Dynamic and Differential Expression of Duplicated Cxcr4/Cxcl12 Genes Facilitates Antiviral Response in Hexaploid Gibel Carp

Chemokine receptor cxcr4 and its ligand cxcl12 have evolved two paralogs in the teleost lineage. In this study, we have identified four duplicated cxcr4 and cxcl12 genes from hexaploid gibel carp, Carassius gibelio, respectively. Cgcxcr4bs and Cgcxcl12as were dynamically and differentially expressed in immune-related tissues, and significantly up-regulated in head kidney and spleen after crucian carp herpesvirus (CaHV) infection. Blocking Cxcr4/Cxcl12 axis by injecting AMD3100 brought more severe bleeding symptom and lower survival rate in CaHV-infected fish. AMD3100 treatment also suppressed the up-regulation of key antiviral genes in head kidney and spleen, and resulted in more acute replication of CaHV in vivo. Consistently, the similar suppression of up-regulated expression of key antiviral genes were also observed in CAB cells treated by AMD3100 after poly(I:C) stimulation. Finally, MAPK3 and JAK/STAT were identified as the possible pathways that CgCxcr4s and CgCxcl12s participate in to promote the antiviral response in vitro.

• Publication year

  2020

• Venue

  Frontiers in Immunology

• Publication date

  2020-09-11

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3389/fimmu.2020.02176PMID 33013914PMCID 7516010
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• CXCR4s in Teleosts: Two Paralogous Chemokine Receptors and Their Roles in Hematopoietic Stem/Progenitor Cell Homeostasis

  2020cited by this paper
• Regain of sex determination system and sexual reproduction ability in a synthetic octoploid male fish

  2020cited by this paper
• Stable Genome Incorporation of Sperm-derived DNA Fragments in Gynogenetic Clone of Gibel Carp

  2020cited by this paper
• The CXC chemokines and CXC chemokine receptors in orange‐spotted grouper (Epinephelus coioides) and their expression after Singapore grouper iridovirus infection

  2019cited by this paper
• Molecular cloning, characterization and expression analysis of MHCI and chemokines CXCR3 and CXCR4 gene from freshwater carp, Catla catla

  2019cited by this paper
• Differential expression of innate and adaptive immune genes in the survivors of three gibel carp gynogenetic clones after herpesvirus challenge

  2019cited by this paper
• The CXCL12/CXCR4 Signaling Axis Retains Neutrophils at Inflammatory Sites in Zebrafish

  2019cited by this paper
• FTRCA1, a Species-Specific Member of finTRIM Family, Negatively Regulates Fish IFN Response through Autophage-Lysosomal Degradation of TBK1

  2019cited by this paper
• The characteristics and pivotal roles of triggering receptor expressed on myeloid cells-1 in autoimmune diseases.

  2019cited by this paper
• The unique structural and functional features of CXCL12

  2018cited by this paper
• Diverse and variable sex determination mechanisms in vertebrates

  2018cited by this paper
• Alternative Splicing Transcripts of Zebrafish LGP2 Gene Differentially Contribute to IFN Antiviral Response

  2018cited by this paper
• Differential interferon system gene expression profiles in susceptible and resistant gynogenetic clones of gibel carp challenged with herpesvirus CaHV

  2018cited by this paper
• Gene retention, fractionation and subgenome differences in polyploid plants

  2018cited by this paper
• Differential expression and functional diversification of diverse immunoglobulin domain‐containing protein (DICP) family in three gynogenetic clones of gibel carp

  2018cited by this paper
• Crucian Carp and Gibel Carp Culture

  2018cited by this paper
• Protective effects of AMD3100 on ischemic stroke by inhibiting inflammation and promoting angiogenesis and neuranagenesis

  2018influential reference
• Detection of Microbial Infections Through Innate Immune Sensing of Nucleic Acids.

