Identification and characterization of a novel multi-stress responsive gene in Arabidopsis

Abiotic stresses especially salinity, drought and high temperature result in considerable reduction of crop productivity. In this study, we identified AT4G18280 annotated as a glycine-rich cell wall protein-like (hereafter refer to as GRPL1) protein as a potential multistress-responsive gene. Analysis of public transcriptome data and GUS assay of pGRPL1::GUS showed a strong induction of GRPL1 under drought, salinity and heat stresses. Transgenic plants overexpressing GRPL1-3HA showed significantly higher germination, root elongation and survival rate under salt stress. Moreover, the 35S::GRPL1-3HA transgenic lines also showed higher survival rates under drought and heat stresses. GRPL1 showed similar expression patterns with Abscisic acid (ABA)-pathway genes under different growth and stress conditions, suggesting a possibility that GRPL1 might act in the ABA pathway that is further supported by the inability of ABA-deficient mutant (aba2-1) to induce GRPL1 under drought stress. Taken together, our data presents GRPL1 as a potential multi-stress responsive gene working downstream of ABA.

• Publication year

  2020

• Venue

  PLoS ONE

• Publication date

  2020-12-17

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1371/journal.pone.0244030PMID 33332435PMCID 7746274
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular cloning and functional analysis of lotus salt-induced NnDREB2C, NnPIP1-2 and NnPIP2-1 in Arabidopsis thaliana

  2019cited by this paper
• Plant Glycine-Rich Proteins in Stress Response: An Emerging, Still Prospective Story

  2018cited by this paper
• A novel cotton WRKY gene, GhWRKY6-like, improves salt tolerance by activating the ABA signaling pathway and scavenging of reactive oxygen species.

  2018cited by this paper
• Salt stress-induced FERROCHELATASE 1 improves resistance to salt stress by limiting sodium accumulation in Arabidopsis thaliana

  2017cited by this paper
• Generalized Mechanism of Field Emission from Nanostructured Semiconductor Film Cathodes

  2017cited by this paper
• Abscisic Acid Signaling and Abiotic Stress Tolerance in Plants: A Review on Current Knowledge and Future Prospects

  2017cited by this paper
• Application of Plant-Growth-Promoting Fungi Trichoderma longibrachiatum T6 Enhances Tolerance of Wheat to Salt Stress through Improvement of Antioxidative Defense System and Gene Expression

  2016cited by this paper
• Proline Coordination with Fatty Acid Synthesis and Redox Metabolism of Chloroplast and Mitochondria1[OPEN]

  2016cited by this paper
• Proline Accumulation in Plants: Roles in Stress Tolerance and Plant Development

  2016cited by this paper
• Salt Stress Affects the Redox Status of Arabidopsis Root Meristems

  2016cited by this paper
• Abiotic Stress Signaling and Responses in Plants.

  2016cited by this paper
• ABA Is Required for Plant Acclimation to a Combination of Salt and Heat Stress

  2016cited by this paper
• A New Insight of Salt Stress Signaling in Plant

  2016cited by this paper
• Increased tolerance to salt stress in OPDA-deficient rice ALLENE OXIDE CYCLASE mutants is linked to an increased ROS-scavenging activity

  2015cited by this paper
• Overexpression of a Novel Component Induces HAK5 and Enhances Growth in Arabidopsis

  2014cited by this paper
• Expression of Arabidopsis glycine-rich RNA-binding protein AtGRP2 or AtGRP7 improves grain yield of rice (Oryza sativa) under drought stress conditions.

  2014influential reference
• Genome-wide analysis of alternative splicing of pre-mRNA under salt stress in Arabidopsis

  2014cited by this paper
• bHLH122 is important for drought and osmotic stress resistance in Arabidopsis and in the repression of ABA catabolism.

  2014cited by this paper
• Gene expression profiling of soybean leaves and roots under salt, saline-alkali and drought stress by high-throughput Illumina sequencing.

  2013cited by this paper
• Genome expression profile analysis reveals important transcripts in maize roots responding to the stress of heavy metal Pb.

  2013cited by this paper
• Transcriptional responses to drought stress in root and leaf of chickpea seedling

  2012cited by this paper
• Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks

  2012cited by this paper
• De novo sequencing and analysis of root transcriptome using 454 pyrosequencing to discover putative genes associated with drought tolerance in Ammopiptanthus mongolicus

  2012cited by this paper
• Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions

  2012cited by this paper
• Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stress tolerance in transgenic Arabidopsis plants.

