Sound Vibration-Triggered Epigenetic Modulation Induces Plant Root Immunity Against Ralstonia solanacearum

Sound vibration (SV) is one of the several environmental stimuli that induce physiological changes in plants including changes in plant immunity. Immune activation is a complicated process involving epigenetic modifications, however, SV-induced epigenetic modifications remain unexplored. Here, we performed an integrative analysis comprising chromatin immunoprecipitation (ChIP) and microRNA sequencing (miRNA-seq) to understand the role of SV-mediated epigenetic modifications in immune activation in Arabidopsis thaliana against the root pathogen Ralstonia solanacearum. Plants exposed to SV (10 kHz) showed abundant H3K27me3 modification in the promoter regions of aliphatic glucosinolate biosynthesis and cytokinin signaling genes, leading to transcriptional changes that promote immunity. Additionally, 10 kHz SV down-regulated miR397b expression, thus activating three target LACCASE transcripts that mediate cell wall reinforcement via lignin accumulation. Taken together, SV triggers epigenetic modification of genes involved in secondary metabolite biosynthesis, defense hormone signaling, and pre-formed defense in A. thaliana, leading to the activation of plant immunity against R. solanacearum.

• Publication year

  2020

• Venue

  Frontiers in Microbiology

• Publication date

  2020-08-21

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3389/fmicb.2020.01978PMID 32973716PMCID 7472266
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Sequence Analysis

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• Evolution of Disease Defense Genes and Their Regulators in Plants

  2019cited by this paper
• History and Status of Selected Hosts of the Ralstonia solanacearum Species Complex Causing Bacterial Wilt in Brazil

  2018cited by this paper
• Plant Immunity: From Signaling to Epigenetic Control of Defense.

  2018cited by this paper
• Transmembrane transport and stress response genes play an important role in adaptation of Arabidopsis halleri to metalliferous soils

  2018cited by this paper
• Beyond Chemical Triggers: Evidence for Sound-Evoked Physiological Reactions in Plants

  2018cited by this paper
• Lignins: Biosynthesis and Biological Functions in Plants

  2018cited by this paper
• Modify the Histone to Win the Battle: Chromatin Dynamics in Plant–Pathogen Interactions

  2018cited by this paper
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  2018cited by this paper
• Epigenomics of Plant Responses to Environmental Stress

  2018cited by this paper
• Analysis of RDR1/RDR2/RDR6‐independent small RNAs in Arabidopsis thaliana improves MIRNA annotations and reveals unexplained types of short interfering RNA loci

  2018cited by this paper
• Plant MicroRNAs in Cross-Kingdom Regulation of Gene Expression

  2018cited by this paper
• SG2-Type R2R3-MYB Transcription Factor MYB15 Controls Defense-Induced Lignification and Basal Immunity in Arabidopsis

  2017cited by this paper
• Suppression of cotton leaf curl disease symptoms in Gossypium hirsutum through over expression of host-encoded miRNAs.

  2017cited by this paper
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  2017cited by this paper
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  2017cited by this paper
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  2017cited by this paper
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  2017cited by this paper
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  2017cited by this paper
• Positive regulatory role of sound vibration treatment in Arabidopsis thaliana against Botrytis cinerea infection

  2017cited by this paper
• Interspecific Potato Breeding Lines Display Differential Colonization Patterns and Induced Defense Responses after Ralstonia solanacearum Infection

  2017cited by this paper
• How Auxin and Cytokinin Phytohormones Modulate Root Microbe Interactions

  2016cited by this paper
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  2016cited by this paper
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  2016cited by this paper
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  2016cited by this paper
• MicroRNA in Control of Gene Expression: An Overview of Nuclear Functions

  2016cited by this paper
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  2016cited by this paper
• Nitric Oxide Modulates Histone Acetylation at Stress Genes by Inhibition of Histone Deacetylases1[OPEN]

  2016cited by this paper
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  2016cited by this paper
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  2016cited by this paper
• Heterologous expression of chloroplast‐localized geranylgeranyl pyrophosphate synthase confers fast plant growth, early flowering and increased seed yield

  2015cited by this paper
• Lignin Extraction and Quantification, a Tool to Monitor Defense Reaction at the Plant Cell Wall Level

  2015cited by this paper
• The symbiotic transcription factor MtEFD and cytokinins are positively acting in the Medicago truncatula and Ralstonia solanacearum pathogenic interaction.

