Curcumin protects bone biomechanical properties and microarchitecture in type 2 diabetic rats with osteoporosis via the TGFβ/Smad2/3 pathway

Type 2 diabetic osteoporosis (T2DOP) has become a common secondary cause of osteoporosis that accelerates bone loss and leads to bone fractures. The aim of the current study was to investigate the association between the anti-osteoporotic effect of curcumin (Cur) and the transforming growth factor (TGF)β/Smads signaling pathway. Male Sprague-Dawley rats were used in the experiments. The type 2 diabetes mellitus (T2DM) animals were treated with Cur for 8 weeks and blood lipid markers, bone microstructure and bone biomechanics were then evaluated. The mRNA expression levels of TGFβ1, type I TGFβ receptor (TβRI), TβRII and Smad2/3 were determined using reverse transcription-quantitative PCR (RT-qPCR) and immunohistochemistry. The body weight of rats with type 2 diabetes-induced osteoporosis increased (P<0.05), while the lipid (total cholesterol, triglyceride and low-density lipoprotein) and fasting blood glucose levels were decreased by Cur (P<0.05). In addition, Cur significantly improved bone biomechanical properties (maximum load, breaking load, elastic load and the bone rigidity coefficient) and preserved bone microarchitecture (P<0.05). The RT-qPCR and IHC results revealed that Cur increased TGFβ1, TβRI, TβRII and Smad2/3 expression levels and promoted Smad2/3 phosphorylation in bones. The present results also indicated that Cur regulated lipid and glucose levels, improved bone biomechanical properties and preserved bone microarchitecture, and that these effects may be mediated via TGFβ/Smad2/3 pathway activation.

• Publication year

  2020

• Venue

  Experimental and Therapeutic Medicine

• Publication date

  2020-06-25

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.3892/etm.2020.8943PMID 32765696PMCID 7401480
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Sargassum integerrimum inhibits oestrogen deficiency and hyperlipidaemia-induced bone loss by upregulating nuclear factor (erythroid-derived 2)-like 2 in female rats

  2019cited by this paper
• New insights into the mechanisms of diabetic complications: role of lipids and lipid metabolism

  2019cited by this paper
• Curcumin improves the effect of a reduced insulin dose on glycemic control and oxidative stress in streptozotocin‐diabetic rats

  2019cited by this paper
• Effect of Zishen Jiangtang Pill, a Chinese Herbal Product, on Rats with Diabetic Osteoporosis

  2018cited by this paper
• Epidemiology and correlates of osteoporotic fractures among type 2 diabetic patients

  2018cited by this paper
• Pulsed electromagnetic fields (PEMF) attenuate changes in vertebral bone mass, architecture and strength in ovariectomized mice.

  2018cited by this paper
• Amelioration of streptozotocin-induced type 2 diabetes mellitus in Wistar rats by arachidonic acid.

  2018cited by this paper
• Curcumin, a Compound from Natural Sources, a True Scientific Challenge – A Review

  2017cited by this paper
• The protective effects of silibinin in the treatment of streptozotocin-induced diabetic osteoporosis in rats.

  2017cited by this paper
• Research on the correlation of diabetes mellitus complicated with osteoporosis with lipid metabolism, adipokines and inflammatory factors and its regression analysis.

  2017cited by this paper
• Use of drugs for osteoporosis treatment in patients with type 2 diabetes mellitus : population-based cohort study

  2017influential reference
• Curcumin and insulin resistance—Molecular targets and clinical evidences

  2016cited by this paper
• High-fat diet combined with low-dose streptozotocin injections induces metabolic syndrome in Macaca mulatta

  2015cited by this paper
• Curcumin improves bone microarchitecture in glucocorticoid-induced secondary osteoporosis mice through the activation of microRNA-365 via regulating MMP-9.

  2015cited by this paper
• Insulin, osteoblasts, and energy metabolism: why bone counts calories.

  2014cited by this paper
• Suppression of the TGF-β/Smad signaling pathway and inhibition of hepatic stellate cell proliferation play a role in the hepatoprotective effects of curcumin against alcohol-induced hepatic fibrosis.

  2014cited by this paper
• Effects of Fish Oil on Cytokines, Glycemic Control, Blood Pressure, and Serum Lipids in Patients with Type 2 Diabetes Mellitus

  2013influential reference
• [The relationship between bone and glucose/lipid metabolism].

  2013cited by this paper
• Curcumin Protects against Ovariectomy-Induced Bone Changes in Rat Model

  2012cited by this paper
• Insulin resistance in type 2 diabetes mellitus may be related to bone mineral density.

