A novel peptide-based fluorescent probe for sensitive detection of zinc (II) and its applicability in live cell imaging.

In this work, we report SPSS synthesis of a new peptide-based fluorescent probe (L) capable of detecting Zn2+ with little interference in 100% aqueous solutions at physiological pH. Furthermore, L showed excellent sensitivity, with a detection limit of 26.77 nM. The 2:1 binding ratio between L and Zn2+ was determined using fluorometric titration, Job's plot and ESI-MS analyses. The "off-on-off" type fluorescence change of L was demonstrated by alternately adding Zn2+ and EDTA based on a formation-separation process of the complex, indicating that L could serve as a reversible probe. Moreover, MTT studies demonstrated that L has low biotoxicity, and could be successfully used for detection of Zn2+ and EDTA in live cells.

• Publication year

  2020

• Venue

  Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy

• Publication date

  2020-06-02

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.saa.2020.118549PMID 32526399
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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