Functional Analysis of a Wheat AGPase Plastidial Small Subunit with a Truncated Transit Peptide

ADP-glucose pyrophosphorylase (AGPase), the key enzyme in starch synthesis, consists of two small subunits and two large subunits with cytosolic and plastidial isoforms. In our previous study, a cDNA sequence encoding the plastidial small subunit (TaAGPS1b) of AGPase in grains of bread wheat (Triticum aestivum L.) was isolated and the protein subunit encoded by this gene was characterized as a truncated transit peptide (about 50% shorter than those of other plant AGPS1bs). In the present study, TaAGPS1b was fused with green fluorescent protein (GFP) in rice protoplast cells, and confocal fluorescence microscopy observations revealed that like other AGPS1b containing the normal transit peptide, TaAGPS1b-GFP was localized in chloroplasts. TaAGPS1b was further overexpressed in a Chinese bread wheat cultivar, and the transgenic wheat lines exhibited a significant increase in endosperm AGPase activities, starch contents, and grain weights. These suggested that TaAGPS1b subunit was targeted into plastids by its truncated transit peptide and it could play an important role in starch synthesis in bread wheat grains.

• Publication year

  2017

• Venue

  Molecules

• Publication date

  2017-03-01

• Fields of study

  Biology, Medicine, Materials Science

• Identifiers
  DOI 10.3390/molecules22030386PMID 28257051PMCID 6155376
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• AGPase: its role in crop productivity with emphasis on heat tolerance in cereals

  2015influential reference
• Synthesis of amphoteric starch-based grafting flocculants for flocculation of both positively and negatively charged colloidal contaminants from water

  2014cited by this paper
• The mechanism of ORFH79 suppression with the artificial restorer fertility gene Mt-GRP162.

  2013cited by this paper
• Increasing the starch content and grain weight of common wheat by overexpression of the cytosolic AGPase large subunit gene.

  2013influential reference
• Processing peptidases in mitochondria and chloroplasts.

  2013cited by this paper
• Multigene engineering of starch biosynthesis in maize endosperm increases the total starch content and the proportion of amylose

  2013cited by this paper
• Over-expression of AGPase genes enhances seed weight and starch content in transgenic maize

  2011influential reference
• A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes

  2011cited by this paper
• Genetic gains in grain yield, net photosynthesis and stomatal conductance achieved in Henan Province of China between 1981 and 2008

  2011cited by this paper
• Regulation of Starch Biosynthesis in Response to a Fluctuating Environment1

  2011cited by this paper
• Delivering high-resolution landmarks using inkjet micropatterning for spatial monitoring of leaf expansion

  2011cited by this paper
• Starch biosynthesis in cereal endosperm.

  2010cited by this paper
• A novel Ta.AGP.S.1b transcript in Chinese common wheat (Triticum aestivum L.).

  2010influential reference
• Starch: its metabolism, evolution, and biotechnological modification in plants.

  2010cited by this paper
• Control of starch synthesis in cereals: metabolite analysis of transgenic rice expressing an up-regulated cytoplasmic ADP-glucose pyrophosphorylase in developing seeds.

  2009cited by this paper
• Wheat Genomics: Present Status and Future Prospects

  2008cited by this paper
• Effects of High Temperature on Key Enzymes Involved in Starch and Protein Formation in Grains of Two Wheat Cultivars

  2008cited by this paper
• Locating proteins in the cell using TargetP, SignalP and related tools

  2007cited by this paper
• Two Paralogous Genes Encoding Small Subunits of ADP-glucose Pyrophosphorylase in Maize, Bt2 and L2, Replace the Single Alternatively Spliced Gene Found in Other Cereal Species

  2007cited by this paper
• Expression of a modified ADP-glucose pyrophosphorylase large subunit in wheat seeds stimulates photosynthesis and carbon metabolism

  2007cited by this paper
• Locating proteins in the cell using TargetP, SignalP and related tools

  2007cited by this paper
• Arabidopsis GLUTAMINE-RICH PROTEIN23 Is Essential for Early Embryogenesis and Encodes a Novel Nuclear PPR Motif Protein That Interacts with RNA Polymerase II Subunit III[W]

  2006cited by this paper
• The role of the N‐terminal domain of chloroplast targeting peptides in organellar protein import and miss‐sorting

  2006cited by this paper
• Transgenic wheat progeny resistant to powdery mildew generated by Agrobacterium inoculum to the basal portion of wheat seedling

  2006cited by this paper
• Expression profiling of genes involved in starch synthesis in sink and source organs of rice.

