Phage display technology for target determination of small-molecule therapeutics: an update

ABSTRACT Introduction Our understanding of the mechanism of action of bioactive small molecules contributes to the research and development of new medical drugs, as well as elucidating the pathological mechanisms underlying various diseases. Researchers in this field are committed to a very ambitious goal: the discovery of novel therapeutic compounds along with their molecular targets. To achieve this goal, new methodological developments are indispensable. Areas covered This review gives an update on the advancements of phage display (PD) technology in the past decade (2011–2020) for determining the targets of the small molecule therapeutics. In particular, other than providing a brief overview of this field of research, we focus on reporting the research trends and the results solely obtained using this strategy. Expert opinion Despite the development of bioinformatics tools and artificial intelligence (AI)-mediated methods, affinity-guided information obtained experimentally are still indispensable to identify drug-protein interactions. By taking advantage of small-molecule-oriented PD methods and their improvements, the extension of the druggable proteome will be further expanded, providing new opportunities to generate small-molecule therapeutics.

• Publication year

  2020

• Venue

  Expert Opinion on Drug Discovery

• Publication date

  2020-07-13

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1080/17460441.2020.1790523PMID 32660284
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Ribokinase screened from T7 phage displayed Mycobacterium tuberculosis genomic DNA library had good potential for the serodiagnosis of tuberculosis

  2019cited by this paper
• Phage Display Libraries: From Binders to Targeted Drug Delivery and Human Therapeutics

  2019cited by this paper
• Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules

  2019cited by this paper
• Reverse Chemical Proteomics Identifies an Unanticipated Human Target of the Antimalarial Artesunate.

  2019cited by this paper
• Chloramphenicol inhibits eukaryotic Ser/Thr phosphatase and infection-specific cell differentiation in the rice blast fungus

  2019cited by this paper
• Phage Display: Simple Evolution in a Petri Dish (Nobel Lecture).

  2019cited by this paper
• Isolation and characterization of malaria PfHRP2 specific VNAR antibody fragments from immunized shark phage display library

  2018cited by this paper
• A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning.

  2018cited by this paper
• 11-Aza-artemisinin Derivatives Exhibit Anticancer Activities by Targeting the Fatty Acid Binding Protein 6 (FABP6)

  2018cited by this paper
• Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule.

  2018cited by this paper
• A Multimodal Molecular Imaging Study Evaluates Pharmacological Alteration of the Tumor Microenvironment to Improve Radiation Response.

  2018cited by this paper
• Identification of proteins that bind to the neuroprotective agent neoechinulin A

  2018cited by this paper
• Combinatorial chemistry in drug discovery.

  2017cited by this paper
• Application of NMR to studies of intrinsically disordered proteins.

  2017cited by this paper
• Daptomycin, a last-resort antibiotic, binds ribosomal protein S19 in humans

  2017cited by this paper
• Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes

  2017cited by this paper
• Identification and Characterization of Strychnine-Binding Peptides Using Phage-Display Screening.

  2017cited by this paper
• Virus-Derived Peptides for Clinical Applications.

  2017cited by this paper
• Flavonoids: an overview

  2016cited by this paper
• Identification of UQCRB as an oxymatrine recognizing protein using a T7 phage display screen.

  2016cited by this paper
• Rapid Discovery of Functional Small Molecule Ligands against Proteomic Targets through Library-Against-Library Screening

  2016cited by this paper
• Simvastatin Sodium Salt and Fluvastatin Interact with Human Gap Junction Gamma-3 Protein

  2016cited by this paper
• Identifying the cellular targets of natural products using T7 phage display.

  2016cited by this paper
• Nature nurtures the design of new semi-synthetic macrolide antibiotics

  2016cited by this paper
• Natural Products as Sources of New Drugs from 1981 to 2014.

  2016cited by this paper
• A comprehensive map of molecular drug targets

  2016cited by this paper
• Allostery: An Overview of Its History, Concepts, Methods, and Applications

  2016cited by this paper
• HIF-1α suppressing small molecule, LW6, inhibits cancer cell growth by binding to calcineurin b homologous protein 1.

