Codon optimization, promoter and expression system selection that achieved high-level production of Yarrowia lipolytica lipase in Pichia pastoris.

No abstract is available for this paper.

• Publication year

  2015

• Venue

  Enzyme and Microbial Technology

• Publication date

  2015-04-01

• Fields of study

  Biology, Medicine, Chemistry, Engineering

• Identifiers
  DOI 10.1016/j.enzmictec.2014.10.007PMID 25765312
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Deciphering the rules by which dynamics of mRNA secondary structure affect translation efficiency in Saccharomyces cerevisiae

  2014cited by this paper
• Yarrowia lipolytica lipase Lip2: an efficient enzyme for the production of concentrates of docosahexaenoic acid ethyl ester.

  2014cited by this paper
• Pichia pastoris: protein production host and model organism for biomedical research.

  2013cited by this paper
• Differential requirements for mRNA folding partially explain why highly expressed proteins evolve slowly

  2013cited by this paper
• de novo Design and Synthesis of Candida antarctica Lipase B Gene and α-Factor Leads to High-Level Expression in Pichia pastoris

  2013cited by this paper
• Constitutive Expression of Yarrowia lipolytica Lipase LIP2 in Pichia pastoris Using GAP as Promoter

  2012cited by this paper
• A Simple and Accurate Two-Step Long DNA Sequences Synthesis Strategy to Improve Heterologous Gene Expression in Pichia

  2012cited by this paper
• Strong association between mRNA folding strength and protein abundance in S. cerevisiae

  2012cited by this paper
• Parallel on-chip gene synthesis and application to optimization of protein expression

  2011cited by this paper
• Two-step synthesis of fatty acid ethyl ester from soybean oil catalyzed by Yarrowia lipolytica lipase

  2011cited by this paper
• Improving recombinant eukaryotic membrane protein yields in Pichia pastoris: The importance of codon optimization and clone selection

  2011cited by this paper
• Secretory expression and purification of a soluble NADH cytochrome b5 reductase enzyme from Mucorracemosus in Pichiapastoris based on codon usage adaptation

  2010cited by this paper
• Rationally engineered double substituted variants of Yarrowia lipolytica lipase with enhanced activity coupled with highly inverted enantioselectivity towards 2‐bromo phenyl acetic acid esters

  2010cited by this paper
• Transcription termination by nuclear RNA polymerases.

  2009cited by this paper
• Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter.

  2009cited by this paper
• Introduction: distinctions between Pichia pastoris and other expression systems.

  2007cited by this paper
• High-level expression of extracellular lipase Lip2 from Yarrowia lipolytica in Pichia pastoris and its purification and characterization.

  2007cited by this paper
• Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production

  2005cited by this paper
• Gene2Oligo: oligonucleotide design for in vitro gene synthesis

  2004cited by this paper
• Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter.

  2004cited by this paper
• Synonymous codon usage bias and the expression of human glucocerebrosidase in the methylotrophic yeast, Pichia pastoris.

  2002cited by this paper
• Autocloning and Amplification of LIP2 inYarrowia lipolytica

  2000cited by this paper
• A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris.

  1998cited by this paper
• Recent Advances in the Expression of Foreign Genes in Pichia pastoris

  1993cited by this paper
• Gene expression in yeast: Pichia pastoris.

  1991cited by this paper
• Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris

  1989cited by this paper

• Lipase: Recombinant Production Methods, Origins, and Industrial Uses

  2025cites this paper
• Recent advances in engineering microbial lipases for industrial applications.

  2025cites this paper
• Continuous cell recycling in methylotrophic yeast Pichia pastoris to enhance product yields: a case study with Yarrowia lipolytica lipase Lip2

  2024influential citation
• Medium optimization for enhanced production of recombinant lignin peroxidase in Pichia pastoris

  2022cites this paper
• Bakteriyel Kaynaklı Lipaz Geninin Pichia pastoris’te Klonlanması, Ekspresyonu ve Rekombinant Enzimin Saflaştırılması

  2022cites this paper
• Expression of Drosophila melanogaster acetylcholinesterase (DmAChE) gene splice variants in Pichia pastoris and evaluation of its sensitivity to organophosphorus pesticides

  2021cites this paper
• Pichia pastoris (Komagataella phaffii) as a Cost-Effective Tool for Vaccine Production for Low- and Middle-Income Countries (LMICs)

  2021cites this paper
• Increased mRNA Stability and Expression Level of Croceibacter atlanticus Lipase Gene Developed through Molecular Evolution Process

  2021cites this paper
• Expression of novel acidic lipase from Micrococcus luteus in Pichia pastoris and its application in transesterification

  2021cites this paper
• Improved Production of Xylanase in Pichia pastoris and Its Application in Xylose Production From Xylan

  2021cites this paper
• Phytoplankton pangenome reveals extensive prokaryotic horizontal gene transfer of diverse functions

  2020cites this paper
• Co-Expression of a Thermally Stable and Methanol-Resistant Lipase and Its Chaperone from Burkholderia cepacia G63 in Escherichia coli

  2020cites this paper
• The expression level of a recombinant lipase predicted in silico by different codon optimization algorithms

  2019cites this paper
• Expression of Zea mays transglutaminase in Pichia pastoris under different promoters and its impact on properties of acidified milk protein concentrate gel.

  2019cites this paper
• Biotechnological Application of Non-conventional Yeasts for Xylose Valorization

  2019cites this paper
• Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in Pichia pastoris and Evaluation of Antitumor Activity In Vivo

  2018cites this paper
• Agro-Industrial Residues and Microbial Enzymes

  2017cites this paper
• Strategies of codon optimization for high-level heterologous protein expression in microbial expression systems

  2017cites this paper
• Integrated analysis of individual codon contribution to protein biosynthesis reveals a new approach to improving the basis of rational gene design

  2017cites this paper
• Enhanced expression of lipase I from Galactomyces geotrichum by codon optimisation in Pichia pastoris.

  2017cites this paper
• Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: A review.

  2017cites this paper
• Expression and comparison of codon optimised Aspergillus tubingensis amylase variants in Saccharomyces cerevisiae

  2017cites this paper
• Regulating unfolded protein response activator HAC1p for production of thermostable raw-starch hydrolyzing α-amylase in Pichia pastoris

  2017cites this paper
• Genetic modification and optimization of endo-inulinase for the enzymatic production of oligofructose from inulin

  2016cites this paper
• High-level secretory expression of Aspergillus exo-inulinase and its use in the preparation of fructose syrup from inulin

  2016cites this paper
• Pichiapastoris Production of Tat-NGB and Its Neuroprotection on Rat Pheochromocytoma Cells

  2015cites this paper