MAPKKK7 from Plasmodiophora brassicae regulates low-light-dependent Nicotiana benthamiana immunity.

MAPKKK is the largest family of mitogen-activated protein kinase (MAPK) cascade, which is known to play important roles in plant pathogen interaction by regulating fungal cell proliferation, growth and pathogenicity. So far, only a few of them have been characterized due to the functional redundancy of MAPKKKs. In this study, it is interesting that PbMAPKKK7, a MAPKKK from Plasmodiophora brassicae, was clustered into the A3 subgroup of plant MAPKKKs by a phylogenetic analysis and BCK1 and STE group of fungal MAPKKKs. The function of PbMAPKKK7 in accumulation of reactive oxygen species (ROS) and cell death in Nicotiana benthamiana were characterized. Agroinfiltrated with PbMAPKKK7 mutated protein kinase domain relieved the symptoms. Interestingly, the induction of cell death was dependent on the light intensity. Transcriptional profiling analysis demonstrated that PbMAPKKK7 highly expressed during the cortex infection stages, indicating its important role in P. brassicae infection. These functional analyses of PbMAPKKK7 would build our knowledge of new roles of the MAPK cascade pathway in N. benthamiana and P. brassicae interactions.

• Publication year

  2020

• Venue

  Phytopathology

• Publication date

  2020-12-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1094/PHYTO-08-20-0323-RPMID 33258412
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• MAPK pathway-related tyrosine phosphatases regulate development, secondary metabolism and pathogenicity in fungus Aspergillus flavus.

  2020cited by this paper
• The RasGEF FgCdc25 regulates fungal development and virulence in Fusarium graminearum via cAMP and MAPK signaling pathways.

  2020cited by this paper
• Identification and Characterization of Plasmodiophora brassicae Primary Infection Effector Candidates that Suppress or Induce Cell Death in Host and Nonhost Plants.

  2019cited by this paper
• Arabidopsis thaliana expressing PbBSMT, a gene encoding a SABATH-type methyltransferase from the plant pathogenic protist Plasmodiophora brassicae, show leaf chlorosis and altered host susceptibility.

  2019cited by this paper
• Phenotypic and Genetic Characterization of Botrytis cinerea Population from Kiwifruit in Sichuan Province, China.

  2019cited by this paper
• Characterization of Five Molecular Markers for Pathotype Identification of the Clubroot Pathogen Plasmodiophora brassicae.

  2018cited by this paper
• Silencing PsKPP4, a MAP kinase kinase kinase gene, reduces pathogenicity of the stripe rust fungus.

  2018cited by this paper
• MAPKK Inhibitor U0126 Inhibits Plasmodiophora brassicae Development.

  2018cited by this paper
• The MAPKKK CgMck1 Is Required for Cell Wall Integrity, Appressorium Development, and Pathogenicity in Colletotrichum gloeosporioides

  2018cited by this paper
• Mitogen-Activated Protein Kinase Cascades in Plant Hormone Signaling

  2018cited by this paper
• ModelFinder: Fast Model Selection for Accurate Phylogenetic Estimates

  2017cited by this paper
• A candidate RxLR effector from Plasmopara viticola can elicit immune responses in Nicotiana benthamiana

  2017cited by this paper
• The compact genome of the plant pathogen Plasmodiophora brassicae is adapted to intracellular interactions with host Brassica spp

  2016cited by this paper
• Apoplastic recognition of multiple candidate effectors from the wheat pathogen Zymoseptoria tritici in the nonhost plant Nicotiana benthamiana

  2016cited by this paper
• The Plasmodiophora brassicae genome reveals insights in its life cycle and ancestry of chitin synthases

  2015cited by this paper
• Immune activation mediated by the late blight resistance protein R1 requires nuclear localization of R1 and the effector AVR1.

  2015cited by this paper
• Mitogen-activated protein kinase kinase kinase (MAPKKK) 4 from rapeseed (Brassica napus L.) is a novel member inducing ROS accumulation and cell death.

