Effect of salinity acclimation on osmoregulation, oxidative stress, and metabolic enzymes in the invasive Xenopus laevis.

Aquatic animals often display physiological adjustments to improve their biological performance and hydrosaline balance in saline environments. In addition to energetic costs associated with osmoregulation, oxidative stress, and the activation of the antioxidant system are common cellular responses to salt stress in many species, but the knowledge of osmoregulation-linked oxidative homeostasis in amphibians is scarce. Here we studied the biochemical responses and oxidative responses of Xenopus laevis females exposed for 40 days to two contrasting salinities: hypo-osmotic (150 mOsm·kg-1 ·H2 O NaCl, HYPO group) and hyper-osmotic environments (340 mOsm·kg-1 ·H2 O NaCl, HYPER group). We found an increase of plasma osmolality and plasma urea concentration in the animals incubated in the HYPER treatment. Increases in electrolyte concentration were paralleled with an increase of both citrate synthase and cytochrome c oxidase activities in liver and heart. Interestingly, HYPO group had higher catabolic activity of the skin and liver total antioxidant capacity (TAC), compared with animals from the HYPER group. Moreover, there was an inverse relationship between liver TAC and plasma osmolality; and with the metabolic enzymes from liver. These findings suggest that salinity induces changes in urea metabolism and specific activity of metabolic enzymes, which appears to be tissue-dependent in X. laevis. Contrary to our expectations, we also found a moderate change in the oxidative status as revealed by the increase in TAC activity in the animals acclimated to low salinity medium, but constancy in the lipid peroxidation of membranes.

• Publication year

  2020

• Venue

  Journal of Experimental Zoology Part A Ecological and Integrative Physiology

• Publication date

  2020-04-19

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1002/jez.2360PMID 32306529
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The interplay between ambient temperature and salt intake affects oxidative status and immune responses in a ubiquitous Neotropical passerine, the rufous-collared sparrow.

  2019cited by this paper
• Effects of temperature and salinity on body fluid dynamics and metabolism in the estuarine diamondback terrapin (Malaclemys terrapin)

  2019cited by this paper
• Twenty years of the 'Preparation for Oxidative Stress' (POS) theory: Ecophysiological advantages and molecular strategies.

  2019cited by this paper
• Antioxidant responses to salinity stress in an invasive species, the red-eared slider (Trachemys scripta elegans) and involvement of a TOR-Nrf2 signaling pathway.

  2019cited by this paper
• Synergistic effects of temperature and salinity on the gene expression and physiology of Crassostrea virginica.

  2019cited by this paper
• Terrestrial birds in coastal environments: metabolic rate and oxidative status varies with the use of marine resources

  2018cited by this paper
• Effects of ocean acidification and salinity variations on the physiology of osmoregulating and osmoconforming crustaceans

  2018cited by this paper
• Mitochondrial Adaptations to Variable Environments and Their Role in Animals' Stress Tolerance.

  2018cited by this paper
• Cellular metabolism and oxidative stress as a possible determinant for longevity in small breed and large breed dogs

  2018cited by this paper
• Multimarker response to salinity stress in two estuarine bivalves of different genetic diversity: Mya arenaria and Limecola balthica from the Gulf of Gdańsk (southern Baltic Sea)

  2018cited by this paper
• Behavioral and Physiological Responses of an Amphibious, Euryhaline Mangrove Fish to Acute Salinity Exposure

  2018cited by this paper
• Osmoregulation, bioenergetics and oxidative stress in coastal marine invertebrates: raising the questions for future research

  2017cited by this paper
• Adaptive responses to salinity stress across multiple life stages in anuran amphibians

  2017cited by this paper
• Coping with Salt Water Habitats: Metabolic and Oxidative Responses to Salt Intake in the Rufous-Collared Sparrow

  2017cited by this paper
• Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?

  2016cited by this paper
• Native and introduced clams biochemical responses to salinity and pH changes.

  2016cited by this paper
• Metabolic cost of osmoregulation in a hypertonic environment in the invasive African clawed frog Xenopus laevis

  2016cited by this paper
• Hypersalinity drives physiological and morphological changes in Limia perugiae (Poeciliidae)

  2016cited by this paper
• Occurrence of Amphibians in Saline Habitats: A Review and Evolutionary Perspective

  2015cited by this paper
• Effects of Low Salinity on Adult Behavior and Larval Performance in the Intertidal Gastropod Crepipatella peruviana (Calyptraeidae)

  2014cited by this paper
• Oxidative stress as a cost of reproduction: Beyond the simplistic trade‐off model

  2014cited by this paper
• Osmoregulatory and metabolic costs of salt excretion in the Rufous-collared sparrow Zonotrichia capensis.

  2013cited by this paper
• Oxidative damage, ageing, and life-history evolution: where now?

