Proteomic analysis reveals the mechanisms involved in the enhanced biocontrol efficacy of Rhodotorula mucilaginosa induced by chitosan

Abstract The biocontrol efficacy of the yeast, Rhodotorula mucilaginosa against postharvest diseases of strawberries can be significantly enhanced by culturing the yeast in a medium containing chitosan. The molecular mechanisms involved in the enhanced biocontrol efficacy of R. mucilaginosa, however, have not been comprehensively explored. Results of the present study indicate that the biocontrol efficacy of the yeast against gray mold (Botrytis cinerea) of strawberry was greatest when the yeast was cultured in a medium containing 0.5% (w:v) chitosan for 24 h before use. Proteomic and bioinformatic analyses revealed that proteins involved in growth and reproduction, energy metabolism, antioxidant response, response to abiotic stress, and degradation of pathogen cell walls were all up-regulated by chitosan resulting in the enhanced antagonistic activity of R. mucilaginosa against B. cinerea. The decrease in ROS accumulation in R. mucilaginosa cultured in a medium containing chitosan was in agreement with the increased abundance of proteins related to antioxidant defense. Key differentially expressed proteins (DEPs) were identified and 12 randomly-selected DEPs were validated by parallel reaction monitoring. The present results provide a theoretical reference for further study of the molecular mechanisms involved in the improvement of biocontrol efficacy of antagonistic yeast by elicitor molecules and also provide a novel approach for improving biological control of postharvest diseases.

• Publication year

  2020

• Venue

  Biological control (Print)

• Publication date

  2020-10-01

• Fields of study

  Biology, Materials Science, Environmental Science

• Identifiers
  DOI 10.1016/j.biocontrol.2020.104325
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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