Antioxidant activity and components of a traditional chinese medicine formula consisting of Crataegus pinnatifida and Salvia miltiorrhiza

BackgroundThe aims of this study were to evaluate the antioxidant activity and to identify the antioxidant components of a traditional Chinese medicine formula consisting of a combination of Shanzha (the fruit of Crataegus pinnatifida Bge. var. major N.E.Br., SZ) and Danshen (the root of Salvia miltiorrhiza Bge., DS). This medicine is extensively used to treat cardiovascular disease.MethodsTwelve samples extracted and fractionated from SZ, DS and the formula (SZ+DS) were analyzed. The concentrations of eight phenolic compounds were determined by high performance liquid chromatography. Oxygen radical absorbance capacity (ORAC) assay and 1,1-diphenyl-2-picrylhydrazyl assay were conducted to explore the antioxidant activities of the samples and of the 15 phenolic compounds detected. Correlation analysis of the antioxidant activity of herb samples and their phenolic components was performed.ResultsThe main phenolic component in all SZ+DS samples was salvianolic acid B, which exhibited strong antioxidant activity (ORAC value: 16.73 ± 2.53, IC50 value: 8.80 ± 0.06 μM) compared with the other phenolic compounds. For all samples, there was a positive relationship between their total phenolic components and their antioxidant activities.ConclusionsPhenolic compounds were the bioactive components of the herb samples, and salvianolic acid B was identified as the main bioactive compound in the SZ+DS formula.

• Publication year

  2013

• Venue

  BMC Complementary and Alternative Medicine

• Publication date

  2013-05-10

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1186/1472-6882-13-99PMID 23663253PMCID 3671134
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• [AAPH scavenging activities of 22 flavonoids and phenolic acids and 9 extracts of Chinese materia medica].

  2010cited by this paper
• Anti-oxidant Activity of Hydrophilic Components of Danshen Root in Vitro

  2009cited by this paper
• [DPPH radical scavenging activities of 31 flavonoids and phenolic acids and 10 extracts of Chinese materia medica].

  2009cited by this paper
• Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B.

  2008cited by this paper
• Antioxidant activity of 45 Chinese herbs and the relationship with their TCM characteristics

  2007cited by this paper
• Study on scavenging activities for superoxide anion radicals (O_2~(?)) and structure-activity relationship of polyphe-nolic compounds from leaves of Crataegus

  2005cited by this paper
• Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements.

  2005cited by this paper
• When east meets west: the relationship between yin‐yang and antioxidation‐oxidation

  2003cited by this paper

• Effect of compound Chinese herbs on growth performance, digestion and serum biochemical indexes of Mandarin fish ( Siniperca chuatsi )

  2026cites this paper
• Inhibitory effect of three components derived from Salvia miltiorrhiza against human cytomegalovirus.

  2026cites this paper
• The Hawthorn (Crataegus spp.) Value Chain: An Integrated Analysis of Resource Availability, Phytochemical Characterization, and Therapeutic Applications

  2026cites this paper
• How to assess antioxidant activity? Advances, limitations, and applications of in vitro, in vivo, and ex vivo approaches

  2025cites this paper
• Comparative efficacy of eight traditional Chinese medicines combined with statins in the treatment of hyperlipidemia: a Bayesian network meta-analysis

  2025cites this paper
• Salvianolic Acid B Alleviates LPS-Induced Spleen Injury by Remodeling Redox Status and Suppressing NLRP3 Inflammasome

  2025cites this paper
• UPLC-MS/MS analysis of Salvia miltiorrhiza Bge. Extract and its ameliorative effect of endothelial cell metabolism in isoproterenol-induced myocardial injury.

  2025cites this paper
• Salvianolic acid extract prevents Tripterygium wilfordii polyglycosides-induced acute liver injury by modulating bile acid metabolism.