  2015cited by this paper
• Development of a T7 Phage Display Library to Detect Sarcoidosis and Tuberculosis by a Panel of Novel Antigens

  2015cited by this paper
• Sensitivity-enhanced NMR reveals alterations in protein structure by cellular milieus.

  2015cited by this paper
• Haem-activated promiscuous targeting of artemisinin in Plasmodium falciparum

  2015cited by this paper
• Multimodal biopanning of T7 phage-displayed peptides reveals angiomotin as a potential receptor of the anti-angiogenic macrolide Roxithromycin.

  2015cited by this paper
• The Small Molecule R-(-)-β-O-Methylsynephrine Binds to Nucleoporin 153 kDa and Inhibits Angiogenesis

  2015cited by this paper
• Scalable high throughput selection from phage-displayed synthetic antibody libraries.

  2015cited by this paper
• Novel anticancer agent, SQAP, binds to focal adhesion kinase and modulates its activity

  2015cited by this paper
• Ridaifen G, tamoxifen analog, is a potent anticancer drug working through a combinatorial association with multiple cellular factors.

  2015cited by this paper
• Marine spongian sesquiterpene phenols, dictyoceratin-C and smenospondiol, display hypoxia-selective growth inhibition against cancer cells.

  2014cited by this paper
• Combinatorial peptide libraries: mining for cell-binding peptides.

  2014cited by this paper
• EpsinR, a target for pyrenocine B, role in endogenous MHC‐II‐restricted antigen presentation

  2014cited by this paper
• Allostery in disease and in drug discovery.

  2013cited by this paper
• Total synthesis and anti-hepatitis C virus activity of MA026.

  2013cited by this paper
• Detection of hepatitis B virus core antigen by phage display mediated TaqMan real-time immuno-PCR.

  2013cited by this paper
• Molecular basis for the action of a dietary flavonoid revealed by the comprehensive identification of apigenin human targets

  2013cited by this paper
• Mapping a Disordered Portion of the Brz2001-Binding Site on a Plant Monooxygenase, DWARF4, Using a Quartz-Crystal Microbalance Biosensor-Based T7 Phage Display

  2013cited by this paper
• A chemical genomics approach to identification of interactions between bioactive molecules and alternative reading frame proteins.

  2013cited by this paper
• Ridaifen B, a tamoxifen derivative, directly binds to Grb10 interacting GYF protein 2.

  2013cited by this paper
• A Eukaryotic Molecular Target Candidate of Roxithromycin: Fungal Differentiation as a Sensitive Drug Target Analysis System

  2013cited by this paper
• Identification and Characterization of the Direct Interaction between Methotrexate (MTX) and High-Mobility Group Box 1 (HMGB1) Protein

  2013cited by this paper
• Target identification for small bioactive molecules: finding the needle in the haystack.

  2013cited by this paper
• Short peptides as biosensor transducers

  2012cited by this paper
• Intrinsically disordered proteins and novel strategies for drug discovery

  2012cited by this paper
• The antitumor agent doxorubicin binds to Fanconi anemia group F protein.

  2012cited by this paper
• Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen.

  2012cited by this paper
• Target discovery from data mining approaches.

  2012cited by this paper
• GuiTope: an application for mapping random-sequence peptides to protein sequences

  2012cited by this paper
• Novel flavonoids with antiproliferative activities against breast cancer cells.

  2011cited by this paper
• The identification of cellular targets of 17β estradiol using a lytic (T7) cDNA phage display approach.

  2011cited by this paper
• Peptide Phage Display as a Tool for Drug Discovery: Targeting Membrane Receptors

  2011cited by this paper
• Bioinformatics Resources and Tools for Phage Display

  2011cited by this paper
• A Screening of a Library of T7 Phage-Displayed Peptide Identifies E2F-4 as an Etoposide-Binding Protein

  2011cited by this paper
• Cyclosporin A Associated Helicase-Like Protein Facilitates the Association of Hepatitis C Virus RNA Polymerase with Its Cellular Cyclophilin B

  2011cited by this paper
• Screening of a library of T7 phage-displayed peptides identifies alphaC helix in 14-3-3 protein as a CBP501-binding site.

  2011cited by this paper
• Camptothecin (CPT) directly binds to human heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) and inhibits the hnRNP A1/topoisomerase I interaction.

