Hyperpolarization moves S4 sensors inward to open MVP, a methanococcal voltage-gated potassium channel

MVP, a Methanococcus jannaschii voltage-gated potassium channel, was cloned and shown to operate in eukaryotic and prokaryotic cells. Like pacemaker channels, MVP opens on hyperpolarization using S4 voltage sensors like those in classical channels activated by depolarization. The MVP S4 span resembles classical sensors in sequence, charge, topology and movement, traveling inward on hyperpolarization and outward on depolarization (via canaliculi in the protein that bring the extracellular and internal solutions into proximity across a short barrier). Thus, MVP opens with sensors inward indicating a reversal of S4 position and pore state compared to classical channels. Homolo-gous channels in mammals and plants are expected to function similarly.

• Publication year

  2003

• Venue

  Nature Neuroscience

• Publication date

  2003-04-01

• Fields of study

  Biology, Medicine, Physics, Environmental Science

• Identifiers
  DOI 10.1038/nn1028PMID 12640457
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Voltage-sensing mechanism is conserved among ion channels gated by opposite voltages

  2002cited by this paper
• Crystal structure and mechanism of a calcium-gated potassium channel

  2002cited by this paper
• Voltage-Controlled Gating at the Intracellular Entrance to a Hyperpolarization-Activated Cation Channel

  2002cited by this paper
• Blocker State Dependence and Trapping in Hyperpolarization-Activated Cation Channels

  2001cited by this paper
• Properties of Hyperpolarization-Activated Pacemaker Current Defined by Coassembly of Hcn1 and Hcn2 Subunits and Basal Modulation by Cyclic Nucleotide

  2001cited by this paper
• Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors

  2001cited by this paper
• Histidine Scanning Mutagenesis of Basic Residues of the S4 Segment of the Shaker K+ Channel

  2001cited by this paper
• A Prokaryotic Voltage-Gated Sodium Channel

  2001cited by this paper
• Immunity to K1 killer toxin: internal TOK1 blockade.

  2001cited by this paper
• Conformational changes in S6 coupled to the opening of cyclic nucleotide-gated channels.

  2001cited by this paper
• KCNKØ: opening and closing the 2-P-domain potassium leak channel entails "C-type" gating of the outer pore.

  2001cited by this paper
• Molecular diversity of pacemaker ion channels.

  2001cited by this paper
• Functional Roles of Charged Residues in the Putative Voltage Sensor of the HCN2 Pacemaker Channel*

  2000cited by this paper
• The voltage sensor in voltage-dependent ion channels.

  2000cited by this paper
• Change of pore helix conformational state upon opening of cyclic nucleotide-gated channels.

  2000cited by this paper
• Blocker protection in the pore of a voltage-gated K+ channel and its structural implications

  2000cited by this paper
• Molecular characterization of a slowly gating human hyperpolarization-activated channel predominantly expressed in thalamus, heart, and testis.

  1999cited by this paper
• Two pacemaker channels from human heart with profoundly different activation kinetics

  1999cited by this paper
• Ion channels in microbes.

  1999cited by this paper
• Distinct Molecular Bases for pH Sensitivity of the Guard Cell K+ Channels KST1 and KAT1*

  1999cited by this paper
• Contribution of the selectivity filter to inactivation in potassium channels.

  1999cited by this paper
• A molecular target for viral killer toxin: TOK1 potassium channels.

  1999cited by this paper
• A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.

  1999cited by this paper
• Functional characterization of a potassium-selective prokaryotic glutamate receptor

  1999cited by this paper
• The moving parts of voltage-gated ion channels

  1998cited by this paper
• Modulation of C-type inactivation by K+ at the potassium channel selectivity filter.

  1998cited by this paper
• Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain.

  1998cited by this paper
• Protein Rearrangements Underlying Slow Inactivation of the Shaker K+ Channel

  1998cited by this paper
• Voltage-dependent Gating of Single Wild-Type and S4 Mutant KAT1 Inward Rectifier Potassium Channels

  1998cited by this paper
• Voltage-dependent proton transport by the voltage sensor of the Shaker K+ channel.

  1997cited by this paper
• Selectivity Changes during Activation of Mutant Shaker Potassium Channels

  1997cited by this paper
• Molecular basis of charge movement in voltage-gated sodium channels.

  1996cited by this paper
• Complete Genome Sequence of the Methanogenic Archaeon, Methanococcus jannaschii

  1996cited by this paper
• Fast inactivation causes rectification of the IKr channel

  1996cited by this paper
• Voltage-sensing residues in the S2 and S4 segments of the Shaker K+ channel.

  1996cited by this paper
• ORK1, a potassium-selective leak channel with two pore domains cloned from Drosophila melanogaster by expression in Saccharomyces cerevisiae.

  1996cited by this paper
• The inward rectification mechanism of the HERG cardiac potassium channel

  1996cited by this paper
• TrkH and its homolog, TrkG, determine the specificity and kinetics of cation transport by the Trk system of Escherichia coli

  1995cited by this paper
• A new family of outwardly rectifying potassium channel proteins with two pore domains in tandem

  1995cited by this paper
• Evidence for voltage-dependent S4 movement in sodium channels.

  1995cited by this paper
• Regulation of voltage dependence of the KAT1 channel by intracellular factors

  1995cited by this paper
• Voltage gating of ion channels

  1994cited by this paper
• Shaker potassium channel gating. I: Transitions near the open state

  1994cited by this paper
• Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.

  1992cited by this paper
• TRK1 and TRK2 encode structurally related K+ transporters in Saccharomyces cerevisiae

  1991cited by this paper
• A quantitative description of membrane current and its application to conduction and excitation in nerve. 1952.

  1990cited by this paper
• Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.