  2024cites this paper
• Phytotherapeutic approach to enhance the hepatoprotective activity of some edible plants: Molecular docking and nanoformulation

  2024cites this paper
• Exploring the underlying mechanisms of Danshen-Shanzha Decoction on Coronary Heart Disease: An integrated analysis combining pharmacoinformatics and experimental validation.

  2024cites this paper
• Effects of supplementation with Angelica sinensis Radix and its powder formulation on growth performance, serum antioxidant capacity, immune response and intestinal microflora of broiler chickens

  2024cites this paper
• An Ethnopharmacological Survey on Medicinally Important Plants of Genus Salvia.

  2024cites this paper
• Danshen-Shanzha formula for the treatment of atherosclerosis: ethnopharmacological relevance, preparation methods, chemical constituents, pharmacokinetic properties, and pharmacological effects

  2024cites this paper
• Unveiling the spatial distribution and molecular mechanisms of terpenoid biosynthesis in Salvia miltiorrhiza and S. grandifolia using multi-omics and DESI–MSI

  2023cites this paper
• Systematical targeted multicomponent characterization and comparison of Arnebiae Radix and its three confusing species by offline two-dimensional liquid chromatography/LTQ-Orbitrap mass spectrometry

  2023cites this paper
• Evaluation of anti-HMG-CoA reductase potential and simultaneous determination of phenolic compounds in hydroalcoholic extract of Ficus cunia fruits by RP-HPLC

  2023cites this paper
• Comparative proteomics reveals biochemical changes in Salvia miltiorrhiza Bunge during sweating processing.

  2022cites this paper
• Effect of hawthorn berry pre-treatment and preservation methods on the extractability of color-determining compounds and selected antioxidative substances

  2022cites this paper
• A mechanistic review of chinese medicine polyphenols on bone formation and resorption

  2022cites this paper
• Botany, traditional uses, phytochemistry and pharmacological activity of Crataegus pinnatifida (Chinese hawthorn): a review.

  2022cites this paper
• Neuroprotective Effects of Tanshinone IIA in Experimental Model of Parkinson Disease in Rats

  2022cites this paper
• Saussurea Costus Extract Has Anti-inflammatory, Antioxidant, and Hormonal Effects Against Testicular Toxicity Induced by Oxaliplatin in Male Albino Rats

  2022cites this paper
• Extraction of phenolic compounds from black mustard (Brassica nigra L.) seed by deep eutectic solvents

  2021cites this paper
• Salvia Species as Nutraceuticals: Focus on Antioxidant, Antidiabetic and Anti-Obesity Properties

  2021cites this paper
• Determining the effective compounds of Salvia miltiorrhiza through chromatographic fingerprinting coupled with chemometrics

  2021cites this paper
• Traditional Chinese Medicine is an Alternative Therapeutic Option for Treatment of Pseudomonas aeruginosa Infections

  2021cites this paper
• A novel method for quality consistency evaluation of Yankening Tablet by multi-wavelength fusion profiling combined with overall components dissolution method and antioxidant activity analysis.

  2021cites this paper
• Exploring of Coronilla varia L. extracts as a source of high-value natural agents: Chemical profiles and biological connections

  2021cites this paper
• The Protective Function and Modification of Secondary Metabolite Accumulation in Response to Light Stress in Dracocephalum forrestii Shoots

  2021cites this paper
• Pharmacological Effects of Salvianolic Acid B Against Oxidative Damage

  2020cites this paper
• Marker compound contents and antioxidant capacities of the taproot and lateral root of Danshen (Salvia miltiorrhiza Radix)

  2020cites this paper
• Salvia bowleyana Dunn root is a novel source of salvianolic acid B and displays antitumor effects against gastric cancer cells

  2020cites this paper
• Assessment of the Quality and Evaluation of the Antioxidant Potential of a Novel Sri Lankan Ayurvedic Polyherbal Formulation

  2020cites this paper
• Antioxidant Functionality in Plants and Plant Sourced Biomaterials

  2020cites this paper
• Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves

  2020cites this paper
• Possible anti-oxidative effects of long-term administration of Juzen-taiho-to in dogs

  2019cites this paper
• An anti-influenza virus activity-calibrated chemical standardization approach for quality evaluation of indigo naturalis

  2019cites this paper
• Prevention and Treatment of Chronic Heart Failure through Traditional Chinese Medicine: Role of the Gut Microbiota.

