The mechanism of sublethal chlorantraniliprole exposure causing silkworm pupation metamorphosis defects.

BACKGROUND Chlorantraniliprole (CAP) is widely used in agriculture and forestry to prevent and control pests. The effects of environmental CAP residue on non-target insect metamorphosis development have not been reported. Our research aimed to investigate the sublethal effect of CAP on larvae-pupae transformation process in silkworm, and also explore the mechanism of sublethal CAP exposure mediated pupation metamorphosis defects. RESULT Sublethal CAP exposure affected the growth and development of silkworm larvae and caused defects in pupation metamorphosis. After CAP exposure, the formation of prepupa procuticle, ecdysial membrane, and new epidermis was inhibited. Also, the level of 20-hydroxyecdysone (20E) and the mRNA levels of 20E signaling pathway related genes, EcR, USP, E74, E75 and Ftz-f1 were significantly reduced. Moreover, the genes involved in chitin synthesis, such as ChsA, CDA1 and CDA2, were down-regulated. Injection of 20E led to upregulation of the chitin synthesis-related genes and increased formation of new epidermis in CAP treated silkworm. However, the 20E injection failed to rescue the down-regulation of Ftz-f1 and the defects in pupation metamorphosis. CONCLUSION Our results suggested that 20E is a target hormone of CAP exposure mediated epidermis formation phenotype. The Ftz-f1 was silenced by CAP and it might be a direct target gene of sublethal CAP exposure. Our study provided new evidences about the effects of sublethal CAP exposure on insect development and metamorphosis. This article is protected by copyright. All rights reserved.

• Publication year

  2020

• Venue

  Pest Management Science

• Publication date

  2020-04-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1002/ps.5836PMID 32237032
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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