In vivo and in vitro effect of imipramine and fluoxetine on Na+,K+-ATPase activity in synaptic plasma membranes from the cerebral cortex of rats.

The effects of in vivo chronic treatment and in vitro addition of imipramine, a tricyclic antidepressant, or fluoxetine, a selective serotonin reuptake inhibitor, on the cortical membrane-bound Na+,K+-ATPase activity were studied. Adult Wistar rats received daily intraperitoneal injections of 10 mg/kg of imipramine or fluoxetine for 14 days. Twelve hours after the last injection rats were decapitated and synaptic plasma membranes (SPM) from cerebral cortex were prepared to determine Na+,K+-ATPase activity. There was a significant decrease (10%) in enzyme activity after imipramine but fluoxetine treatment caused a significant increase (27%) in Na+,K+-ATPase activity compared to control (P<0.05, ANOVA; N = 7 for each group). When assayed in vitro, the addition of both drugs to SPM of naive rats caused a dose-dependent decrease in enzyme activity, with the maximal inhibition (60-80%) occurring at 0.5 mM. We suggest that a) imipramine might decrease Na+,K+-ATPase activity by altering membrane fluidity, as previously proposed, and b) stimulation of this enzyme might contribute to the therapeutic efficacy of fluoxetine, since brain Na+,K+-ATPase activity is decreased in bipolar patients.

• Publication year

  2001

• Venue

  Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

• Publication date

  2001-10-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1590/S0100-879X2001001000005PMID 11593300
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Pharmacological treatment of depression.

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  1995cited by this paper
• The Na,K-ATPase hypothesis for bipolar illness.

  1995cited by this paper
• Alanine reverses the inhibitory effect of phenylalanine and its metabolites on Na+,K(+)-ATPase in synaptic plasma membranes from cerebral cortex of rats.

  1995cited by this paper
• Ions and energy in mammalian brain.

  1994cited by this paper
• Interaction of tricyclic drug analogs with synaptic plasma membranes: structure-mechanism relationships in inhibition of neuronal Na+/K(+)-ATPase activity.

  1993influential reference
• Regional brain expression of serotonin transporter mRNA and its regulation by reuptake inhibiting antidepressants.

  1993cited by this paper
• Abnormal Platelet Aggregation Associated with Fluoxetine Therapy

  1992cited by this paper
• Regional Distribution of Imipramine, Desipramine and Specific [3H]Desipramine Binding Sites in the Rat Brain after Acute and Chronic Treatment with Imipramine

  1991cited by this paper
• cAMP binding proteins in the rat cerebral cortex after administration of selective 5-HT and NE reuptake blockers with antidepressant activity.

  1991cited by this paper
• The pharmacologic treatment of depression.

  1991cited by this paper
• Monoamines and Abnormal Behaviour a Multi-Aminergic Perspective

  1990cited by this paper
• Imipramine alters β-adrenergic, but not serotonergic, mediated responses in rat hippocampal pyramidal cells

  1989cited by this paper
• Imipramine alters beta-adrenergic, but not serotonergic, mediated responses in rat hippocampal pyramidal cells.

  1989cited by this paper
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  1989cited by this paper
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  1989cited by this paper
• Clinical Pharmacology and Pharmacokinetics of Fluoxetine: A Review

  1988cited by this paper
• Effects of chlorpromazine and imipramine on rat heart subcellular membranes.

  1980cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• IMIPRAMINE‐INDUCED CHANGES OF BRAIN ADENOSINE TRIPHOSPHATASE ACTIVITY

  1973cited by this paper

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