Microbial source tracking

Orin C. Shanks,A. Korajkic

Published 2020 in Microbial Forensics

ABSTRACT

Abstract Human waste can harbor disease-causing pathogens that can compromise public health and lead to economic burdens for any community that relies on clean and safe water resources. Regulators and public health officials rely on general fecal indicator microorganisms (e.g., Escherichia coli and enterococci) to monitor fecal pollution levels. Methodologies to detect and quantify these microorganisms are designed to measure the total level of fecal pollution in water. However, these assays are unable to discriminate between human and other potential animal sources thus limiting rapid identification and remediation of human pollution sources. Recent advances in molecular biology have led to the development of human fecal source identification tools with the potential to dramatically improve water quality and safety management. This chapter describes the development, validation, and implementation of the human-associated HF183 quantitative real-time PCR technology for water quality and public health protection applications.

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REFERENCES

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