba gore TK ilgili dozlarda i.p. olarak uygulandi. Konjonktiva dokusu mast hucre sayilari (MHS) ile histopatolojik ve immunohistokimyasal MCT, TGFβ ve TNFα boyama sonuclari istatistiksel olarak degerlendirildi. Bulgular: Timokinonun tek basina sistemik uygulanmasi konjonktiva histolojisinde degisiklige neden olmadi. Ovalbuminle duyarli hale getirilen AK grubu kontrol grubuyla karsilastirildiginda, konjonktiva epitelindeki MHS istatistiksel olarak anlamli artis gosterdi ( p <0,001). Deksametazon tedavi grubunda ( p <0,001) oldugu gibi; TK tedavi gruplarinda da MHS, AK grubuna gore daha dusuk bulundu ancak bu fark istatistiksel olarak anlamli degildi. TGF-β ile negatif boyanma, TNF-α ile zayif fokal boyanma tedavi gruplarinin ortak bulgusu olarak saptandi. Sonuc: Bu calismadaki doz ve surelerle uygulanan ovalbumin, deneysel calismalar icin uygun bir AK modeli olusturmaktadir. Timokinonun uygulanan dozlarda yan etki olusturmadigi ve AK gelisen durumlarda da kismen anti-inflamatuar yonu ile tedaviye destek olabilecegi, ancak istenen anti-alerjik etkiyi gostermedigi dusunulmektedir.

• Publication year

  2020

• Venue

  Unknown venue

• Publication date

  2020-01-12

• Fields of study

  Not labeled

• Identifiers
  DOI 10.30934/kusbed.596688
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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