Structural stability and unfolding properties of thermostable bacterial alpha-amylases: a comparative study of homologous enzymes.

No abstract is available for this paper.

• Publication year

  2004

• Venue

  Biochemistry

• Publication date

  2004-07-07

• Fields of study

  Biology, Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1021/BI0493362PMID 15274613
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Adaptation of Class‐13 α‐Amylases to Diverse Living Conditions

  2004cited by this paper
• Adaptation of class-13 alpha-amylases to diverse living conditions.

  2004cited by this paper
• Loops, linkages, rings, catenanes, cages, and crowders: entropy-based strategies for stabilizing proteins.

  2004cited by this paper
• Some like it cold: biocatalysis at low temperatures.

  2004cited by this paper
• Structural and Functional Adaptations to Extreme Temperatures in Psychrophilic, Mesophilic, and Thermophilic DNA Ligases*

  2003cited by this paper
• A measure of conformational entropy change during thermal protein unfolding using neutron spectroscopy.

  2003cited by this paper
• Role of residual structure in the unfolded state of a thermophilic protein

  2003cited by this paper
• Activity-Stability Relationships in Extremophilic Enzymes*

  2003cited by this paper
• High thermal and chemical stability of Thermus thermophilus seven-iron ferredoxin. Linear clusters form at high pH on polypeptide unfolding.

  2003cited by this paper
• Effect of calcium ions on the irreversible denaturation of a recombinant Bacillus halmapalus alpha-amylase: a calorimetric investigation.

  2003cited by this paper
• Kinetic Stabilization of Bacillus licheniformis α-Amylase through Introduction of Hydrophobic Residues at the Surface*

  2003cited by this paper
• Activity, stability and flexibility in glycosidases adapted to extreme thermal environments.

  2003cited by this paper
• Long-Range Interactions Within a Nonnative Protein

  2002cited by this paper
• Pyrococcus furiosus α-Amylase Is Stabilized by Calcium and Zinc†

  2002cited by this paper
• Comparison of the folding processes of T. thermophilus and E. coli ribonucleases H.

  2002cited by this paper
• Pyrococcus furiosus alpha-amylase is stabilized by calcium and zinc.

  2002cited by this paper
• Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability

  2001cited by this paper
• Activity and Stability of a Thermostable α-Amylase Compared to Its Mesophilic Homologue: Mechanisms of Thermal Adaptation†

  2001cited by this paper
• Activity and stability of a thermostable alpha-amylase compared to its mesophilic homologue: mechanisms of thermal adaptation.

  2001cited by this paper
• Dynamical properties of α-amylase in the folded and unfolded state: the role of thermal equilibrium fluctuations for conformational entropy and protein stabilisation

  2001cited by this paper
• High thermal stability of 3-isopropylmalate dehydrogenase from Thermus thermophilus resulting from low DeltaC(p) of unfolding.

  2001cited by this paper
• Chemical modification of bacterial alpha-amylases: changes in tertiary structures and the effect of additional calcium.

  2001cited by this paper
• Persistence of Native-Like Topology in a Denatured Protein in 8 M Urea

  2001cited by this paper
• Molecular confinement influences protein structure and enhances thermal protein stability

  2001cited by this paper
• Comparative analyses of the conformational stability of a hyperthermophilic protein and its mesophilic counterpart.

  2001cited by this paper
• Distributions of structural features contributing to thermostability in mesophilic and thermophilic α/β barrel glycosyl hydrolases

  2000cited by this paper
• Protein engineering of bacterial α-amylases

  2000cited by this paper
• Distributions of structural features contributing to thermostability in mesophilic and thermophilic alpha/beta barrel glycosyl hydrolases.

  2000cited by this paper
• Tryptophan phosphorescence study of enzyme flexibility and unfolding in laboratory-evolved thermostable esterases.

  2000cited by this paper
• Directed evolution study of temperature adaptation in a psychrophilic enzyme.

  2000cited by this paper
• Structural Equilibrium Fluctuations in Mesophilic and Thermophilic α-Amylase

  2000cited by this paper
• From the Cover: Millisecond time scale conformational flexibility in a hyperthermophile protein at ambient temperature

  2000cited by this paper
• Two exposed amino acid residues confer thermostability on a cold shock protein

  2000cited by this paper
• Protein engineering of bacterial alpha-amylases.

  2000cited by this paper
• Structural equilibrium fluctuations in mesophilic and thermophilic alpha-amylase.

