Visualization of Endothelial Cell Damage Caused by Ultrasonically Induced Microbubble Oscillation Inside a Capillary Phantom

Mechanisms of BBB opening were investigated using a capillary model created by a three-dimensional cell culture technique. The model was made using human umbilical vein endothelial cells (HUVECs) suspended in collagen gel. Incubation with endothelial growth factor for one week resulted in the creation of capillary lumens, and a suspension of microbubbles supplemented with FITC-dextran, SYTOX Blue, and Calcein-AM was introduced inside the lumens. Bubble-cell interaction under exposure to short pulsed ultrasound of 1.0 MHz in center frequency and 0.4 MPa in peak negative pressure was captured using a high-speed camera, and lumen and endothelial cell damage was visualized using fluorescence microscopy. High-speed observation showed that non-uniform contraction of a bubble caused significant local deformation of the lumen beside the bubble, and strong extravasation from the lumen (FITC-dextran) and significant membrane damage of the endothelial cells (SYTOX Blue) were confirmed at this location. Lethal damage was also observed frequently (Calcein). On the other hand, cells on the opposite side of the lumen received stretching force during bubble expansion, and smaller extravasation and cell damage maintained the integrity of endothelial cells. These suggested the presence of different mechanisms of BBB opening.

• Publication year

  2020

• Venue

  IUS

• Publication date

  2020-09-07

• Fields of study

  Medicine, Chemistry, Engineering

• Identifiers
  DOI 10.1109/IUS46767.2020.9251785
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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