  2012cited by this paper
• OXIDATIVE DAMAGE INCREASES WITH REPRODUCTIVE ENERGY EXPENDITURE AND IS REDUCED BY FOOD‐SUPPLEMENTATION

  2012cited by this paper
• Assessment of mitochondrial respiratory chain enzymatic activities on tissues and cultured cells

  2012cited by this paper
• Larval Tolerance to Salinity in Three Species of Australian Anuran: An Indication of Saline Specialisation in Litoria aurea

  2012cited by this paper
• Understanding the energetic costs of living in saline environments: effects of salinity on basal metabolic rate, body mass and daily energy consumption of a long-distance migratory shorebird

  2011cited by this paper
• Responses of free radical metabolism to air exposure or salinity stress, in crabs (Callinectes danae and C. ornatus) with different estuarine distributions.

  2011cited by this paper
• Has the Earth’s sixth mass extinction already arrived?

  2011cited by this paper
• Climate change impacts on water salinity and health

  2011cited by this paper
• Telomeres and life histories: the long and the short of it

  2010cited by this paper
• Profiles of antioxidant gene expression and physiological changes by thermal and hypoosmotic stresses in black porgy (Acanthopagrus schlegeli).

  2010cited by this paper
• Osmoregulatory and demographic responses to salinity of the exotic cladoceran Daphnia exilis

  2010cited by this paper
• The lateral intercellular space as osmotic coupling compartment in isotonic transport

  2009cited by this paper
• Oxidative stress as a mediator of life history trade-offs: mechanisms, measurements and interpretation.

  2009cited by this paper
• Physiology of cell volume regulation in vertebrates.

  2009cited by this paper
• Stress, Metabolism, and Antioxidants in Two Wild Passerine Bird Species

  2008cited by this paper
• Habitat Split and the Global Decline of Amphibians

  2007cited by this paper
• Effects of environmental salinity and temperature on osmoregulatory ability, organic osmolytes, and plasma hormone profiles in the Mozambique tilapia (Oreochromis mossambicus).

  2007cited by this paper
• Immune activation increases susceptibility to oxidative tissue damage in Zebra Finches

  2006cited by this paper
• Effects of NaCl salinity on the population dynamics of freshwater zooplankton (rotifers and cladocerans)

  2006cited by this paper
• Mechanisms of hyperosmotic acclimation in Xenopus laevis (salt, urea or mannitol)

  2004cited by this paper
• Birds sacrifice oxidative protection for reproduction

  2004cited by this paper
• Global amphibian declines: sorting the hypotheses

  2003cited by this paper
• Cell volume regulation: osmolytes, osmolyte transport, and signal transduction.

  2003cited by this paper
• PREDATOR CUES AND PESTICIDES: A DOUBLE DOSE OF DANGER FOR AMPHIBIANS

  2003cited by this paper
• Consequences of climate-induced salinity increases on zooplankton abundance and diversity in coastal lakes

  2003cited by this paper
• Proton pump-driven cutaneous chloride uptake in anuran amphibia.

  2003cited by this paper
• BIOTIC INVASIONS: CAUSES, EPIDEMIOLOGY, GLOBAL CONSEQUENCES, AND CONTROL

  2000cited by this paper
• Urea-associated oxidative stress and Gadd153/CHOP induction.

  1999cited by this paper
• Novel cardiac protective effects of urea: from shark to rat

  1999cited by this paper
• Antioxidant defenses and lipid peroxidation damage in estivating toads, Scaphiopus couchii

  1998cited by this paper
• Urea and amphibian water economy.

  1997cited by this paper
• Beyond Global Warming: Ecology and Global Change

  1994cited by this paper
• IN VITRO METABOLIC DEPRESSION OF TISSUES FROM THE AESTIVATING FROG NEOBATRACHUS PELOBATOIDES

  1991cited by this paper
• Osmoregulation of the african clawed frog. Xenopus laevis, in hypersaline media

  1976cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Accelerated synthesis of urea in Xenopus laevis during osmotic stress.

  1970cited by this paper
• Ornithine-Urea Cycle Activity in Xenopus laevis: Adaptation in Saline

  1967cited by this paper
• Nasal Salt Excretion and the Possible Function of the Cloaca in Water Conservation

  1963cited by this paper
• Oxygen consumption and active sodium transport in the isolated and short-circuited frog skin.

  1956cited by this paper
• Oxygen Consumption and Active Sodium Transport in the Isolated and Short‐Circuited Frog Skin1

  1956cited by this paper

• Effects of body mass and legacy of pesticide contamination on oxidative stress biomarkers in larval Rana sylvatica under baseline and NaCl-contaminated conditions.

  2025cites this paper
• Physiological and behavioral responses to novel saline conditions in an invasive treefrog.

  2024cites this paper
• Integrated analysis of transcriptome and metabolome reveals chronic low salinity stress responses in the muscle of Exopalaemon carinicauda.

  2024cites this paper
• Physiological Responses of Juvenile Bullfrogs (Aquarana catesbeiana) to Salinity Stress

  2024cites this paper
• Anthropization, Salinity and Oxidative Stress in Animals in the Coastal Zone

  2023cites this paper
• Biochemical and osmoregulatory responses of the African clawed frog experimentally exposed to salt and pesticide.

  2022cites this paper