Detection and characterization of mammalian DNA polymerase beta mutants by functional complementation in Escherichia coli.

No abstract is available for this paper.

• Publication year

  1993

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  1993-05-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1073/PNAS.90.10.4626PMID 8506308PMCID PMC46565
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Structure-function analysis of DNA polymerase-beta using monoclonal antibodies: identification of a putative nucleotide binding domain.

  1992cited by this paper
• Mammalian DNA polymerase beta can substitute for DNA polymerase I during DNA replication in Escherichia coli.

  1992cited by this paper
• Overproduction of DnaE protein (alpha subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I

  1992cited by this paper
• Mammalian DNA polymerase beta: characterization of a 16-kDa transdomain fragment containing the nucleic acid-binding activities of the native enzyme.

  1992cited by this paper
• DNA polymerase beta and DNA synthesis in Xenopus oocytes and in a nuclear extract.

  1992cited by this paper
• Aspartic acid residues at positions 190 and 192 of rat DNA polymerase beta are involved in primer binding.

  1991cited by this paper
• PCR-mediated chemical mutagenesis of cloned duplex DNAs.

  1991cited by this paper
• Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli.

  1990cited by this paper
• Site-directed mutagenesis of recombinant rat DNA polymerase beta: involvement of arginine-183 in primer recognition.

  1990cited by this paper
• Aphidicolin hypersensitive mutant of chinese hamster V79 fibroblasts that underproduces DNA polymerase-α antigen

  1989cited by this paper
• Photoaffinity labeling of terminal deoxynucleotidyl transferase. 2. Identification of peptides in the nucleotide binding domain.

  1989cited by this paper
• Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme.

  1988cited by this paper
• High-copy-number and low-copy-number plasmid vectors for lacZ alpha-complementation and chloramphenicol- or kanamycin-resistance selection.

  1987cited by this paper
• Characterization of a temperature-sensitive mutant of mouse FM3A cells defective in DNA replication.

  1985cited by this paper
• Chromogenic immunodetection of human serum albumin and alpha-L-fucosidase clones in a human hepatoma cDNA expression library.

  1984cited by this paper
• Studies on transformation of Escherichia coli with plasmids.

  1983cited by this paper
• Detection of the catalytic activities of DNA polymerases and their associated exonucleases following SDS-polyacrylamide gel electrophoresis.

  1981cited by this paper
• Effects of different alleles of the E. coli K12 polA gene on the replication of non-transferring plasmids

  1976cited by this paper
• The transition between different physiological states during balanced growth of Salmonella typhimurium.

  1958cited by this paper
• Acetylornithinase of Escherichia coli: partial purification and some properties.

  1956cited by this paper

• Incompatibility and Interchangeability in Molecular Evolution

  2022cites this paper
• Triple gene expressions in yeast, Escherichia coli, and mammalian cells by transferring DNA fragments amplified from a mother yeast expression plasmid.

  2022cites this paper
• Identification and Characterization of Functional Biomolecules by In Vitro Selection

  2015cites this paper
• Cellular Roles of DNA Polymerase Beta

  2013cites this paper
• Generation and characterization of new highly thermostable and processive M-MuLV reverse transcriptase variants.

  2012cites this paper
• Antimutator variants of DNA polymerases

  2011cites this paper
• Toward Safe Genetically Modified Organisms through the Chemical Diversification of Nucleic Acids

  2009cites this paper
• A systematic review of studies comparing diagnostic clinical prediction rules with clinical judgement

  2009cites this paper
• Polymerase engineering: towards the encoded synthesis of unnatural biopolymers.

  2009cites this paper
• Compartmentalized self-replication: a novel method for the directed evolution of polymerases and other enzymes.

  2007cites this paper
• Mammary carcinogenesis in transgenic mice expressing a dominant-negative mutant of DNA polymerase beta in their mammary glands.

  2007cites this paper
• Polymorphisms of the DNA polymerase beta gene in breast cancer.

  2007cites this paper
• Polymorphisms of the DNA polymerase β gene in breast cancer

  2007cites this paper
• CELL-FREE SELECTION OF DNA-BINDING PROTEINS FOR FUTURE GENE THERAPY APPLICATIONS ∗

  2007cites this paper
• Neue Enzymeigenschaften durch gerichtete Evolution : Entwicklung und Charakterisierung einer thermostabilen Reversen Transkriptase aus einer DNA-abhängigen DNA-Polymerase

  2007cites this paper
• The evolution of DNA polymerases with novel activities.

  2005cites this paper
• Directed Evolution of Enzymes

  2005cites this paper
• A Novel Nuclear Protein, MGC5306 Interacts with DNA Polymerase β and Has a Potential Role in Cellular Phenotype

  2004cites this paper
• Use of Pol I-deficient E. coli for functional complementation of DNA polymerase.

