Structure of the Type IX Group B Streptococcus Capsular Polysaccharide and Its Evolutionary Relationship with Types V and VII

Background: The capsule of Group B Streptococcus is an important virulence factor and vaccine target. Results: Analysis of type IX polysaccharide revealed a structure similar to type V and VII structures. Conclusion: The structural and phylogenetic basis for the differentiation between types V, VII, and IX was elucidated. Significance: Determination of the type IX structure is instrumental for the development of a carbohydrate-based vaccine. The Group B Streptococcus capsular polysaccharide type IX was isolated and purified, and the structure of its repeating unit was determined. Type IX capsule →4)[NeupNAc-α-(2→3)-Galp-β-(1→4)-GlcpNAc-β-(1→6)]-β-GlcpNAc-(1→4)-β-Galp-(1→4)-β-Glcp-(1→ appears most similar to types VII and V, although it contains two GlcpNAc residues. Genetic analysis identified differences in cpsM, cpsO, and cpsI gene sequences as responsible for the differentiation between the three capsular polysaccharide types, leading us to hypothesize that type V emerged from a recombination event in a type IX background.

• Publication year

  2014

• Venue

  Journal of Biological Chemistry

• Publication date

  2014-07-02

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.M114.567974PMID 24990951PMCID PMC4156066
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Type V is hypothesized to have emerged from a recombination event in a type IX background.

  Confidence 0.77

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review
• Differences in cpsM, cpsO, and cpsI gene sequences account for the differentiation among capsular polysaccharide types V, VII, and IX.

  Confidence 0.93

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review
• The type IX polysaccharide is structurally most similar to the type V and type VII capsules, despite containing two GlcNAc residues.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review
• The repeating-unit structure of the Group B Streptococcus capsular polysaccharide type IX was determined after the capsule was isolated and purified.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review

• cpsi

  gene, gene sequence

  A capsule biosynthesis gene locus examined in the comparative sequence analysis of the capsule types.

  Aliases: cpsI gene

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review
• cpsm

  gene, gene sequence

  A capsule biosynthesis gene locus examined in the comparative sequence analysis of the capsule types.

  Aliases: cpsM gene

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review
• cpso

  gene, gene sequence

  A capsule biosynthesis gene locus examined in the comparative sequence analysis of the capsule types.

  Aliases: cpsO gene

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review
• glcnac residues

  monosaccharide, sugar residue

  N-acetylglucosamine sugar units present within the polysaccharide repeat unit.

  Aliases: GlcNAc, N-acetylglucosamine residues

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review
• group b streptococcus capsular polysaccharide type ix

  capsular polysaccharide, biological molecule

  The serotype IX capsular polysaccharide from Group B Streptococcus that is the molecule analyzed in the paper.

  Aliases: GBS type IX capsule, serotype IX

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review
• recombination event

  evolutionary mechanism, genetic event

  An exchange of genetic material inferred as the evolutionary mechanism shaping the capsule locus background.

  Aliases: genetic recombination

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review
• repeating unit structure

  molecular structure

  The arrangement of sugar residues and glycosidic linkages that defines one repeat of the polysaccharide chain.

  Aliases: repeat-unit structure

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review
• type v capsular polysaccharide

  capsular polysaccharide, biological molecule

  The serotype V Group B Streptococcus capsule used as a comparator in the structural and evolutionary discussion.

  Aliases: GBS type V capsule, serotype V

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review
• type vii capsular polysaccharide

  capsular polysaccharide, biological molecule

  The serotype VII Group B Streptococcus capsule used as a comparator in the structural and evolutionary discussion.

  Aliases: GBS type VII capsule, serotype VII

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) reviewAK (4715169a40) review

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