Emergence of Multidrug-Resistant Staphylococci on Hand-Touching Surfaces in a University Town in Southwestern China: A Prevalence Study of Virulence and Resistance

Background: Staphylococcus aureus and coagulase-negative staphylococci are highly antibiotic-resistant pathogens and exhibit multiple virulence factors that threaten public health. However, few reports have evaluated the resistance and virulence of staphylococci in community settings. Objectives: This study surveyed the epidemiology, antimicrobial resistance, and virulence genes of staphylococci in the densely-populated university town of Kunming in Southwestern China. Methods: Various strains were isolated from hand-touching surfaces in Chenggong University Town, China, and identified via 16S rRNAsequencing. Staphylococciwereisolated,anddrugresistancewasdeterminedviaantibioticsensitivitytesting. The mecA gene was verified and typed. Twelve virulence-associated genes and several disinfectant-resistance genes were determined. Results: One hundred sixty-three strains were collected from hand-touching surfaces, and more than a quarter of the isolates (46/163, 28.22%) were identified as staphylococci. Among the staphylococcal strains, 19 (41.30%) showed multidrug resistance, with resistance rates of more than 40% to benzyl-penicillin, erythromycin, oxacillin, and tetracycline. Twenty isolates (43.48%) carried mecA , and most staphylococcal cassette chromosome mecs ( SCCmecs ) were nontypeable. Twenty-eight strains (60.87%) carried at least one virulence gene; hemolysin genes were the most prevalent, and 22 strains (47.83%) carried qac genes. Staphylococcus aureus strain ST59 carried multiple resistance and virulence genes. Conclusions: Resistant staphylococci can easily adhere to hand-touching surfaces. More attention should be given to the appear-ance of resistance and virulence gene-harboring staphylococci in community settings. The findings herein suggest that effective disinfectant strategies and feasible surveillance measures are urgently needed.

• Publication year

  2019

• Venue

  Jundishapur Journal of Microbiology

• Publication date

  2019-12-17

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.5812/jjm.95859
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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