Reversible Assembly of the ATP-binding Cassette Transporter Mdl1 with the F1F0-ATP Synthase in Mitochondria*

The half-ABC transporter Mdl1 is localized in the inner membrane of mitochondria and mediates the export of peptides generated upon proteolysis of mitochondrial proteins. The physiological role of the peptides released from mitochondria is currently not understood. Here, we have analyzed the oligomeric state of Mdl1 in the inner membrane and demonstrate nucleotide-dependent binding to the F1F0-ATP synthase. Mdl1 forms homo-oligomeric, presumably dimeric complexes in the presence of ATP, but was found in association with the F1F0-ATP synthase at low ATP levels. Mdl1 binds membrane-embedded parts of the ATP synthase complex after the assembly of the F1 and F0 moieties. Although independent of Mdl1 activity, complex formation is impaired upon inhibition of the F1F0-ATP synthase with oligomycin or N,N′-dicyclohexylcarbodiimide. These results are consistent with an activation of Mdl1 upon dissociation from the ATP synthase and suggest a link of peptide export from mitochondria to the activity of the F1F0-ATP synthase and the cellular energy metabolism.

• Publication year

  2004

• Venue

  Journal of Biological Chemistry

• Publication date

  2004-09-10

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/JBC.M405871200PMID 15247210
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Atp10p Assists Assembly of Atp6p into the F0 Unit of the Yeast Mitochondrial ATPase*

  2004cited by this paper
• Chapter 25 - ABC Transporters in Mitochondria

  2003cited by this paper
• Retrograde signaling is regulated by the dynamic interaction between Rtg2p and Mks1p.

  2003cited by this paper
• ABC proteins : from bacteria to man

  2003cited by this paper
• CHAPTER 6 – PROBING OF CONFORMATIONAL CHANGES, CATALYTIC CYCLE AND ABC TRANSPORTER FUNCTION

  2003cited by this paper
• The ATP Hydrolysis Cycle of the Nucleotide-binding Domain of the Mitochondrial ATP-binding Cassette Transporter Mdl1p*

  2003cited by this paper
• MDL1 is a high copy suppressor of ATM1: evidence for a role in resistance to oxidative stress.

  2003cited by this paper
• Structure and mechanism of ABC transporters.

  2002cited by this paper
• Membrane protein degradation by AAA proteases in mitochondria.

  2002cited by this paper
• Genome‐wide studies on the nuclear PDR3‐controlled response to mitochondrial dysfunction in yeast

  2002cited by this paper
• A mitochondrial specific stress response in mammalian cells

  2002cited by this paper
• CREB activation induced by mitochondrial dysfunction is a new signaling pathway that impairs cell proliferation

  2002cited by this paper
• Conservation of amino acid transporters in fungi, plants and animals.

  2002cited by this paper
• The ATP synthase is involved in generating mitochondrial cristae morphology

  2002cited by this paper
• Mitochondria‐to‐nucleus stress signaling induces phenotypic changes, tumor progression and cell invasion

  2001cited by this paper
• Blue-native gels to isolate protein complexes from mitochondria.

  2001cited by this paper
• Role of the ABC Transporter Mdl1 in Peptide Export from Mitochondria

  2001cited by this paper
• Protein degradation in mitochondria: implications for oxidative stress, aging and disease: a novel etiological classification of mitochondrial proteolytic disorders.

  2001cited by this paper
• Membrane protein degradation by AAA proteases in mitochondria: extraction of substrates from either membrane surface.

  2000cited by this paper
• ABC‐me: a novel mitochondrial transporter induced by GATA‐1 during erythroid differentiation

  2000cited by this paper
• M‐ABC2, a new human mitochondrial ATP‐binding cassette membrane protein

  2000cited by this paper
• The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins

  1999cited by this paper
• Dislocation of membrane proteins in FtsH‐mediated proteolysis

  1999cited by this paper
• Identification and characterization of a mammalian mitochondrial ATP-binding cassette membrane protein.

  1999cited by this paper
• Yeast mitochondrial F1F0‐ATP synthase exists as a dimer: identification of three dimer‐specific subunits

  1998cited by this paper
• The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m‐AAA protease

  1998cited by this paper
• Mutations in RCA1 and AFG3 inhibit F1‐ATPase assembly in Saccharomyces cerevisiae

  1995cited by this paper
• Molecular chaperones cooperate with PIM1 protease in the degradation of misfolded proteins in mitochondria.

