BVAN08 enhances radiosensitivity via downregulation of DNA-PKcs towards hepatic tumor xenograft

Abstract Objective To study the effects of the novel vanillin derivative BVAN08 on the radiosensitivity of hepatic cancer cells for the purpose of providing evidence for its potential use as a potential radiosensitive drug. Methods Hepatic cancer Huh-7 ​cells labeled with luciferase were used to investigate the radiosensitivity induced by BVAN08 in vivo. Colony formation assays and flow cytometry were used to measure the apoptosis and radiosensitivity of Huh-7 ​cells in vitro produced by BVAN08. Histology and immunohistochemistry were used to evaluate the toxicity of BVAN08 in vivo. Results BVAN08 induced apoptosis and cell cycle arrest of Huh-7 ​cells in a time-dependent manner. Moreover, BVAN08 combined with radiation increased the sensitivity of Huh-7 ​cells to γ-ray radiation and significantly inhibited tumor growth in vivo. The tumor inhibition rates of the BVAN08 treatment group, irradiation treatment group, and combined therapy group were 58%, 38%, and 85%, respectively. The DNA-PKcs expression in tumor tissues of the BVAN08 treatment group was lower than that of the control group (P ​ Conclusions As a natural food additive derivative, BVAN08 possesses the potential to be used as an effective anti-cancer drug to increase cell sensitivity to radiotherapy.

• Publication year

  2020

• Venue

  Unknown venue

• Publication date

  2020-08-15

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.radmp.2020.08.002
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Targeting DNA-dependent protein kinase sensitizes hepatocellular carcinoma cells to proton beam irradiation through apoptosis induction

  2019cited by this paper
• The vanillin derivative VND3207 protects intestine against radiation injury by modulating p53/NOXA signaling pathway and restoring the balance of gut microbiota.

  2019cited by this paper
• Longitudinal brain structural alterations in patients with nasopharyngeal carcinoma early after radiotherapy

  2018cited by this paper
• The Effect of Melatonin on Superoxide Dismutase and Glutathione Peroxidase Activity, and Malondialdehyde Levels in the Targeted and the Non-targeted Lung and Heart Tissues after Irradiation in Xenograft Mice Colon Cancer.

  2018cited by this paper
• Evidence of vanillin binding to CAMKIV explains the anti-cancer mechanism in human hepatic carcinoma and neuroblastoma cells

  2017cited by this paper
• Inhibiting DNA-PKcs in a non-homologous end-joining pathway in response to DNA double-strand breaks

  2017cited by this paper
• Prognostic value of DNA repair based stratification of hepatocellular carcinoma

  2016cited by this paper
• NU7441 Enhances the Radiosensitivity of Liver Cancer Cells

  2016cited by this paper
• Knocking Down Nucleolin Expression Enhances the Radiosensitivity of Non-Small Cell Lung Cancer by Influencing DNA-PKcs Activity.

  2015cited by this paper
• Inhibition of DNA-PKcs enhances radiosensitivity and increases the levels of ATM and ATR in NSCLC cells exposed to carbon ion irradiation

  2015cited by this paper
• Late Complications after Proctectomy in Rectal Cancer Patients who Underwent Radiotherapy

  2014cited by this paper
• Involvement of DNA Damage Response Pathways in Hepatocellular Carcinoma

  2014cited by this paper
• DNA-PKcs Inhibition Sensitizes Cancer Cells to Carbon-Ion Irradiation via Telomere Capping Disruption

  2013cited by this paper
• Proteomic profiling revealed the functional networks associated with mitotic catastrophe of HepG2 hepatoma cells induced by 6-bromine-5-hydroxy-4-methoxybenzaldehyde.

  2011cited by this paper
• Loss of spindle assembly checkpoint–mediated inhibition of Cdc20 promotes tumorigenesis in mice

  2009cited by this paper
• DNA-dependent protein kinase catalytic subunit modulates the stability of c-Myc oncoprotein

  2008cited by this paper
• Analysis of the retinal gene expression profile after hypoxic preconditioning identifies candidate genes for neuroprotection

  2008cited by this paper
• Imaging in the era of molecular oncology

  2008cited by this paper
• Induction of apoptosis and autophagic cell death by the vanillin derivative 6-bromine-5-hydroxy-4-methoxybenzaldehyde is accompanied by the cleavage of DNA-PKcs and rapid destruction of c-Myc oncoprotein in HepG2 cells.

  2007cited by this paper
• Vanillin derivative 6-bromine-5-hydroxy-4-methoxybenzaldehyde-elicited apoptosis and G2/M arrest of Jurkat cells proceeds concurrently with DNA-PKcs cleavage and Akt inactivation.

  2006cited by this paper
• The life and death of DNA-PK

  2005cited by this paper
• Antitumour imidazoacridone C-1311 induces cell death by mitotic catastrophe in human colon carcinoma cells.

  2005cited by this paper
• Salvage chemotherapy with a combination of paclitaxel, ifosfamide, and cisplatin for the patients with recurrent carcinoma of the uterine cervix

  2005cited by this paper
• FTY720 induces apoptosis of human hepatoma cell lines through PI3-K-mediated Akt dephosphorylation.

  2004cited by this paper
• A Sense of Danger from Radiation1

  2004cited by this paper
• Enhanced cell killing induced by the combination of radiation and the heat shock protein 90 inhibitor 17-allylamino-17- demethoxygeldanamycin: a multitarget approach to radiosensitization.

  2003cited by this paper
• Toward maintaining the genome: DNA damage and replication checkpoints.

  2002cited by this paper
• Irinotecan and radiation in combined-modality therapy for solid tumors.

  2001cited by this paper

• No citing papers are available for this paper.