The occurrence of a glucose-enzyme complex in the reaction catalyzed by sucrose phosphorylase.

J. Voet,R. Abeles

Published 1966 in Journal of Biological Chemistry

ABSTRACT

Abstract Evidence has been obtained for the formation of a glucose-enzyme complex in reactions catalyzed by sucrose phosphorylase isolated from Pseudomonas saccharophila. When sucrose phosphorylase is denatured after exposure to uniformly labeled sucrose-14C, the denatured protein contains firmly bound d-glucose or a compound derived from the d-glucose moiety of sucrose. The d-fructose moiety of sucrose is not bound to the protein.

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