  1994cited by this paper
• XIIXVI. Yeast sequencing reports. Mapping and sequencing of two yeast genes belonging to the ATP‐binding cassette superfamily

  1994cited by this paper
• Characterization of ATP12, a yeast nuclear gene required for the assembly of the mitochondrial F1-ATPase.

  1991cited by this paper
• Analysis and manipulation of yeast mitochondrial genes.

  1991cited by this paper
• ATP10, a yeast nuclear gene required for the assembly of the mitochondrial F1-F0 complex.

  1990cited by this paper
• The mitochondrial genotype can influence nuclear gene expression in yeast.

  1987cited by this paper
• Liver mitochondria contain an ATP-dependent, vanadate-sensitive pathway for the degradation of proteins.

  1982cited by this paper

• Mitochondrial Inner Membrane ABC Transporter Bcmdl1 Is Involved in Conidial Germination, Virulence, and Resistance to Anilinopyrimidine Fungicides in Botrytis cinerea

  2023influential citation
• Site-directed transformants with E407K substitution in Bcmdl1 possesses different fitness from field anilinopyrimidine resistant isolates with E407K mutation in Botrytis cinerea.

  2023cites this paper
• Down the Iron Path: Mitochondrial Iron Homeostasis and Beyond

  2021cites this paper
• Anilinopyrimidine Resistance in Botrytis cinerea Is Linked to Mitochondrial Function

  2017cites this paper
• Compartmentalization of iron between mitochondria and the cytosol and its regulation.

  2015cites this paper
• Structural, functional and molecular docking study to characterize GMI1 from Arabidopsis thaliana

  2013cites this paper
• Mitochondrial AAA proteases--towards a molecular understanding of membrane-bound proteolytic machines.

  2012cites this paper
• Targeting the F1Fo ATP Synthase: modulation of the body's powerhouse and its implications for human disease.

  2011cites this paper
• ATP synthase superassemblies in animals and plants: two or more are better.

  2011cites this paper
• Efficient Phagocytosis Requires Triacylglycerol Hydrolysis by Adipose Triglyceride Lipase

  2010cites this paper
• Author ' s personal copy Mitochondrial ABC proteins in health and disease

  2009cites this paper
• Mitochondrial ABC proteins in health and disease.

  2009cites this paper
• This information is current as Mitochondrial Integrity Macrophage ATP Production and Constitutive ERK MAPK Activity Regulates

  2008cites this paper
• Constitutive ERK MAPK Activity Regulates Macrophage ATP Production and Mitochondrial Integrity1

  2008cites this paper
• Functional analysis of mammalian m-AAA proteases

  2008cites this paper
• Functional characterization of the mammalian iAAA protease subunit, YME1L

  2008cites this paper
• Mitochondrial ATP-binding cassette proteins.

  2007cites this paper
• Oxa1 directly interacts with Atp9 and mediates its assembly into the mitochondrial F1Fo-ATP synthase complex.

  2007cites this paper
• Protein Degradation within Mitochondria: Versatile Activities of AAA Proteases and Other Peptidases

  2007cites this paper
• The significance of Arabidopsis AAA proteases for activity and assembly/stability of mitochondrial OXPHOS complexes

  2007cites this paper
• Structural Evolution of the ABC Transporter Subfamily B

  2007cites this paper
• Intracellular peptide transporters in human – compartmentalization of the “peptidome”

  2007cites this paper
• Prohibitins interact genetically with Atp23, a novel processing peptidase and chaperone for the F1Fo-ATP synthase.

  2006cites this paper
• Evidence for a novel mitochondria-to-nucleus signalling pathway in respiring cells lacking i-AAA protease and the ABC-transporter Mdl1.

  2006cites this paper
• Yeast ABC transporters – A tale of sex, stress, drugs and aging

  2006cites this paper
• Characterization of Peptides Released from Mitochondria

  2005cites this paper
• The m-AAA protease defective in hereditary spastic paraplegia controls ribosome assembly in mitochondria.

  2005cites this paper
• Characterization of a novel family of nucleobase transporters

  2004cites this paper