CD48 Binds to Heparan Sulfate on the Surface of Epithelial Cells*

CD48 is a member of the immunoglobulin superfamily whose cell surface expression is strikingly up-regulated on the surface of Epstein-Barr virus-infected B cells. To date, no ligand for human CD48 has been characterized. In this study, we show that human recombinant CD48 binds to the glycosaminoglycan heparan sulfate on the surface of human epithelial cells. We have produced a monoclonal antibody (615) against epithelial cell surfaces that blocks this binding and show that it too recognizes heparan sulfate. The specific epitope on heparan sulfate that is recognized by the antibody and is involved in binding is also expressed in vivo on the basolateral surfaces of mucosal epithelium and lamina propria.

• Publication year

  1998

• Venue

  Journal of Biological Chemistry

• Publication date

  1998-09-04

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/jbc.273.36.23367PMID 9722571
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Identification of the Site of Epstein-Barr Virus Persistence In Vivo as a Resting B Cell

  1998cited by this paper
• A ligand for human CD48 on epithelial cells.

  1997cited by this paper
• Identification of the site of Epstein-Barr virus persistence in vivo as a resting B cell

  1997cited by this paper
• Heparan sulfate: a piece of information

  1996cited by this paper
• Lymphocyte Homing and Homeostasis

  1996cited by this paper
• A sensitive assay for detecting low‐affinity interactions at the cell surface reveals no additional ligands for the adhesion pair rat CD2 and CD48

  1995cited by this paper
• Heparan Sulphate and Protein Recognition

  1995cited by this paper
• Characterization of the CD48 gene demonstrates a positive element that is specific to Epstein-Barr virus-immortalized B-cell lines and contains an essential NF-kappa B site

  1995cited by this paper
• Heparin is an adhesive ligand for the leukocyte integrin Mac-1 (CD11b/CD1)

  1995cited by this paper
• A novel form of Epstein-Barr virus latency in normal B cells in vivo.

  1995cited by this paper
• I-type Lectins (*)

  1995cited by this paper
• Identification of a common hyaluronan binding motif in the hyaluronan binding proteins RHAMM, CD44 and link protein.

  1994cited by this paper
• Fine structure of heparan sulfate regulates syndecan-1 function and cell behavior.

  1994cited by this paper
• Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm.

  1994cited by this paper
• Cell surface syndecan-1 on distinct cell types differs in fine structure and ligand binding of its heparan sulfate chains.

  1994cited by this paper
• A soluble multimeric recombinant CD2 protein identifies CD48 as a low affinity ligand for human CD2: divergence of CD2 ligands during the evolution of humans and mice

  1993cited by this paper
• Production and characterization of soluble and transmembrane murine CD2. Demonstration that CD48 is a ligand for CD2 and that CD48 adhesion is regulated by CD2.

  1993cited by this paper
• Specificity studies on the heparin lyases from Flavobacterium heparinum.

  1993cited by this paper
• Platelet/endothelial cell adhesion molecule-1 (CD31)-mediated cellular aggregation involves cell surface glycosaminoglycans.

  1993cited by this paper
• Affinity and kinetic analysis of the interaction of the cell adhesion molecules rat CD2 and CD48.

  1993cited by this paper
• Function of CD48 and its regulation by Epstein-Barr virus.

  1993cited by this paper
• CD48 is a low affinity ligand for human CD2.

  1993cited by this paper
• Substrate specificity of the heparin lyases from Flavobacterium heparinum.

  1993cited by this paper
• CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation

  1992cited by this paper
• Proteoglycans: many forms and many functions

  1992cited by this paper
• Animal cell mutants defective in heparan sulfate polymerization.

  1992cited by this paper
• Genetic analysis of proteoglycan structure, function and metabolism.

  1991cited by this paper
• Expression of the Blast-1 activation/adhesion molecule and its identification as CD48.

  1991cited by this paper
• Syndecan, a developmentally regulated cell surface proteoglycan that binds extracellular matrix and growth factors.

  1990cited by this paper
• Mapping the dextran sulfate binding site on CD2

  1990cited by this paper
• Dextran sulfate and heparin interact with CD4 molecules to inhibit the binding of coat protein (gp120) of HIV.

