Immunomodulatory Effect of CpG ODN-Adjuvanted Bacterin Against Salmonella enterica serovar Enteritidis in Broiler Chickens.

Bacterial oligodeoxynucleotide containing Cytosine Guanine motifs (CpG-ODN) has been reported to induce immunostimulatory activity against a variety of bacterial, viral, and protozoan infections in a wide range of vertebrate species. The objective of this study was to investigate the dose-dependent immunomodulatory effect of CpG ODN on Salmonella Enteritidis bacterin in broiler chickens. Two hundreds one-day-old broiler chicks, divided into 5 groups, were used in this study. First three groups were immunized with Salmonella Enteritidis bacterin adjuvanted with different doses of CpG ODN (50μg, 100μg and 200μg). The control groups included a group that was immunized with Salmonella Enteritidis bacterin adjuvanted with aluminum hydroxide and a non-immunized group. The intestinal colonization, cellular responses, mucosal and systemic immune responses of immunized chickens was measured at different intervals, until 42 days of age. At two weeks post-immunization, 20 chicks from each group were orally challenged by Salmonella Enteritidis fresh bacterial culture (1.2x10 CFU/ml). The survival rates and the pathological changes of challenged chickens in the different groups were monitored for extra 10 days. Compared to the aluminum hydroxide adjuvanted bacterin, the CpG-ODN adjuvant bacterin induced significant protection and improved survival rate of challenged chickens. Also Salmonella Enteritidis was not recovered from the intestinal tract of vaccinated challenged groups. There was a significant dose-dependent immunostimulatory adjuvant effect of CPG-ODN on the level of secretory IgA and the induced mucosal responses. The 200-CpG ODN group showed the highest IgA response followed by 100-CpG ODN group then the 50-CpG ODN and the aluminum hydroxide groups (P < 0.05). Also, cellular interactions were remarkably reduced in the liver and intestine of CpG ODN-treated chickens. No inflammatory cellular infiltrations were seen in the liver and intestine of 200-CpG ODN treated group. In conclusion, the presented findings have shown the significant immunostimulatory effect of CpGODN and its effect on Salmonella Enteritidis bacterin in controlling Salmonella infection in broiler chickens.

• Publication year

  2020

• Venue

  Unknown venue

• Publication date

  2020-03-25

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.36380/scil.2020.wvj8
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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  2019influential reference
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  2017influential reference
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• [Food borne infectious outbreaks, Austria 2005].

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• Innate immune recognition.

  2002influential reference
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  2002influential reference

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