  2012cited by this paper
• Methionine-Restricted C57BL/6J Mice Are Resistant to Diet-Induced Obesity and Insulin Resistance but Have Low Bone Density

  2012cited by this paper
• Type 2 diabetes and bone

  2012cited by this paper
• Bisphosphonate-related osteonecrosis of the jaws. A severe side effect of bisphosphonate therapy.

  2012cited by this paper
• Curcumin improves bone microarchitecture and enhances mineral density in APP/PS1 transgenic mice.

  2011cited by this paper
• Regulation effects on abnormal glucose and lipid metabolism of TZQ-F, a new kind of Traditional Chinese Medicine.

  2010cited by this paper
• Effects of curcumin on the skeletal system in rats

  2010cited by this paper
• A study of the comparative effects of hawthorn fruit compound and simvastatin on lowering blood lipid levels.

  2009cited by this paper
• Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases.

  2009cited by this paper
• TGF-β1-induced Migration of Bone Mesenchymal Stem Cells Couples Bone Resorption and Formation

  2009cited by this paper
• Curcumin suppresses increased bone resorption by inhibiting osteoclastogenesis in rats with streptozotocin-induced diabetes.

  2009cited by this paper
• Chemical approaches toward preparation of water-soluble curcumin derivatives

  2009cited by this paper
• The effects of shockwave on bone healing and systemic concentrations of nitric oxide (NO), TGF-beta1, VEGF and BMP-2 in long bone non-unions.

  2009cited by this paper
• Beneficial effects of curcumin on hyperlipidemia and insulin resistance in high-fat-fed hamsters.

  2008cited by this paper
• What the Back of the Object Looks Like: 3D Reconstruction from Line Drawings without Hidden Lines

  2008cited by this paper
• The impact of osteoporosis medication beliefs and side-effect experiences on non-adherence to oral bisphosphonates*

  2007cited by this paper
• TGF-beta1 as a marker of delayed fracture healing.

  2006cited by this paper
• Curcumin: the story so far.

  2005cited by this paper
• TGF-beta1 as a marker of delayed fracture healing.

  2005cited by this paper
• Activation of the AMP-activated Protein Kinase by the Anti-diabetic Drug Metformin in Vivo

  2004cited by this paper
• Evaluation of the Image-Pro Plus 4.5 software for automatic counting of labeled nuclei by PCNA immunohistochemistry.

  2004cited by this paper
• Long-term prevention of renal insufficiency, excess matrix gene expression, and glomerular mesangial matrix expansion by treatment with monoclonal antitransforming growth factor-beta antibody in db/db diabetic mice.

  2000cited by this paper
• Inhibition of TGF-beta receptor signaling in osteoblasts leads to decreased bone remodeling and increased trabecular bone mass.

  1999cited by this paper
• The use of sodium fluoride, vitamin D and calcium supplements in the treatment of patients with axial osteoporosis

  1976cited by this paper
• Antacid Properties of Calcium, Magnesium, and Aluminum Salts of Water-Insoluble Aliphatic Acids

  1963cited by this paper

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  2026cites this paper
• [Curcumin improves osteoporosis in type 2 diabetic mice by inhibiting RANBP3L expression].

  2026cites this paper
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  2025cites this paper
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  2025cites this paper
• Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus

  2025cites this paper
• Emerging Roles of Natural Compounds in Osteoporosis: Regulation, Molecular Mechanisms and Bone Regeneration

  2024cites this paper
• Association between triglycerides and lumbar bone mineral density in Chinese patients with osteoporotic fractures: a retrospective cross-sectional study

  2024cites this paper
• Regulation of Curcumin on Follicle Initiation Rate in Diminished Ovarian Reserve.

  2024cites this paper
• Role of oxidative stress in impaired type II diabetic bone repair: scope for antioxidant therapy intervention?

  2024cites this paper
• Soy Peptide Ameliorate TGF-β1-Mediated Osteoblast Differentiation through Smad and MAPK Signaling Pathways.

  2024cites this paper
• Nutraceuticals and Functional Foods: A Comprehensive Review of Their Role in Bone Health

  2024cites this paper
• Effect of Liraglutide on Osteoporosis in a Rat Model of Type 2 Diabetes Mellitus: A Histological, Immunohistochemical, and Biochemical Study.

  2023cites this paper
• Regulation of adipogenesis by exosomal milk miRNA

  2023cites this paper
• Food Polyphenols and Type II Diabetes Mellitus: Pharmacology and Mechanisms

  2023cites this paper
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  2023cites this paper
• Curcumin nanoparticles and the therapeutic potential of curcumin for musculoskeletal disorders.

  2023cites this paper
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  2023cites this paper
• Dietary polyphenols and osteoporosis: molecular mechanisms involved

  2022cites this paper
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  2022cites this paper
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  2022cites this paper
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  2022cites this paper
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  2022cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper