Development, characterization, and cross-amplification of polymorphic microsatellite markers for North American Trachymyrmex and Mycetomoellerius ants

The objective of this study is to develop and identify polymorphic microsatellite markers for fungus-gardening (attine) ants in the genus Trachymyrmex sensu lato. These ants are important ecosystem engineers and have been a model group for understanding complex symbiotic systems, but very little is understood about the intraspecific genetic patterns across most North American attine species. These microsatellite markers will help to better study intraspecific population genetic structure, gene flow, mating habits, and phylogeographic patterns in these species and potentially other congeners. Using next-generation sequencing techniques, we identified 17 and 12 polymorphic microsatellite markers from T. septentrionalis and Mycetomoellerius (formerly Trachymyrmex) turrifex, respectively, and assessed the genetic diversity of each marker. We also analyzed the cross-amplification success of the T. septentrionalis markers in two other closely related Trachymyrmex species, and identified 10 and 12 polymorphic markers for T. arizonensis and T. pomonae, respectively.

• Publication year

  2020

• Venue

  BMC Research Notes

• Publication date

  2020-01-27

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/s13104-020-05015-3PMID 32204727PMCID 7092486
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• High diversity and multiple invasions to North America by fungi grown by the northern-most Trachymyrmex and Mycetomoellerius ant species

  2020cited by this paper
• The molecular phylogenetics of Trachymyrmex Forel ants and their fungal cultivars provide insights into the origin and coevolutionary history of ‘higher‐attine’ ant agriculture

  2019cited by this paper
• Landscape genomics of an obligate mutualism: Concordant and discordant population structures between the leafcutter ant Atta texana and its two main fungal symbiont types

  2019cited by this paper
• Weak population structure in the ant Formica fusca

  2018cited by this paper
• Comparative assessment of SSR and SNP markers for inferring the population genetic structure of the common fungus Armillaria cepistipes

  2017cited by this paper
• Phylogenomic species delimitation and host‐symbiont coevolution in the fungus‐farming ant genus Sericomyrmex Mayr (Hymenoptera: Formicidae): ultraconserved elements (UCEs) resolve a recent radiation

  2017cited by this paper
• A comparative assessment of SNP and microsatellite markers for assigning parentage in a socially monogamous bird

  2017cited by this paper
• Variability in total project and per sample genotyping costs under varying study designs including with microsatellites or SNPs to answer conservation genetic questions

  2017cited by this paper
• Bioturbation by the Fungus-Gardening Ant, Trachymyrmex septentrionalis

  2016cited by this paper
• Phylogeography of the sand dune ant Mycetophylax simplex along the Brazilian Atlantic Forest coast: remarkably low mtDNA diversity and shallow population structure

  2015cited by this paper
• The Most Relictual Fungus-Farming Ant Species Cultivates the Most Recently Evolved and Highly Domesticated Fungal Symbiont Species

  2015cited by this paper
• Large-scale population genetics of the mountain ant Proformica longiseta (Hymenoptera: Formicidae)

  2015cited by this paper
• A comparison of single nucleotide polymorphism and microsatellite markers for analysis of parentage and kinship in a cooperatively breeding bird

  2015cited by this paper
• Gone to Texas: phylogeography of two Trachymyrmex (Hymenoptera: Formicidae) species along the southeastern coastal plain of North America

  2015cited by this paper
• Correction: Rapid Microsatellite Identification from Illumina Paired-End Genomic Sequencing in Two Birds and a Snake

  2015cited by this paper
• Variable interaction specificity and symbiont performance in Panamanian Trachymyrmex and Sericomyrmex fungus-growing ants

  2014cited by this paper
• Development of twenty-one polymorphic microsatellite markers for the fungus-growing ant, Mycocepurus goeldii (Formicidae: Attini), using Illumina paired-end genomic sequencing

  2014cited by this paper
• Co‐evolutionary patterns and diversification of ant–fungus associations in the asexual fungus‐farming ant Mycocepurus smithii in Panama

  2013cited by this paper
• Male-biased dispersal promotes large scale gene flow in a subterranean army ant, Dorylus (Typhlopone) fulvus

  2013cited by this paper
• Comparison of the effectiveness of microsatellites and SNP panels for genetic identification, traceability and assessment of parentage in an inbred Angus herd

  2013cited by this paper
• Microsatellite loci characterized in the leaf-cutter ant Atta laevigata