  2000cited by this paper
• Structural analysis of a chimeric bacterial alpha-amylase. High-resolution analysis of native and ligand complexes.

  2000cited by this paper
• Thermodynamic stability of a cold-active alpha-amylase from the Antarctic bacterium Alteromonas haloplanctis.

  1999influential reference
• Hydrodynamic radii of native and denatured proteins measured by pulse field gradient NMR techniques.

  1999cited by this paper
• Comparing the thermodynamic stabilities of a related thermophilic and mesophilic enzyme.

  1999cited by this paper
• Proteins from hyperthermophiles: stability and enzymatic catalysis close to the boiling point of water.

  1998cited by this paper
• Improved thermostability of a Bacillus alpha-amylase by deletion of an arginine-glycine residue is caused by enhanced calcium binding.

  1998cited by this paper
• Kinetic role of electrostatic interactions in the unfolding of hyperthermophilic and mesophilic rubredoxins.

  1998cited by this paper
• Adjustment of conformational flexibility is a key event in the thermal adaptation of proteins.

  1998influential reference
• Denatured states of yeast phosphoglycerate kinase.

  1998cited by this paper
• Crystal structures of Escherichia coli and Salmonella typhimurium 3-isopropylmalate dehydrogenase and comparison with their thermophilic counterpart from Thermus thermophilus.

  1997cited by this paper
• Extremophiles.

  1997cited by this paper
• Protein structure : a practical approach

  1997cited by this paper
• Structure and stability of hyperstable proteins: glycolytic enzymes from hyperthermophilic bacterium Thermotoga maritima.

  1996cited by this paper
• Crystal structure of calcium-depleted Bacillus licheniformis alpha-amylase at 2.2 A resolution.

  1995influential reference
• Kinetic study of the irreversible thermal denaturation of Bacillus licheniformis alpha-amylase.

  1989cited by this paper
• Microbial amylolytic enzymes.

  1989cited by this paper
• Protein stability curves

  1987cited by this paper
• CONTIN: A general purpose constrained regularization program for inverting noisy linear algebraic and integral equations

  1984cited by this paper
• Principles of fluorescence spectroscopy

  1983cited by this paper
• Exposure of tryptophanyl residues in proteins. Quantitative determination by fluorescence quenching studies.

  1976cited by this paper
• Temperature as a selective factor in protein evolution: the adaptational strategy of "compromise".

  1975cited by this paper
• Principles that govern the folding of protein chains.

  1973cited by this paper
• The preparation of guanidine hydrochloride.

  1972cited by this paper
• Vapor pressure osmometry.

  1972cited by this paper
• Protein denaturation.

  1968cited by this paper
• Viscosity and density of aqueous solutions of urea and guanidine hydrochloride.

  1966cited by this paper

• Deciphering Unfolding Pathways: Chemical, Heat, and Acid Induced Investigations using Fenugreek (Trigonella foenum-graecum) α-Amylase

  2024cites this paper
• Amino acids and glycine derivatives differently affect refolding of mesophilic and thermophilic like α-amylases: implications in protein refolding and aggregation

  2024cites this paper
• Improvement in salt-tolerance of Aspergillus oryzae γ-glutamyl transpeptidase via protein chimerization with Aspergillus sydowii homolog.

  2023cites this paper
• Correlating the Local and Global Dynamics of an Enzyme in the Crowded Milieu.

  2022cites this paper
• Structural and functional adaptation in extremophilic microbial α-amylases

  2022cites this paper
• A Novel Carboxylesterase Derived from a Compost Metagenome Exhibiting High Stability and Activity towards High Salinity

  2021cites this paper
• Protein Dynamics as a Sensor for Macromolecular Crowding: Insights into Mixed Crowding

  2021cites this paper
• Differential effect of polyol and sugar osmolytes on the refolding of homologous alpha amylases: A comparative study.

  2021cites this paper
• Cholesteryl-Conjugated Ribonuclease A Exhibits Enzyme Activity in Aqueous Solution and Resistance to Dimethyl Sulfoxide

  2021cites this paper
• Different unfolding pathways of homologous alpha amylases from Bacillus licheniformis (BLA) and Bacillus amyloliquefaciens (BAA) in GdmCl and urea.

  2020cites this paper
• Denaturant induced equilibrium unfolding and conformational transitional studies of germinated fenugreek β-amylase revealed molten globule like state at low pH.