  2005cited by this paper
• The small subunit ADP-glucose pyrophosphorylase (ApS) promoter mediates okadaic acid-sensitive uidA expression in starch-synthesizing tissues and cells in Arabidopsis

  2003cited by this paper
• Subcellular localization of ADPglucose pyrophosphorylase in developing wheat endosperm and analysis of the properties of a plastidial isoform.

  2003cited by this paper
• Directed molecular evolution of ADP-glucose pyrophosphorylase

  2002cited by this paper
• Characterization of the Genes Encoding the Cytosolic and Plastidial Forms of ADP-Glucose Pyrophosphorylase in Wheat Endosperm1

  2002cited by this paper
• Enhanced ADP-glucose pyrophosphorylase activity in wheat endosperm increases seed yield

  2002cited by this paper
• A cytosolic ADP-glucose pyrophosphorylase is a feature of graminaceous endosperms, but not of other starch-storing organs.

  2001cited by this paper
• Chloroplast transit peptides: structure, function and evolution.

  2000cited by this paper
• The Major Form of ADP-Glucose Pyrophosphorylase in Maize Endosperm Is Extra-Plastidial

  1996cited by this paper
• A single mutation that increases maize seed weight.

  1996cited by this paper
• A single gene encodes two different transcripts for the ADP-glucose pyrophosphorylase small subunit from barley (Hordeum vulgare).

  1996cited by this paper
• Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics.

  1984cited by this paper

• Polyploidization-Driven Functional Innovation of AGPase Small Subunit Gene APS1 Regulates Starch Biosynthesis in Banana (Musa acuminata).

  2026cites this paper
• The bHLH transcription factor PIL1 orchestrates starch synthesis in key cereal crops

  2025cites this paper
• ADP-glucose pyrophosphorylase gene family in soybean and implications in drought stress tolerance

  2024cites this paper
• TaDL interacts with TaB3 and TaNF‐YB1 to synergistically regulate the starch synthesis and grain quality in bread wheat

  2024cites this paper
• Pericarp starch metabolism is associated with caryopsis development and endosperm starch accumulation in common wheat.

  2023cites this paper
• Serine 31 Phosphorylation-Driven Regulation of AGPase Activity: Potential Implications for Enhanced Starch Yields in Crops

  2023cites this paper
• JAZ1 gene regulates starch biosynthesis and changes physicochemical properties in wheat grains

  2023cites this paper
• Growth and Carbohydrate Compositions of Three Gossypium Species Inoculated with Rhizophagus intraradices under Salinity Stress

  2023cites this paper
• Wheat E3 ubiquitin ligase TaGW2-6A degrades TaAGPS to affect seed size.

  2022cites this paper
• Functional analysis of the eTM-miR171-SCL6 module regulating somatic embryogenesis in Lilium pumilum DC. Fisch

  2022cites this paper
• Characterization of Various Subunit Combinations of ADP-Glucose Pyrophosphorylase in Duckweed (Landoltia punctata)

  2022cites this paper
• The 218th amino acid change of Ser to Ala in TaAGPS-7A increases enzyme activity and grain weight in bread wheat

  2022cites this paper
• Genetic variation in transcriptional regulation of wheat seed starch content and its conversion to bioethanol

  2021cites this paper
• Expression and regulation of genes involved in the reserve starch biosynthesis pathway in hexaploid wheat (Triticum aestivum L.)

  2020cites this paper
• A novel effect of glycine on the growth and starch biosynthesis of storage root in sweetpotato (Ipomoea batatas Lam.).

  2019cites this paper
• The synergistic effects of TaAGP.L-B1 and TaSSIVb-D mutations in wheat lead to alterations of gene expression patterns and starch content in grain development

  2019cites this paper
• Differential effects of synthetic media on long-term growth, starch accumulation and transcription of ADP-glucosepyrophosphorylase subunit genes in Landoltia punctata

  2019cites this paper
• A sucrose non‐fermenting‐1‐related protein kinase‐1 gene, IbSnRK1, improves starch content, composition, granule size, degree of crystallinity and gelatinization in transgenic sweet potato

  2018influential citation
• Non-Structural Carbohydrates Accumulation in Contrasting Rice Genotypes Subjected to High Night Temperatures

  2017cites this paper
• Phenylalanine Ammonia-Lyase and Source-Flow-Sink Related Attributes in Rice Genotypes Subjected to High Night Temperatures

  2017cites this paper