  2013cited by this paper
• Sex at the margins: parthenogenesis vs. facultative and obligate sex in a Neotropical ant

  2013cited by this paper
• Development and characterization of twenty-two polymorphic microsatellite markers for the leafcutter ant, Acromyrmexlundii, utilizing Illumina sequencing

  2013cited by this paper
• Dynamic Disease Management in Trachymyrmex Fungus-Growing Ants (Attini: Formicidae)

  2013cited by this paper
• Cyatta abscondita: Taxonomy, Evolution, and Natural History of a New Fungus-Farming Ant Genus from Brazil

  2013cited by this paper
• Rapid Microsatellite Identification from Illumina Paired-End Genomic Sequencing in Two Birds and a Snake

  2012cited by this paper
• GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update

  2012cited by this paper
• Primer3—new capabilities and interfaces

  2012cited by this paper
• Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data

  2012cited by this paper
• Phylogeography of the parthenogenic ant Platythyrea punctata: highly successful colonization of the West Indies by a poor disperser

  2011cited by this paper
• Cryptic sexual populations account for genetic diversity and ecological success in a widely distributed, asexual fungus-growing ant

  2011cited by this paper
• Distribution of the fungus‐gardening ant (Trachymyrmex septentrionalis) during and after a record drought

  2010cited by this paper
• Rapid identification of thousands of copperhead snake (Agkistrodon contortrix) microsatellite loci from modest amounts of 454 shotgun genome sequence

  2010cited by this paper
• Comparative performance of single nucleotide polymorphism and microsatellite markers for population genetic analysis.

  2009cited by this paper
• Fast, cost-effective development of species-specific microsatellite markers by genomic sequencing.

  2009cited by this paper
• Inferring ant evolution in the age of molecular data (Hymenoptera: Formicidae)

  2009cited by this paper
• Major evolutionary transitions in ant agriculture

  2008cited by this paper
• genepop’007: a complete re‐implementation of the genepop software for Windows and Linux

  2008cited by this paper
• Sequencing breakthroughs for genomic ecology and evolutionary biology

  2008cited by this paper
• Phylogeography of post‐Pleistocene population expansion in a fungus‐gardening ant and its microbial mutualists

  2008cited by this paper
• Microsatellite null alleles and estimation of population differentiation.

  2007cited by this paper
• A review of the North American species of the fungus-gardening ant genus Trachymyrmex (Hymenoptera: Formicidae)

  2007influential reference
• Enhancements and modifications of primer design program Primer3

  2007cited by this paper
• Nuclear mitochondrial‐like sequences in ants: evidence from Atta cephalotes (Formicidae: Attini)

  2007cited by this paper
• genalex 6: genetic analysis in Excel. Population genetic software for teaching and research

  2006cited by this paper
• Microsatellites: simple sequences with complex evolution

  2004cited by this paper
• Micro-Checker: Software for identifying and correcting genotyping errors in microsatellite data

  2004cited by this paper
• SNPs in ecology, evolution and conservation

  2004cited by this paper
• Patterns of population subdivision and gene flow in the ant Nothomyrmecia macrops reflected in microsatellite and mitochondrial DNA markers

  2003cited by this paper
• Publisher's Note

  2003cited by this paper
• Population genetic structure and male‐biased dispersal in the queenless ant Diacamma cyaneiventre

  2002cited by this paper
• Microsatellite primers for fungus‐growing ants

  2002cited by this paper
• An economic method for the fluorescent labeling of PCR fragments

  2000cited by this paper
• Polymorphic microsatellite DNA markers for the marine gastropod Littorina subrotundata

  2000cited by this paper
• Queen mating frequency and relatedness in young Atta sexdens colonies

  2000cited by this paper
• Evolutionary transition from single to multiple mating in fungus‐growing ants

  1999cited by this paper
• Multiple paternity in the leafcutter ant Atta colombica — a microsatellite DNA study

  1998cited by this paper

• Phylogeography of the imperiled Comanche Harvester Ant (Pogonomyrmex comanche)

  2024cites this paper
• Environments and Hosts Structure the Bacterial Microbiomes of Fungus-Gardening Ants and their Symbiotic Fungus Gardens

  2022cites this paper
• Male‐biased dispersal in a fungus‐gardening ant symbiosis

  2021influential citation
• A schistosoma mansoni tri- and tetramer microsatellite catalog for genetic population diversity and differentiation.

  2021cites this paper