  2011cited by this paper
• Binding region and interaction properties of sulfoquinovosylacylglycerol (SQAG) with human vascular endothelial growth factor 165 revealed by biosensor-based assays

  2011cited by this paper
• The anti-tumor antibiotic PD 113,271 binds to microtubule-associated protein 1B (MAP1B)

  2011cited by this paper
• Chapter 3:Quantitative Analysis of Peptide Libraries

  2011cited by this paper
• Use of phage display technology for the determination of the targets for small-molecule therapeutics

  2010cited by this paper
• Flecainide prevents catecholaminergic polymorphic ventricular tachycardia in mice and humans

  2009cited by this paper
• Predicting new molecular targets for known drugs

  2009cited by this paper
• Phage display: a useful tool for malaria research?

  2008cited by this paper
• A photoimmobilisation strategy that maximises exploration of chemical space in small molecule affinity selection and target discovery.

  2007cited by this paper
• A SNARE–adaptor interaction is a new mode of cargo recognition in clathrin-coated vesicles

  2007cited by this paper
• Molecular pharmacology and pharmacogenomics of artemisinin and its derivatives in cancer cells.

  2006cited by this paper
• RELIC – A bioinformatics server for combinatorial peptide analysis and identification of protein‐ligand interaction sites

  2004cited by this paper
• Cyclosporin A suppresses replication of hepatitis C virus genome in cultured hepatocytes

  2003cited by this paper
• Flavonoids: a review of probable mechanisms of action and potential applications.

  2001cited by this paper
• The solution structure of the complex of Lactobacillus casei dihydrofolate reductase with methotrexate.

  1998cited by this paper
• Angiostatin: a novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma.

  1994cited by this paper
• Calcineurin phosphatase activity in T lymphocytes is inhibited by FK 506 and cyclosporin A.

  1992cited by this paper
• Preliminary report: effect of encainide and flecainide on mortality in a randomized trial of arrhythmia suppression after myocardial infarction.

  1989cited by this paper
• Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface.

  1985cited by this paper
• The Biosynthesis of the Pyrenocines in Cultures of Pyrenochaeta terrestris.

  1985cited by this paper
• Macrolide antibiotics. Chemistry, biology, and practice

  1985cited by this paper
• Novel antitumor agents CI-920, PD 113,270 and PD 113,271. II. Isolation and characterization.

  1983cited by this paper
• A SULFOLIPID IN PLANTS.

  1959cited by this paper

• Identification of novel human IgE-binding peptides from a phage display library for total IgE detection

  2025cites this paper
• Rationally designed self-assembled peptide nanofibers provoke robust humoral immunity against nervous necrosis virus

  2025cites this paper
• Targeted Glioma Therapy via TMVP1 Peptide-Modified FLT4 Liposomes: A Novel Molecular Probe Strategy

  2025cites this paper
• Development of Uniform Ribosome Display Technology Enabling Easy and Efficient Identification of Full‐Length Proteins that Interact with Bioactive Small and Large Molecules

  2024cites this paper
• Integrative Network Pharmacology Unveils Limonia acidissima as a Potential Natural Product for Targeting Cancer

  2024cites this paper
• Single-chain variable fragment-based immunoassay for bioactive natural products

  2023cites this paper
• Targeting of Tetraspanin CD81 with Monoclonal Antibodies and Small Molecules to Combat Cancers and Viral Diseases

  2023cites this paper
• Targeted Killing of Staphylococcus aureus Using Specific Peptides Displayed on Yeast Vacuoles

  2023cites this paper
• Streptavidin-Coated Solid-Phase Extraction (SPE) Tips for Antibody Phage Display Biopanning.

  2023cites this paper
• Editorial: Phage display: Technique and applications

  2022cites this paper
• Exploring and exploiting plant cyclic peptides for drug discovery and development

  2021cites this paper
• Application of Artificial Intelligence in Medicine: An Overview

  2021cites this paper
• Potential of Phage Display Antibody Technology for Cardiovascular Disease Immunotherapy

  2021cites this paper
• Screening of specific binding peptides using phage-display techniques and their biosensing applications

  2021cites this paper