  2020cites this paper
• Conserved Protein YpmR of Moderately Halophilic Bacillus licheniformis has Hydrolytic Activity on p-Nitrophenyl Laurate

  2020cites this paper
• Synthesis and Characterization of Cholesteryl Conjugated Lysozyme (CHLysozyme)

  2020cites this paper
• Bacillus licheniformis α-amylase: Structural feature in a biomimetic solution and structural changes in extrinsic conditions.

  2019cites this paper
• Biophysical insight into structure-function relation of Allium sativum Protease Inhibitor by thermal, chemical and pH-induced modulation using comprehensive spectroscopic analysis.

  2017cites this paper
• Heat-resistant cytosolic malate dehydrogenases (cMDHs) of thermophilic intertidal snails (genus Echinolittorina): protein underpinnings of tolerance to body temperatures reaching 55°C

  2017cites this paper
• Influence of Buffer Composition and Calcium Chloride on GdnHCl Denaturation of Bacillus licheniformis α-Amylase.

  2016cites this paper
• Correction: Heat, Acid and Chemically Induced Unfolding Pathways, Conformational Stability and Structure-Function Relationship in Wheat α-Amylase

  2015cites this paper
• Heat, Acid and Chemically Induced Unfolding Pathways, Conformational Stability and Structure-Function Relationship in Wheat α-Amylase

  2015cites this paper
• Improvement of the stability and activity of the BPO-A1 haloperoxidase from Streptomyces aureofaciens by directed evolution.

  2014cites this paper
• Structural Stability and Unfolding Properties of Cutinases from Thermobifida fusca

  2014cites this paper
• The effects of urea, guanidinium chloride and sorbitol on porphyrin aggregation: Molecular dynamics simulation

  2013cites this paper
• Dendrons and dendrimers as pseudochaperonins for refolding of proteins

  2013cites this paper
• Does Recovery in the Spectral Characteristics of GdnHCl-Denatured Bacillus licheniformis α-Amylase Due to Added Calcium Point towards Protein Stabilization?

  2013cites this paper
• Effect of compatible and noncompatible osmolytes on the enzymatic activity and thermal stability of bovine liver catalase

  2013cites this paper
• Thermal, Chemical and pH Induced Denaturation of a Multimeric β-Galactosidase Reveals Multiple Unfolding Pathways

  2012cites this paper
• Protease and Amylase Stability in the Presence of Chelators Used in Laundry Detergent Applications: Correlation Between Chelator Properties and Enzyme Stability in Liquid Detergents

  2012cites this paper
• Acid-Induced Formation of Molten Globule States in the Wild Type Escherichia coli 5-Enolpyruvylshikimate 3-Phosphate Synthase and its Three Mutated Forms: G96A, A183T and G96A/A183T

  2011cites this paper
• An analysis of temperature adaptation in cold active, mesophilic and thermophilic Bacillus α-amylases.

  2011cites this paper
• Conformational destabilization of Bacillus licheniformis α-amylase induced by lysine modification and calcium depletion.

  2011cites this paper
• Structural stability and unfolding transition of β-glucosidases: a comparative investigation on isozymes from a thermo-tolerant yeast

  2011cites this paper
• How do thermophilic proteins and proteomes withstand high temperature?

  2011cites this paper
• Structural stability of soybean (Glycine max) α-amylase: properties of the unfolding transition studied with fluorescence and CD spectroscopy.

  2011cites this paper
• Chitosan-bovine serum albumin complex formation: a model to design an enzyme isolation method by polyelectrolyte precipitation.

  2010cites this paper
• Hlutverk sameinda-sveigjanleika í hitastigsaðlögun subtilisín-líkra serín próteinasa

  2010cites this paper
• The effect of calcium binding on the unfolding barrier: A kinetic study on homologous alpha-amylases.

  2010cites this paper
• A chemically modified alpha-amylase with a molten-globule state has entropically driven enhanced thermal stability.

  2010cites this paper
• Calcium-induced stabilization of-amylase against guanidine hydrochloride denaturation

  2010influential citation
• Calcium-induced stabilization of -amylase against guanidine hydrochloride denaturation

  2010cites this paper
• Engineering of a truncated alpha-amylase of Bacillus sp. strain TS-23 for the simultaneous improvement of thermal and oxidative stabilities.

  2010cites this paper
• An injectable hyaluronic acid-tyramine hydrogel system for protein delivery.

  2009cites this paper
• Interaction and complex formation between catalase and cationic polyelectrolytes: chitosan and Eudragit E100.