  1989cited by this paper
• Identification of a heparin binding domain of the neural cell adhesion molecule N-CAM using synthetic peptides.

  1989cited by this paper
• Molecular Modeling of Protein‐Glycosaminoglycan Interactions

  1989cited by this paper
• Molecular polymorphism of a cell surface proteoglycan: distinct structures on simple and stratified epithelia.

  1988cited by this paper
• Molecular cloning of the lymphocyte activation marker Blast‐1.

  1987cited by this paper
• Structure and function of heparan sulphate proteoglycans.

  1986cited by this paper
• Epstein-Barr virus superinduces a new human B cell differentiation antigen (B-LAST 1) expressed on transformed lymphoblasts.

  1982cited by this paper

• The Interaction between CD48 with Ligands and the Research Progress in Diseases

  2024cites this paper
• From Cancer to COVID‐19: A Perspective on Targeting Heparan Sulfate‐Protein Interactions

  2021cites this paper
• 2B4 and CD48: A powerful couple of the immune system.

  2019cites this paper
• Giardia trophozoite-secreted proteins and their effects on intestinal epithelia

  2014cites this paper
• Glycosaminoglycans in infectious disease

  2013cites this paper
• Epstein-Barr Virus Infection of Polarized Epithelial Cells via the Basolateral Surface by Memory B Cell-Mediated Transfer Infection

  2011cites this paper
• Potential therapeutic application of chondroitin sulfate/dermatan sulfate.

  2008cites this paper
• CD48 is critically involved in allergic eosinophilic airway inflammation.

  2007cites this paper
• Proteomic analysis of human immunodeficiency virus using liquid chromatography/tandem mass spectrometry effectively distinguishes specific incorporated host proteins.

  2006cites this paper
• CD48 Is an Allergen and IL-3-Induced Activation Molecule on Eosinophils1

  2006cites this paper
• The preparation and characterization of a heparin-derived oligosaccharide that binds to herpes simplex virus type 1 glycoprotein D

  2006cites this paper
• This information is current as Activation Molecule on Eosinophils CD 48 Is an Allergen and IL-3-Induced

  2006cites this paper
• Amplification of autoimmune disease by infection

  2005cites this paper
• Review Amplification of autoimmune disease by infection

  2005cites this paper
• Membrane-Associated Heparan Sulfate Proteoglycans Are Involved in the Recognition of Cellular Targets by NKp30 and NKp461

  2004cites this paper
• Pentosanpolysulfate (Elmiron) is a potent inhibitor of mast cell histamine secretion.

  2003cites this paper
• Positive regulation of GABA(B) receptors dually coupled to cyclic AMP by the allosteric agent CGP7930.

  2003cites this paper
• Characterisation of alpha-1 giardin: an immunodominant Giardia lamblia annexin with glycosaminoglycan-binding activity.

  2003cites this paper
• Cell surface heparan sulfate and its roles in assisting viral infections

  2002cites this paper
• EBV Persistence in Vivo

  2002cites this paper
• I-type lectins.

  2002cites this paper
• I-type lectins.

  2002cites this paper
• Optimal Inhibition of X4 HIV Isolates by the CXC Chemokine Stromal Cell-derived Factor 1α Requires Interaction with Cell Surface Heparan Sulfate Proteoglycans*

  2001cites this paper
• 2B4 (CD244) and CS1: novel members of the CD2 subset of the immunoglobulin superfamily molecules expressed on natural killer cells and other leukocytes

  2001cites this paper
• Chondroitin sulphate inhibits connective tissue mast cells

  2000cites this paper
• Pentosanpolysulfate inhibits mast cell histamine secretion and intracellular calcium ion levels: an alternative explanation of its beneficial effect in interstitial cystitis.

  2000cites this paper
• The ins and outs of EBV infection.

  2000cites this paper
• The interaction of heparin sulfate and adeno-associated virus 2.

  2000cites this paper
• Cell-surface heparan sulfate facilitates human immunodeficiency virus Type 1 entry into some cell lines but not primary lymphocytes.

  1999cites this paper
• The mast cell tumor necrosis factor alpha response to FimH-expressing Escherichia coli is mediated by the glycosylphosphatidylinositol-anchored molecule CD48.

  1999cites this paper