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Quantitation of oxylipins in fish and algae oil supplements using optimized hydrolysis procedures and ultra-high performance liquid chromatography coupled to tandem mass-spectrometry.

S. Emami,Zhichao Zhang,A. Taha

Published 2020 in Journal of Agricultural and Food Chemistry

ABSTRACT

Fish and algae oil supplements are enriched with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are precursors to oxidized fatty acids, known as oxylipins. Here, we optimized a base hydrolysis method for measuring oxylipins in oil with UPLC-MS/MS, and quantified them in fish and algae oil supplements. Hydrolysis of 2 µL of oil with sodium carbonate resulted in greater oxylipin concentrations and minimal matrix effects, compared to higher oil volumes (10, 20 and 30 µL). Oxylipin yield was higher when oil was hydrolyzed in methanol containing 0.1% acetic acid and 0.1% butylated-hydroxytoluene, compared to no solvent, and using sodium hydroxide versus sodium carbonate. Oxylipins extracted from 2 µL oil using sodium hydroxide in solvent showed that EPA-derived oxylipins were most abundant in fish oil (84-87%), whereas DHA-oxylipins were abundant in algae oil (83%). This study shows that fish and algae oils are direct sources of EPA- and DHA-derived oxylipins.

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