  2018cited by this paper
• Divergent Expression Patterns and Function of Two cxcr4 Paralogs in Hermaphroditic Epinephelus coioides

  2018cited by this paper
• Diversity, evolutionary contribution and ecological roles of aquatic viruses

  2018cited by this paper
• Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes

  2017cited by this paper
• The evolutionary significance of polyploidy

  2017cited by this paper
• Natural and artificial polyploids in aquaculture

  2017cited by this paper
• Molecular characterization and expression analysis of the large yellow croaker (Larimichthys crocea) chemokine receptors CXCR2, CXCR3, and CXCR4 after bacterial and poly I:C challenge

  2017cited by this paper
• The SDF-1/CXCR4 axis promotes recovery after spinal cord injury by mediating bone marrow-derived from mesenchymal stem cells

  2017cited by this paper
• The CC and CXC chemokine receptors in channel catfish (Ictalurus punctatus) and their involvement in disease and hypoxia responses.

  2017cited by this paper
• CXCL12a/CXCR4b acts to retain neutrophils in caudal hematopoietic tissue and to antagonize recruitment to an injury site in the zebrafish larva

  2017cited by this paper
• Biological/pathological functions of the CXCL12/CXCR4/CXCR7 axes in the pathogenesis of bladder cancer

  2017cited by this paper
• CXCL12-CXCR4 signaling pathways of orange-spotted, grouper (Epinephelus coioides) contribute to nodavirus infection

  2016cited by this paper
• Complete genome sequence and architecture of crucian carp Carassius auratus herpesvirus (CaHV)

  2016cited by this paper
• The Atlantic salmon genome provides insights into rediploidization

  2016cited by this paper
• The spotted gar genome illuminates vertebrate evolution and facilitates human-to-teleost comparisons

  2016cited by this paper
• Administration of AMD3100 in endotoxemia is associated with pro-inflammatory, pro-oxidative, and pro-apoptotic effects in vivo

  2016cited by this paper
• Targeting CXCR4 by a selective peptide antagonist modulates tumor microenvironment and microglia reactivity in a human glioblastoma model

  2016influential reference
• CXCR4 signaling in health and disease.

  2016cited by this paper
• Cloning and expression of Asian swamp eel (Monopterus albus) cxcr4 paralogues, and their modulation by pathogen infection

  2016cited by this paper
• A CXC chemokine gene, CXCL12, from rock bream, Oplegnathus fasciatus: Molecular characterization and transcriptional profile.

  2015cited by this paper
• Virus genomes and virus-host interactions in aquaculture animals

  2015cited by this paper
• Zebrafish IRF1 Regulates IFN Antiviral Response through Binding to IFNϕ1 and IFNϕ3 Promoters Downstream of MyD88 Signaling

  2015cited by this paper
• Molecular characterization, genomic structure and expressional profiles of a CXC chemokine receptor 4 (CXCR4) from rock bream Oplegnathus fasciatus.

  2015cited by this paper
• Chemokine receptors in Atlantic salmon.

  2015cited by this paper
• Chemokine‐guided cell migration and motility in zebrafish development

  2015influential reference
• Chemokines and other GPCR ligands synergize in receptor-mediated migration of monocyte-derived immature and mature dendritic cells.

  2014cited by this paper
• Evolutionary history of two divergent Dmrt1 genes reveals two rounds of polyploidy origins in gibel carp.

  2014cited by this paper
• p85α recruitment by the CD300f phosphatidylserine receptor mediates apoptotic cell clearance required for autoimmunity suppression

  2014cited by this paper
• Emerging roles for triggering receptor expressed on myeloid cells receptor family signaling in inflammatory diseases

  2014cited by this paper
• Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway.

  2014cited by this paper
• Chemokines and chemokine receptors: positioning cells for host defense and immunity.

  2014cited by this paper
• Innate immune detection of microbial nucleic acids.

  2013cited by this paper
• Neutrophil mobilization via plerixafor-mediated CXCR4 inhibition arises from lung demargination and blockade of neutrophil homing to the bone marrow

  2013cited by this paper
• Stromal cell-derived factor-1 and its receptor CXCR4 in adult neurogenesis after cerebral ischemia

  2013cited by this paper
• Sequencing of the sea lamprey (Petromyzon marinus) genome provides insights into vertebrate evolution

  2013cited by this paper
• The CD300 molecules: an emerging family of regulators of the immune system.

