Combination treatment of MCF-7 spheroids by Pseudomonas aeruginosa HI1 levan and cisplatin

Abstract Treatment of cancer with chemotherapy drugs is a challenge because of drug resistance and severe side effects. This study aimed to evaluate the cytotoxicity of levan as a natural biopolymer alone and in combination with cisplatin. Consequently, honey isolate NRCA, Pseudomonas aeruginosa, recorded promising levansucrase activity and levan yield. Crude levan (CL) was dialyzed to dialyze levan (DL). Crude levan showed cytotoxicity effect by 69.5%, 74% on MCF-7 at 500 and 1000 μg/ml, respectively while it has a safe effect on normal cells. Dialyzed levan increased cancer cell viability. Combination treatment of CL and cisplatin significantly reduced the IC50 of cisplatin (9.4 μM) to 5.8 μM and 3.8 μM when combined with Levan 500 μg/ml and 1000 μg/ml, respectively. Crude levan cytotoxicity is a dose-independent with IC50 447 μg/ml. Levan did not cause DNA fragmentation in treated cells. Accordingly, treatment design to treat spheroids with a combination of both CL and cisplatin at different time intervals was suggested. This design was found to increase the cytotoxicity against MCF-7 spheroids and reduce the cytotoxicity of cisplatin on spheroids of normal cell line. It can be concluded that combination treatment of cisplatin and levan might decrease the IC50 of cisplatin and overcome side effects problems.

• Publication year

  2020

• Venue

  Biocatalysis and agricultural biotechnology

• Publication date

  2020-03-01

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/j.bcab.2020.101526
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Cisplatin-functionalized three-dimensional magnetic SBA-16 for treating breast cancer cells (MCF-7)

  2019cited by this paper
• SPIONs/3D SiSBA-16 based Multifunctional Nanoformulation for target specific cisplatin release in colon and cervical cancer cell lines

  2019cited by this paper
• Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan

  2019cited by this paper
• Deposition of calcium in an in vitro model of human breast tumour calcification reveals functional role for ALP activity, altered expression of osteogenic genes and dysregulation of the TRPM7 ion channel

  2019cited by this paper
• Biological Activity of Levan Produced from Rhizospheric Soil Bacterium Brachybacterium phenoliresistens KX139300

  2018cited by this paper
• Optimization of Enterococcus faecalis Esawy KR758759 dextransucrase and evaluation of some dextran bioactivities

  2018cited by this paper
• Microbial levan from Brachybacterium phenoliresistens: Characterization and enhancement of production

  2017cited by this paper
• Optimization of Levan Production by Cold-Active Bacillus licheniformis ANT 179 and Fructooligosaccharide Synthesis by Its Levansucrase

  2017cited by this paper
• Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress.

  2017cited by this paper
• A systematic multicellular spheroids screening approach lead to the identification of antineoplastic activity in three different plant extracts from the Egyptian flora

  2017cited by this paper
• Microbial Biosynthesis of Health-Promoting Food Ingredients

  2017cited by this paper
• Potassium increases the antitumor effects of ascorbic acid in breast cancer cell lines in vitro.

  2016cited by this paper
• Self-assembled levan nanoparticles for targeted breast cancer imaging.

  2015cited by this paper
• Isolation and Characterization of Levan from Moderate Halophilic Bacteria Bacillus licheniformis BK AG21

  2015cited by this paper
• Present and future medical applications of microbial exopolysaccharides

  2015cited by this paper
• Developing multi-target therapeutics to fine-tune the evolutionary dynamics of the cancer ecosystem

  2015cited by this paper
• Genome Sequence of the Nonpathogenic Pseudomonas aeruginosa Strain ATCC 15442

  2014cited by this paper
• Cisplatin in cancer therapy: molecular mechanisms of action.

  2014cited by this paper
• Investigation of anti-cancer activity of linear and aldehyde-activated levan from Halomonas smyrnensis AAD6T

  2014cited by this paper
• Scaling up, characterization of levan and its inhibitory role in carcinogenesis initiation stage'.

  2013cited by this paper
• Structural determination and NMR characterization of a bacterial exopolysaccharide.

  2013cited by this paper
• Microbial levan from Pseudomonas fluorescens: Characterization and medium optimization for enhanced production

  2012cited by this paper
• Antiviral Levans from Bacillus spp. Isolated from Honey

  2012cited by this paper
• Optimization of enzymatic production of fructooligosaccharides from longan syrup

  2012cited by this paper
• Pseudomonas biofilm matrix composition and niche biology.

