Isolation and Characterization of Cellulose Degrading Bacteria from Saliva of ‘Jaffrabadi' Breed of Buffalo

Microorganisms with cellulolytic activity are considered as potential candidates in the processing of cellulosic biomass for biotechnological applications. This study was conducted to isolate and characterize cellulosedegrading bacteria collected from the saliva of ‘Jaffrabadi’ breed of buffalo by morphological and biochemical analysis. The cellulolytic bacteria were isolated and purified on CMC (Carboxy methyl cellulose) agar. Bacterial isolate produced a clear transparent zone when stained with Congo red, confirming cellulase activity. Morphological examination revealed Grampositive, rod or bacillishaped, non-motile, and spore forming nature of bacterial isolate. Different biochemical tests suggested that bacterial isolate was Bacillus subtilis. The cellulase enzyme was partially purified by (NH4)2SO4 and studied using CMCase assay. The activity of partially purified cellulose was higher (8μg/ml/min) as compared to that of crude supernatant (5.5μg/ml/min). The optimum pH and optimum temperature range were found to be 6.5 7.5 and 40 – 50 °C. Protein profiling using 10% SDS-PAGE further confirmed the purity of the partially purified sample.

• Publication year

  2020

• Venue

  International Journal of Livestock Research

• Publication date

  Unknown publication date

• Fields of study

  Biology, Agricultural and Food Sciences, Environmental Science

• Identifiers
  DOI 10.5455/ijlr.20200509115503
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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