Post-transcriptional modifications modulate conformational dynamics in human U2–U6 snRNA complex

From yeast to humans, all spliceosomal snRNAs share structural similarities, but higher eukaryotes contain numerous post-transcriptional modifications with undefined roles. Using single-molecule fluorescence we show that several post-transcriptional modifications in human U2 snRNA modulate the dynamic equilibrium of a model U2–U6 complex by stabilizing the four-helix structure. The small magnitude of this effect, however, suggests a primary role in specific RNA–protein interaction mediation in vivo.

• Publication year

  2014

• Venue

  RNA: A publication of the RNA Society

• Publication date

  2014-01-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1261/rna.041806.113PMID 24243115PMCID 3866641
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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