  2013cited by this paper
• Grouper (Epinephelus coioides) CXCR4 is expressed in response to pathogens infection and early stage of development.

  2012cited by this paper
• Possible mechanisms involved in chemokine synergy fine tuning the inflammatory response.

  2012cited by this paper
• Molecular regulation of interferon antiviral response in fish.

  2012cited by this paper
• Comparative Analysis of Syntenic Genes in Grass Genomes Reveals Accelerated Rates of Gene Structure and Coding Sequence Evolution in Polyploid Wheat1[W][OA]

  2012cited by this paper
• A novel nucleo-cytoplasmic hybrid clone formed via androgenesis in polyploid gibel carp

  2011cited by this paper
• Stromal cell‐derived factor‐1/CXCR4 promotes IL‐6 production in human synovial fibroblasts

  2011cited by this paper
• Beneficial Effect of a CXCR4 Agonist in Murine Models of Systemic Inflammation

  2011cited by this paper
• Chemokines: A New Dendritic Cell Signal for T Cell Activation

  2011cited by this paper
• Fish MITA Serves as a Mediator for Distinct Fish IFN Gene Activation Dependent on IRF3 or IRF7

  2011cited by this paper
• Chronic AMD3100 antagonism of SDF-1alpha-CXCR4 exacerbates cardiac dysfunction and remodeling after myocardial infarction.

  2010cited by this paper
• Mechanisms guiding primordial germ cell migration: strategies from different organisms

  2010cited by this paper
• Genetic basis and breeding application of clonal diversity and dual reproduction modes in polyploid Carassius auratus gibelio

  2010cited by this paper
• The bone marrow: a site of neutrophil clearance

  2010cited by this paper
• Fish virus-induced interferon exerts antiviral function through Stat1 pathway.

  2010cited by this paper
• Characterization of Fish IRF3 as an IFN-Inducible Protein Reveals Evolving Regulation of IFN Response in Vertebrates

  2010cited by this paper
• Elucidation of CXCR7-Mediated Signaling Events and Inhibition of CXCR4-Mediated Tumor Cell Transendothelial Migration by CXCR7 Ligands

  2009influential reference
• Distinguishing among evolutionary models for the maintenance of gene duplicates.

  2009cited by this paper
• Fish-Specific Duplicated dmrt2b Contributes to a Divergent Function through Hedgehog Pathway and Maintains Left-Right Asymmetry Establishment Function

  2009cited by this paper
• SDF-1alpha up-regulates interleukin-6 through CXCR4, PI3K/Akt, ERK, and NF-kappaB-dependent pathway in microglia.

  2009cited by this paper
• Chemokine receptor 4 (CXCR4) is part of the lipopolysaccharide “sensing apparatus”

  2008cited by this paper
• Translating an Antagonist of Chemokine Receptor CXCR4: From Bench to Bedside

  2008cited by this paper
• Regulation of CXCR4 signaling.

  2007influential reference
• The medaka draft genome and insights into vertebrate genome evolution

  2007cited by this paper
• Stromal cell-derived factor-1 and CXCR4 receptor interaction in tumor growth and metastasis of breast cancer.

  2006influential reference
• T lymphocytes on the move: chemokines, PI 3-kinase and beyond.

  2006cited by this paper
• From bench to bedside

  2005cited by this paper
• Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype

  2004cited by this paper
• Evolution of signal transduction by gene and genome duplication in fish

  2004cited by this paper
• Safety, Pharmacokinetics, and Antiviral Activity of AMD3100, a Selective CXCR4 Receptor Inhibitor, in HIV-1 Infection

  2004cited by this paper
• Three novel carp CXC chemokines are expressed early in ontogeny and at nonimmune sites.

  2004cited by this paper
• Are all fishes ancient polyploids?

  2004cited by this paper
• Evolution of signal transduction by gene and genome duplication in fish.