  2009cites this paper
• Supporting Information for : Evolution of cyclodextrin product specificity of cyclodextrin glucanotransferase enzymes

  2009cites this paper
• Seeking new mutation clues from Bacillus licheniformis amylase by molecular dynamics simulations

  2009cites this paper
• How Flexible Polymers Interact with Proteins and Its Relationship with the Protein Separation Method by Protein–Polymer Complex Formation

  2009cites this paper
• Conformational stability of alpha-amylase from malted sorghum (Sorghum bicolor): reversible unfolding by denaturants.

  2009cites this paper
• Thermostable biocatalytic films of enzymes and polylysine on electrodes and nanoparticles in microemulsions.

  2008cites this paper
• Porcine pancreatic alpha amylase and its isoforms--effect of deglycosylation by peptide-N-glycosidase F.

  2008cites this paper
• Conformational stability and integrity of alpha-amylase from mung beans: evidence of kinetic intermediate in GdmCl-induced unfolding.

  2008cites this paper
• Folding control and unfolding free energy of yeast iso-1-cytochrome c bound to layered zirconium phosphate materials monitored by surface plasmon resonance.

  2008cites this paper
• Impact of Arg210-Ser211 deletion on thermostability of a truncated Bacillus sp. strain TS-23 α-amylase

  2008cites this paper
• Calorimetric studies on renaturation by CaCl2 addition of metal-free α-amylase from Bacillus Licheniformis (BLA)

  2008cites this paper
• Cereal starch digestibility by porcine pancreatic α-amylase isoforms in vitro: Effect of non-starch polysaccharides

  2008cites this paper
• Adaptation to high temperatures through macromolecular dynamics by neutron scattering

  2007cites this paper
• Reversible and irreversible unfolding of multi-domain proteins.

  2007cites this paper
• Nucleotide sequence, structural investigation and homology modeling studies of a Ca2+-independent alpha-amylase with acidic pH-profile.

  2007cites this paper
• Thermostable peroxidase-polylysine films for biocatalysis at 90 degrees C.

  2007cites this paper
• A mechanism for the stabilization of the secondary structure of a peptide by liquid ethylene glycol and its aqueous solutions

  2007cites this paper
• Effects of heme pocket structure and mobility on cytochrome c stability.

  2007cites this paper
• Biophysical characterization of the enzyme I of the Streptomyces coelicolor phosphoenolpyruvate:sugar phosphotransferase system.

  2006cites this paper
• In Silico Analysis of the Thermodynamic Stability Changes of Psychrophilic and Mesophilic alpha-Amylases upon Exhaustive Single-Site Mutations

  2006cites this paper
• Ca-binding to Bacillus licheniformis alpha-amylase (BLA).

  2006cites this paper
• Ca-binding to Bacillus licheniformis α-amylase (BLA)

  2006cites this paper
• Thermostabilization of Bacillus amyloliquefaciens alpha-amylase by chemical cross-linking.

  2006cites this paper
• Thermostabilization of Bacillus amyloliquefaciens α-amylase by chemical cross-linking

  2006cites this paper
• Interactions of barley α-amylase isozymes with Ca2 + , substrates and proteinaceous inhibitors

  2006cites this paper
• How aggregation and conformational scrambling of unfolded states govern fluorescence emission spectra.

  2006cites this paper
• Comparison of the molten globule states of thermophilic and mesophilic alpha-amylases.

  2006cites this paper
• Comparison of the molten globule states of thermophilic and mesophilic α-amylases

  2006cites this paper
• Neutron Scattering Reveals the Dynamic Basis of Protein Adaptation to Extreme Temperature*

  2005cites this paper
• Structural and dynamical features contributing to thermostability in alpha-amylases.

  2005cites this paper
• Reversible equilibrium unfolding of triosephosphate isomerase from Trypanosoma cruzi in guanidinium hydrochloride involves stable dimeric and monomeric intermediates.

  2005cites this paper
• Structural and dynamical features contributing to thermostability in α-amylases

  2005cites this paper
• Ca2+–Surfactant Interactions Affect Enzyme Stability in Detergent Solutions

  2005cites this paper
• Thermostability of Irreversible Unfolding α-Amylases Analyzed by Unfolding Kinetics*

  2005cites this paper
• EN MIKROKALORIMETRISK UNDERSØGELSE AF SOLUTTERS EFFEKT PÅ DEN TERMISK INDUCEREDE, IRREVERSIBLE DENATURERING AF BACILLUS HALMAPALUS α-AMYLASE

  2005cites this paper
• Thermostability of irreversible unfolding alpha-amylases analyzed by unfolding kinetics.

  2005cites this paper