  1990cited by this paper
• A mechanosensitive ion channel in the yeast plasma membrane.

  1988cited by this paper
• Characterization of single pacemaker channels in cardiac sino-atrial node cells

  1986cited by this paper
• A quantitative description of membrane current and its application to conduction and excitation in nerve

  1952cited by this paper

• Structure mirroring function: What's the 'matter' with the funny current?

  2025cites this paper
• Exploring Flexibility and Folding Patterns Throughout Time in Voltage Sensors

  2023cites this paper
• Electromechanical Coupling in the Hyperpolarization-Activated K+ Channel KAT1

  2020cites this paper
• Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression.

  2016cites this paper
• Functional Site-Directed Fluorometry.

  2015cites this paper
• Structure of potassium channels

  2015cites this paper
• Outward Rectification of Voltage-Gated K+ Channels Evolved at Least Twice in Life History

  2015cites this paper
• Biochemical and Structural Analysis of the Hyperpolarization-Activated K+ Channel MVP

  2014cites this paper
• cAMP Control of HCN2 Channel Mg2+ Block Reveals Loose Coupling between the Cyclic Nucleotide-Gating Ring and the Pore

  2014cites this paper
• Using yeast to study potassium channel function and interactions with small molecules.

  2013cites this paper
• Identification of Putative Potassium Channel Homologues in Pathogenic Protozoa

  2012cites this paper
• Identification and Analysis of Cation Channel Homologues in Human Pathogenic Fungi

  2012cites this paper
• Charge movement in gating-locked HCN channels reveals weak coupling of voltage sensors and gate

  2012cites this paper
• Development of a tethered biomembrane biosensing platformfor the incorporation of ion channels

  2011cites this paper
• Inverse problems in ion channel modelling

  2011cites this paper
• Avian magnetite-based magnetoreception: a physiologist's perspective

  2010cites this paper
• Probing S4 and S5 segment proximity in mammalian hyperpolarization-activated HCN channels by disulfide bridging and Cd2+ coordination

  2009cites this paper
• Two Separate Interfaces between the Voltage Sensor and Pore Are Required for the Function of Voltage-Dependent K+ Channels

  2009cites this paper
• Niflumic Acid Alters Gating of HCN2 Pacemaker Channels by Interaction with the Outer Region of S4 Voltage Sensing Domains

  2009cites this paper
• Mathematical Modelling and Simulation of Ion Channels

  2009cites this paper
• Ion channels in microbes.

  2008cites this paper
• Cellular electrophysiology of cardiac pacemaker channel-implications on novel drug and gene therapies development

  2008cites this paper
• Genetic selection of activatory mutations in KcsA

  2008cites this paper
• Intracellular Mg2+ is a voltage-dependent pore blocker of HCN channels.

  2008cites this paper
• Gating mechanisms in hyperpolarization-activated cyclic nucleotide-gated ion channels

  2007cites this paper
• Sensing, Signal Transduction, and Posttranslational Modification

  2007cites this paper
• Kinetic Relationship between the Voltage Sensor and the Activation Gate in spHCN Channels

  2007cites this paper
• Patch Clamp and Phenotypic Analyses of a Prokaryotic Cyclic Nucleotide-gated K+ Channel Using Escherichia coli as a Host*

  2007cites this paper
• Interactive domains between pore loops of the yeast K+ channel TOK1 associate with extracellular K+ sensitivity.

  2006cites this paper
• Molecular Template for a Voltage Sensor in a Novel K+ Channel. II. Conservation of a Eukaryotic Sensor Fold in a Prokaryotic K+ Channel

  2006cites this paper
• Charakterisierung eines zyklisch Nukleotid-gesteuerten Ionenkanals aus Mesorhizobium loti

  2006cites this paper
• Molecular Template for a Voltage Sensor in a Novel K+ Channel. I. Identification and Functional Characterization of KvLm, a Voltage-gated K+ Channel from Listeria monocytogenes

  2006influential citation
• Crystal Structure of a Mammalian Voltage-Dependent Shaker Family K+ Channel

  2005cites this paper
• Prokaryotic K(+) channels: from crystal structures to diversity.

  2005influential citation
• The S4 voltage sensor packs against the pore domain in the KAT1 voltage-gated potassium channel.

  2005cites this paper
• Electrostatic interaction of a K+ channel RCK domain with charged membrane surfaces.

  2005cites this paper
• The Role of Bacterial Channels in Cell Physiology

  2005cites this paper
• Voltage-Gated K+ Channels

  2005cites this paper
• Architecture of a membrane protein: The voltage-gated K + channel

  2004cites this paper
• How S4 Segments Move Charge. Let Me Count the Ways

  2004cites this paper
• Changes in Local S4 Environment Provide a Voltage-sensing Mechanism for Mammalian Hyperpolarization–activated HCN Channels

  2004influential citation
• S4 Movement in a Mammalian HCN Channel

  2004cites this paper
• Orientation of Arabidopsis thaliana KAT1 Channel in the Plasma Membrane

  2004cites this paper
• Arranging the elements of the potassium channel: the T1 domain occludes the cytoplasmic face of the channel

  2004cites this paper
• Molecular Coupling between Voltage Sensor and Pore Opening in the Arabidopsis Inward Rectifier K+ Channel KAT1

  2003cites this paper
• Charybdotoxin binding in the I(Ks) pore demonstrates two MinK subunits in each channel complex.

  2003cites this paper
• Gain‐of‐function mutations indicate that Escherichia coli Kch forms a functional K+ conduit in vivo

  2003cites this paper
• MjK1, a K+ channel from M. jannaschii, mediates K+ uptake and K+ sensitivity in E. coli

  2003cites this paper
• Commentary How S 4 Segments Move Charge . Let Me Count the Ways

  2003cites this paper