Alternative primers to identify a range of apicomplexan parasites.

S. N. Pinheiro,M. F. B. de Souza,C. B. S. Oliveira,V. F. A. Andrade Neto,D. C. F. Lanza

Published 2020 in Journal of Microbiological Methods

ABSTRACT

Here we present an alternative polymerase chain reaction (PCR) approach using 18S rDNA to identify apicomplexan parasites. A new primer set was designed and evaluated in silico and in vitro. This new PCR could detect some apicomplexan genera based on amplicon size and identify at least 45 species after sequencing.

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