Molecular characterization of Urosporidium tapetis sp. nov., a haplosporidian hyperparasite infecting metacercaria of Parvatrema duboisi (Dollfus 1923), a trematode parasite of Manila clam Ruditapes philippinarum on the west coast of Korea.

Young-Ghan Cho,Hyun-Sil Kang,Cuong Thanh Le,Mun Gyeong Kwon,Min-Seok Jang,Kwang-Sik Choi

Published 2020 in Journal of Invertebrate Pathology

ABSTRACT

Recently, a putative new hyperparasitic haplosporidian in the genus Urosporidium was identified from metacercaria of the trematode Parvatrema duboisi infecting Manila clam Ruditapes philippinarum on the west coast of Korea. In this study, we applied small subunit ribosomal DNA (SSU rDNA) sequences as a marker to substantiate the phylogenetic relationship of the unidentified Urosporidium within the Order Haplosporida. In our phylogenetic analysis, the 1,890 bp of SSU rDNA sequences obtained were closely related to a haplosporidian parasite forming a sister clade to Urosporidium group, although the gene sequences were only 89.22-89.70% similar to Urosporidium spp. Such molecular phylogenetic distance within the genus suggested that the unidentified Urosporidium is a new member of the genus. Accordingly, we report the unidentified haplosporidian hyperparasite as Urosporidium tapetis sp. nov.

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