  2012cited by this paper
• Characterization of Extracellular Dextranase from a Novel Halophilic Bacillus subtilis NRC-B233b a Mutagenic Honey Isolate under Solid State Fermentation

  2012cited by this paper
• Antitumor and antioxidant activities of levan and its derivative from the isolate Bacillus subtilis NRC1aza.

  2012cited by this paper
• Identification of Agents that Induce Apoptosis of Multicellular Tumour Spheroids: Enrichment for Mitotic Inhibitors with Hydrophobic Properties

  2011cited by this paper
• Production of levansucrase from novel honey Bacillus subtilis isolates capable of producing antiviral levans

  2011cited by this paper
• Real-Time Monitoring of Cisplatin-Induced Cell Death

  2011cited by this paper
• GLUT 5 Is Not Over-Expressed in Breast Cancer Cells and Patient Breast Cancer Tissues

  2011cited by this paper
• Cancer Therapeutics- Opportunities, Challenges and Advances in Drug Delivery

  2011cited by this paper
• Non-toxic Pseudomonas aeruginosa exotoxin A expressing the FMDV VP1 G-H loop for mucosal vaccination of swine against foot and mouth disease virus.

  2007cited by this paper
• A Reliable Tool to Determine Cell Viability in Complex 3-D Culture: The Acid Phosphatase Assay

  2007cited by this paper
• Fluorescent fructose derivatives for imaging breast cancer cells.

  2007cited by this paper
• Properties of levan and potential medical uses

  2006cited by this paper
• Strategies for optimizing combinations of molecularly targeted anticancer agents

  2006cited by this paper
• Analysis of DNA fragmentation using agarose gel electrophoresis.

  2006cited by this paper
• selective production and characterization of levan by Bacillus subtilis (Natto) Takahashi.

  2005cited by this paper
• Effect of levan's branching structure on antitumor activity.

  2004cited by this paper
• Classification of anticancer drugs--a new system based on therapeutic targets.

  2003cited by this paper
• Cloning, sequencing, and characterization of the intracellular invertase gene from Zymomonas mobilis.

  1991cited by this paper
• 16S ribosomal DNA amplification for phylogenetic study

  1991cited by this paper
• Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.

  1983cited by this paper
• Effects of route and schedule of administration of high-molecular levan on the growth of AKR lymphoma.

  1976cited by this paper

• Dose-dependent antiulcerogenic effect of bacterial levans on indomethacin-induced gastric lesions through modulation of NF-κB/TNF-α expression and apoptotic pathways.

  2025cites this paper
• Potential antibacterial, antioxidant and anticancer activities of Nigerian mountain honey in relation to its bacterial flora

  2025cites this paper
• Enzymatic synthesis of biologically active fructose-based saccharides by Aspergillus niger MK788296 levansucrase

  2024cites this paper
• Unraveling the mechanism of the anticancer potential of emodin using 2D and spheroid models of A549 cells.

  2024cites this paper
• Protein aggregation monitoring in cells under oxidative stress: a novel fluorescent probe based on a 7-azaindole-BODIPY derivative.

  2024cites this paper
• Prebiotic-mediated gastroprotective potentials of three bacterial levans through NF-κB-modulation and upregulation of systemic IL-17A.

  2023cites this paper
• Hibiscus-cisplatin combination treatment decreases liver toxicity in rats while increasing toxicity in lung cancer cells via oxidative stress- apoptosis pathway.

  2023cites this paper
• Biological activities and structural characterization of sulfated polysaccharide extracted from a newly Mediterranean Sea record Grateloupia gibbesii Harvey

  2022cites this paper
• Production and bioactivities of nanoparticulated and ultrasonic-degraded levan generated by Erwinia tasmaniensis levansucrase in human osteosarcoma cells.

  2022cites this paper
• Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.

  2021cites this paper
• Synergistic enzyme cocktail between levansucrase and inulosucrase for superb levan-type fructooligosaccharide synthesis.

  2021cites this paper
• Evaluation of different bacterial honey isolates as probiotics and their efficient roles in cholesterol reduction

  2021cites this paper
• A Fluorescent Probe for Protein Misfolding and Aggregation Due to Oxidative Stress Based on a 7-Azaindole-BODIPY Derivative

  year unknowncites this paper