  2014cited by this paper
• The Arabidopsis miR472-RDR6 Silencing Pathway Modulates PAMP- and Effector-Triggered Immunity through the Post-transcriptional Control of Disease Resistance Genes

  2014cited by this paper
• Advances in Effects of Sound Waves on Plants

  2014influential reference
• PROTOCOL: Chromatin Immunoprecipitation from Arabidopsis Tissues

  2014cited by this paper
• HTSeq—a Python framework to work with high-throughput sequencing data

  2014cited by this paper
• Cytokinins

  2014cited by this paper
• A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis.

  2014cited by this paper
• In Vitro Activity of Glucosinolates and Their Degradation Products against Brassica-Pathogenic Bacteria and Fungi

  2014cited by this paper
• Plants respond to leaf vibrations caused by insect herbivore chewing

  2014cited by this paper
• TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions

  2013cited by this paper
• Recent Advances in Plant NLR Structure, Function, Localization, and Signaling

  2013cited by this paper
• LACCASE Is Necessary and Nonredundant with PEROXIDASE for Lignin Polymerization during Vascular Development in Arabidopsis[C][W]

  2013cited by this paper
• STAR: ultrafast universal RNA-seq aligner

  2013cited by this paper
• Functional roles of histone modification, chromatin remodeling and microRNAs in Arabidopsis flower development.

  2013cited by this paper
• Arabidopsis wat1 (walls are thin1)-mediated resistance to the bacterial vascular pathogen, Ralstonia solanacearum, is accompanied by cross-regulation of salicylic acid and tryptophan metabolism.

  2013cited by this paper
• Transcriptome Analysis of Quantitative Resistance-Specific Response upon Ralstonia solanacearum Infection in Tomato

  2012cited by this paper
• Functional and Transcriptome Analysis Reveals an Acclimatization Strategy for Abiotic Stress Tolerance Mediated by Arabidopsis NF-YA Family Members

  2012cited by this paper
• Cytokinin Regulates the Activity of Reproductive Meristems, Flower Organ Size, Ovule Formation, and Thus Seed Yield in Arabidopsis thaliana[C][W][OA]

  2011cited by this paper
• MicroRNAs as master regulators of the plant NB-LRR defense gene family via the production of phased, trans-acting siRNAs.

  2011cited by this paper
• Non-coding RNAs as regulators of gene expression and epigenetics.

  2011cited by this paper
• Agrobacterium-produced and exogenous cytokinin-modulated Agrobacterium-mediated plant transformation.

  2010cited by this paper
• edgeR: a Bioconductor package for differential expression analysis of digital gene expression data

  2009cited by this paper
• Application of acoustic frequency technology to protected vegetable production

  2009influential reference
• ChIP–seq: advantages and challenges of a maturing technology

  2009cited by this paper
• Model-based Analysis of ChIP-Seq (MACS)

  2008influential reference
• Nuclear gene silencing directs reception of long-distance mRNA silencing in Arabidopsis

  2007cited by this paper
• Construction of small RNA cDNA libraries for deep sequencing.

  2007cited by this paper
• Chromatin modifications and their function.

  2007cited by this paper
• Erwinia carotovora elicitors and Botrytis cinerea activate defense responses in Physcomitrella patens

  2007cited by this paper
• Post-transcriptional small RNA pathways in plants: mechanisms and regulations.

  2006cited by this paper
• Transcriptome analysis of Arabidopsis clubroots indicate a key role for cytokinins in disease development.

  2006cited by this paper
• The plant immune system

  2006cited by this paper
• Systemic Effects of Heterobasidion annosum on Ferulic Acid Glucoside and Lignin of Presymptomatic Ponderosa Pine Phloem, and Potential Effects on Bark-Beetle-Associated Fungi

  2003cited by this paper
• Chromatin Modifications and Their Function

  year unknowncited by this paper

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• Information and Communication Theoretical Foundations of the Internet of Plants, Principles, Challenges, and Future Directions

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• Plants detect and respond to sounds

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