  2015cited by this paper
• Identification and functional analysis of mitogen-activated protein kinase kinase kinase (MAPKKK) genes in canola (Brassica napus L.)

  2014cited by this paper
• IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies

  2014cited by this paper
• The Clubroot Pathogen (Plasmodiophora brassicae) Influences Auxin Signaling to Regulate Auxin Homeostasis in Arabidopsis

  2013cited by this paper
• MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability

  2013cited by this paper
• The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1

  2012cited by this paper
• MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space

  2012cited by this paper
• Plasmodiophora brassicae: a review of an emerging pathogen of the Canadian canola (Brassica napus) crop.

  2012cited by this paper
• Identification of three MAPKKKs forming a linear signaling pathway leading to programmed cell death in Nicotiana benthamiana

  2012cited by this paper
• Phosphorylation of Serine 114 on Atg32 mediates mitophagy

  2011cited by this paper
• Tomato 14-3-3 Protein TFT7 Interacts with a MAP Kinase Kinase to Regulate Immunity-associated Programmed Cell Death Mediated by Diverse Disease Resistance Proteins*

  2011cited by this paper
• Two Host Cytoplasmic Effectors Are Required for Pathogenesis of Phytophthora sojae by Suppression of Host Defenses1[W][OA]

  2010cited by this paper
• Identification and Distribution of Soybean mosaic virus Strains in Southern China.

  2010cited by this paper
• Tomato MAPKKKε is a positive regulator of cell-death signaling networks associated with plant immunity.

  2010cited by this paper
• HINKEL kinesin, ANP MAPKKKs and MKK6/ANQ MAPKK, which phosphorylates and activates MPK4 MAPK, constitute a pathway that is required for cytokinesis in Arabidopsis thaliana

  2010cited by this paper
• The MAPKK kinase ChSte11 regulates sexual/asexual development, melanization, pathogenicity, and adaptation to oxidative stress in Cochliobolus heterostrophus

  2009cited by this paper
• Transcriptional adaptation of Mycosphaerella graminicola to programmed cell death (PCD) of its susceptible wheat host.

  2007cited by this paper
• Ancient signals: comparative genomics of plant MAPK and MAPKK gene families.

  2006cited by this paper
• Localization of Ptr ToxA Produced by Pyrenophora tritici-repentis Reveals Protein Import into Wheat Mesophyll Cells

  2005cited by this paper
• MAPKKKα is a positive regulator of cell death associated with both plant immunity and disease

  2004cited by this paper
• NPR1 Modulates Cross-Talk between Salicylate- and Jasmonate-Dependent Defense Pathways through a Novel Function in the Cytosol Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.009159.

  2003cited by this paper
• An Arabidopsis Mitogen-Activated Protein Kinase Kinase Kinase Gene Family Encodes Essential Positive Regulators of Cytokinesis Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.001164.

  2002cited by this paper
• NPK1, an MEKK1-like mitogen-activated protein kinase kinase kinase, regulates innate immunity and development in plants.

  2002cited by this paper
• Mitogen-activated protein kinase cascades in plants: a new nomenclature.

  2002cited by this paper
• Mammalian MAP kinase signalling cascades

  2001cited by this paper
• Suppression of auxin signal transduction by a MAPK cascade in higher plants

  1998cited by this paper
• Possible involvement of differential splicing in regulation of the activity of Arabidopsis ANP1 that is related to mitogen-activated protein kinase kinase kinases (MAPKKKs).

  1997cited by this paper
• MAP kinase kinase kinase, MAP kinase kinase and MAP kinase.

  1994cited by this paper

• The conserved GOLD domain in the Plasmodiophora brassicae effector Pb257 is required for triggering cell death and root swelling

  2024influential citation
• Plasmodiophora brassicae effector PbPE23 induces necrotic responses in both host and non-host plants.

  2024cites this paper
• Endomembrane-Targeting Plasmodiophora brassicae Effectors Modulate PAMP Triggered Immune Responses in Plants

  2021cites this paper