Pyrene Tags for the Detection of Carbohydrates by Label‐Assisted Laser Desorption/Ionisation Mass Spectrometry **

Matrix‐assisted laser desorption/ionisation mass spectrometry (MALDI‐MS) is widely used for the analysis of biomolecules. Label‐assisted laser desorption/ionisation mass spectrometry (LALDI‐MS) is a matrix‐free variant of MALDI‐MS, in which only analytes covalently attached to a laser desorption/ionisation (LDI) enhancer are detected. LALDI‐MS has shown promise in overcoming the limitations of MALDI‐MS in terms of sample preparation and MS analysis. In this work, we have developed a series of pyrene‐based LDI reagents (LALDI tags) that can be used for labelling and LALDI‐MS analysis of reducing carbohydrates from complex (biological) samples without the need for additional chemical derivatisation or purification. We have systematically explored the suitability of four pyrene‐based LDI enhancers and three aldehyde‐reactive handles, optimised sample preparation, and demonstrated the use of LALDI tags for the detection of lactose. We have also exemplified the potential of LALDI tags for labelling carbohydrates in biological samples by direct detection of lactose in cow's milk. These results demonstrate that LALDI‐MS is a promising technique for the analysis of reducing carbohydrates in biological samples, and pave the way for the development of LALDI‐MS for glycomics and diagnostics.

• Publication year

  2020

• Venue

  ChemBioChem

• Publication date

  2020-12-09

• Fields of study

  Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1002/cbic.202000721PMID 33296552
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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