  2003cited by this paper
• Molecular cloning and characterization of crucian carp (Carassius auratus L.) interferon regulatory factor 7.

  2003cited by this paper
• Occurrence of polyploidy in the fishes

  2003cited by this paper
• β-Arrestin2 Is Critically Involved in CXCR4-mediated Chemotaxis, and This Is Mediated by Its Enhancement of p38 MAPK Activation*

  2002cited by this paper
• Chemokine receptor homo‐ or heterodimerization activates distinct signaling pathways

  2001cited by this paper
• β-Arrestin Differentially Regulates the Chemokine Receptor CXCR4-mediated Signaling and Receptor Internalization, and This Implicates Multiple Interaction Sites between β-Arrestin and CXCR4*

  2000cited by this paper
• Active movement of T cells away from a chemokine

  2000cited by this paper
• Preservation of duplicate genes by complementary, degenerative mutations.

  1999cited by this paper
• The chemokine SDF‐lα triggers CXCR4 receptor dimerization and activates the JAK/STAT pathway

  1999cited by this paper
• Function of the chemokine receptor CXCR4 in haematopoiesis and in cerebellar development

  1998cited by this paper

• Arachidonic Acid Metabolism Down-Regulation-Mediated Tumor Necrosis Factor Signaling Contributes to Cutaneous Fibrosis and Skull Hyperplasia in Goldfish Hoods

  2025cites this paper
• Expression pattern changes of three homeologs in chemokine activity enhance antiviral response to herpesvirus infection in a newly synthesized alloheptaploid

  2025cites this paper
• Characterization, immune response and antibacterial mechanism of CXCL12 in black rockfish (Sebastes schlegelii).

  2025cites this paper
• EcCXCR4b influences RGNNV proliferation by interacting with the RGNNV capsid protein

  2025influential citation
• Trematode Diplostomum pseudospathaceum inducing differential immune gene expression in sexual and gynogenetic gibel carp (Carassius gibelio): parasites facilitating the coexistence of two reproductive forms of the invasive species

  2024cites this paper
• The Teleost CXCL13-CXCR5 Axis Induces Inflammatory Cytokine Expression through the Akt-NF-κB, p38-AP-1, and p38-NF-κB Pathways.

  2023cites this paper
• Astragalus polysaccharide or β-glucan combined with inactivated vaccine markedly prevent CyHV-2 infection in Carassius auratus gibelio

  2023cites this paper
• Evolutionary traits and functional roles of chemokines and their receptors in the male pregnancy of the Syngnathidae

  2023cites this paper
• CC and CXC chemokines in turbot (Scophthalmus maximus L.): Identification, evolutionary analyses, and expression profiling after Aeromonas salmonicida infection.

  2022cites this paper
• Cooperative antivirus activities of two duplicated viperin homeologs confirmed by CRISPR/Cas9 editing in hexaploid gibel carp

  2022cites this paper
• Two paralogs of CXCR4 in the Japanese sea bass (Lateolabrax japonica) are involved in the immune response of B lymphocytes.

  2022cites this paper
• Functional Divergence of Multiple Duplicated Foxl2 Homeologs and Alleles in a Recurrent Polyploid Fish

  2021cites this paper
• Two Duplicated Ptpn6 Homeologs Cooperatively and Negatively Regulate RLR-Mediated IFN Response in Hexaploid Gibel Carp

  2021cites this paper
• Divergent Antiviral Mechanisms of Two Viperin Homeologs in a Recurrent Polyploid Fish

  2021cites this paper
• CXC chemokines and their receptors in black rockfish (Sebastes schlegelii): Characterization, evolution analyses, and expression pattern after Aeromonas salmonicida infection.

  2021cites this paper
• Diversity of Rainbow Trout Blood B Cells Revealed by Single Cell RNA Sequencing

  2021cites this paper
• The CC and CXC chemokine receptors in turbot (Scophthalmus maximus L.) and their response to Aeromonas salmonicida infection.

  2021cites this paper