  2019cites this paper
• Analysis of the biodiversity of hawthorn (Crataegus spp.) from the morphological, molecular, and ethnobotanical approaches, and implications for genetic resource conservation in scenery of increasing cultivation: the case of Mexico

  2018cites this paper
• Comparison of the chromatographic fingerprint, multicomponent quantitation and antioxidant activity of Salvia miltiorrhiza Bge. between sweating and nonsweating.

  2018cites this paper
• Ameliorative effects of rutin against cisplatin-induced reproductive toxicity in male rats

  2018cites this paper
• Anti-fatigue and anti-oxidant activities of oyster (Ostrea rivularis) hydrolysate prepared by compound protease.

  2018cites this paper
• Antiseptic Activity of Ethnomedicinal Xuebijing Revealed by the Metabolomics Analysis Using UHPLC-Q-Orbitrap HRMS

  2018cites this paper
• Bioactivities, biosynthesis and biotechnological production of phenolic acids in Salvia miltiorrhiza

  2018cites this paper
• A Review of Chinese Herbal Medicine for the Treatment of Chronic Heart Failure

  2017cites this paper
• Salvia spp.: An Updated on Antioxidant Activity and Pharmacological Uses

  2017cites this paper
• CHEMICAL COMPOSITION AND ANTIOXIDANT CAPACITIES OF FOUR MEDITERRANEAN INDUSTRIAL ESSENTIAL OILS AND THEIR RESULTANT DISTILLED SOLID BY-PRODUCTS†

  2017cites this paper
• Amelioration of cognitive impairments in APPswe/PS1dE9 mice is associated with metabolites alteration induced by total salvianolic acid

  2017cites this paper
• Phytochemical compositions of extract from peel of hawthorn fruit, and its antioxidant capacity, cell growth inhibition, and acetylcholinesterase inhibitory activity

  2017cites this paper
• The making of bispecific antibodies

  2017cites this paper
• The protective effect of procyanidin against LPS-induced acute gut injury by the regulations of oxidative state

  2016cites this paper
• Chemical Analysis of the Herbal Medicine Salviae miltiorrhizae Radix et Rhizoma (Danshen)

  2016cites this paper
• The Pharmacological Potential of Rutin

  2016influential citation
• Salvianolic acid B stimulates osteogenesis in dexamethasone-treated zebrafish larvae

  2016cites this paper
• Salvianolic Acid B Protects Normal Human Dermal Fibroblasts Against Ultraviolet B Irradiation‐Induced Photoaging Through Mitogen‐Activated Protein Kinase and Activator Protein‐1 Pathways

  2015cites this paper
• Methanolic extract of Origanum vulgare ameliorates type 1 diabetes through antioxidant, anti-inflammatory and anti-apoptotic activity.

  2015cites this paper
• Fingerprint analysis, multi-component quantitation, and antioxidant activity for the quality evaluation of Salvia miltiorrhiza var. alba by high-performance liquid chromatography and chemometrics.

  2015cites this paper
• Hawthorn fruit attenuates atherosclerosis by improving the hypolipidemic and antioxidant activities in apolipoprotein e-deficient mice.

  2014cites this paper
• Optimization of the extraction process for the seven bioactive compounds in Yukmijihwang-tang, an herbal formula, using response surface methodology

  2014cites this paper
• Plant derived antioxidants and antifibrotic drugs: past, present and future

  2014cites this paper
• Mechanism of action of Xinmailong, a proprietary Chinese medicine for the treatment of chronic heart failure

  2014cites this paper