  2012cited by this paper
• Transcriptome Profiling of Dehydration Stress in the Chinese Cabbage (Brassica rapa L. ssp. pekinensis) by Tag Sequencing

  2012cited by this paper
• Transcriptome analyses of Bactericera cockerelli adults in response to “Candidatus Liberibacter solanacearum” infection

  2012cited by this paper
• Achievements and challenges in understanding plant abiotic stress responses and tolerance.

  2011cited by this paper
• Histostaining for Tissue Expression Pattern of Promoter-driven GUS Activity in Arabidopsis

  2011cited by this paper
• Implications of climate change for diseases, crop yields and food security

  2011cited by this paper
• Climate Trends and Global Crop Production Since 1980

  2011cited by this paper
• Glycine-rich RNA-binding proteins are functionally conserved in Arabidopsis thaliana and Oryza sativa during cold adaptation process

  2010cited by this paper
• Molecular Basis of the Core Regulatory Network in ABA Responses: Sensing, Signaling and Transport

  2010cited by this paper
• Genome-wide mapping of alternative splicing in Arabidopsis thaliana.

  2010cited by this paper
• Radically Rethinking Agriculture for the 21st Century

  2010cited by this paper
• Salicylic acid promotes seed germination under high salinity by modulating antioxidant activity in Arabidopsis.

  2010cited by this paper
• Growth, yield, water status and ionic distribution response of three bambara groundnut (Vigna subterranea (L.) Verdc.) landraces grown under saline conditions.

  2010cited by this paper
• Research on plant abiotic stress responses in the post-genome era: past, present and future.

  2010cited by this paper
• Methods for determination of proline in plants.

  2010cited by this paper
• Stress-induced changes in the Arabidopsis thaliana transcriptome analyzed using whole-genome tiling arrays.

  2009cited by this paper
• Mechanisms of salinity tolerance.

  2008influential reference
• Reductions in maize root-tip elongation by salt and osmotic stress do not correlate with apoplastic O2*- levels.

  2008cited by this paper
• Gibberellic acid-mediated salt signaling in seed germination

  2008cited by this paper
• Genetic approaches to crop improvement: responding to environmental and population changes

  2008cited by this paper
• Durum wheat genes up-regulated in the early phases of cold stress are modulated by drought in a developmental and genotype dependent manner

  2007cited by this paper
• Perception and transduction of abscisic acid signals: keys to the function of the versatile plant hormone ABA.

  2007cited by this paper
• The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars

  2007cited by this paper
• Effect of NaCl on Stomatal Resistance and Proline, Chlorophyll, Na, Cl and K Concentrations of Lentil Plants

  2007cited by this paper
• Reactive Oxygen Species in Plant Cell Death1

  2006cited by this paper
• Cold, salinity and drought stresses: an overview.

  2005cited by this paper
• Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance

  2003cited by this paper
• Salinity Tolerance of Phaseolus Species during Germination and Early Seedling Growth

  2002cited by this paper
• Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves.

  2002cited by this paper
• Effect of salinity and temperature on the germination of Urochondra setulosa (Trin.) C. E. Hubbard

  2001cited by this paper
• Expression of cell cycle regulatory genes and morphological alterations in response to salt stress in Arabidopsis thaliana

  2000cited by this paper
• Plant glycine-rich proteins: a family or just proteins with a common motif?

  2000cited by this paper
• THE FUNCTION AND METABOLISM OF ASCORBIC ACID IN PLANTS

  1996cited by this paper
• Arabidopsis mutants with reduced response to NaCl and osmotic stress

  1995cited by this paper
• The Role of Cell Wall Adjustments in Plant Resistance to Water Deficits

  1995cited by this paper
• Salt tolerance of Hordeum and Brassica species during germination and early seedling growth

  1995cited by this paper
• Rapid determination of free proline for water-stress studies

  1973cited by this paper
• COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

  1949cited by this paper

• Integrative cross-platform transcriptomic analysis coupled with machine learning identifies candidate biomarkers of abiotic stress resilience in Arabidopsis thaliana.

  2026cites this paper
• Plant-Based Solutions for Non-Productive Sites Useful in the Management of Dry Land

  2023cites this paper
• Meta-Analysis as a Tool to Identify Candidate Genes Involved in the Fagus sylvatica L. Abiotic Stress Response

  2022cites this paper
• Effect of Drought Stress on Degradation and Remodeling of Membrane Lipids in Nostoc flagelliforme

  2022cites this paper
• The receptor kinase SRF3 coordinates iron-level and flagellin dependent defense and growth responses in plants

  2021cites this paper