  2003cites this paper
• Analysis of alterations in a base-excision repair gene in lung cancer.

  2003cites this paper
• Analysis of the DNA joining repertoire of Chlorella virus DNA ligase and a new crystal structure of the ligase-adenylate intermediate.

  2003cites this paper
• Research Report In Vitro Production and Screening of DNA Polymerase η Mutants for Catalytic Diversity

  2002cites this paper
• Threonine 79 Is a Hinge Residue That Governs the Fidelity of DNA Polymerase β by Helping to Position the DNA within the Active Site*

  2002cites this paper
• Heterogeneity in expression of DNA polymerase beta and DNA repair activity in human tumor cell lines.

  2002cites this paper
• In vitro production and screening of DNA polymerase eta mutants for catalytic diversity.

  2002cites this paper
• Rat DNA polymerase beta substitutes the repairing activity of DNA polymerase I in the lethal effect of UV light.

  2002cites this paper
• Structure-based combinatorial protein engineering (SCOPE).

  2002cites this paper
• A novel role of XRCC1 in the functions of a DNA polymerase beta variant.

  2001cites this paper
• Enzymatic properties of rat DNA polymerase beta mutants obtained by randomized mutagenesis.

  2001cites this paper
• Impaired repair activity of a truncated DNA polymerase beta protein.

  2001cites this paper
• DNA polymerases as engines for biotechnology.

  2001cites this paper
• DNA polymerase active site is highly mutable: evolutionary consequences.

  2000cites this paper
• A set of temperature sensitive-replication/-segregation and temperature resistant plasmid vectors with different copy numbers and in an isogenic background (chloramphenicol, kanamycin, lacZ, repA, par, polA).

  2000cites this paper
• Base excision repair in yeast and mammals.

  2000cites this paper
• Yeast and human genes that affect the Escherichia coli SOS response.

  1999cites this paper
• Improving enzymes for cancer gene therapy

  1999cites this paper
• Variant Forms of DNA Polymerase beta in Primary Lung Carcinomas

  1999cites this paper
• Involvement of phenylalanine 272 of DNA polymerase beta in discriminating between correct and incorrect deoxynucleoside triphosphates.

  1999cites this paper
• One small StEP in molecular evolution…

  1998cites this paper
• A genetic system to identify DNA polymerase beta mutator mutants.

  1997cites this paper
• A genetic system to identify DNA polymerase β mutator mutants

  1997cites this paper
• Creating novel enzymes by applied molecular evolution.

  1997cites this paper
• Principles and methods of evolutionary biotechnology.

  1997cites this paper
• Identification of novel mRNA isoforms for human DNA polymerase beta.

  1996cites this paper
• DNA End Joining by the Klenow Fragment of DNA Polymerase I*

  1996cites this paper
• Dominant negative rat DNA polymerase beta mutants interfere with base excision repair in Saccharomyces cerevisiae

  1996cites this paper
• Identification of Novel mRNA Isoforms for Human DNA Polymerase β

  1996cites this paper
• DNA repair functions in heterologous cells.

  1996cites this paper
• Unnatural nucleotide sequences in biopharmaceutics.

  1996cites this paper
• Random mutagenesis of Thermus aquaticus DNA polymerase I: concordance of immutable sites in vivo with the crystal structure.

  1996cites this paper
• Mutations in DNA-polymerase-Beta occur in breast, prostate and colorectal tumors.

  1995cites this paper
• DNA polymerase beta can substitute for DNA polymerase I in the initiation of plasmid DNA replication

  1995cites this paper
• Human immunodeficiency virus reverse transcriptase substitutes for DNA polymerase I in Escherichia coli.

  1995cites this paper
• A screen in Escherichia coli for nucleoside analogs that target human immunodeficiency virus (HIV) reverse transcriptase: coexpression of HIV reverse transcriptase and herpes simplex virus thymidine kinase

  1995cites this paper
• Characterization of DNA polymeraseβ mutants with amino acid substitutions located in the C-terminal portion of the enzyme

  1995cites this paper
• Microsatellite instabilities at five chromosomes in primary breast tumors.

  1994cites this paper
• DNA polymerase delta is required for base excision repair of DNA methylation damage in Saccharomyces cerevisiae.

  1994cites this paper
• Dinucleotide repeat polymorphisms at nine loci in sporadic colorectal cancer.

  1994cites this paper
• DNA polymerase delta is involved in the cellular response to UV damage in human cells.

  1994cites this paper
• Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase.

  1994cites this paper
• Mammalian gene studies

  1993cites this paper
• Multi-stage proofreading in DNA replication

  1993cites this paper
• Digital Commons @ New Haven Digital Commons @ New Haven

  year unknowncites this paper