Purification of Gekko Small Peptide Fraction and Its Effect of Inducing Apoptosis of EC 9706 Esophageal Cancer Cells by Inhibiting PI3K/Akt/GLUT1 Signaling Pathway

This study aimed to isolate and purify a cytotoxic extraction from Gekko japonicus, identify its components and determine its cytotoxic activity in vitro. We isolated and identified the most potent cytotoxic Gekko small peptide LH‐20‐15. The identification and analysis of peptide sequences of LH‐20‐15 were performed by de novo peptide sequencing, and two new peptides were found. LH‐20‐15 significantly inhibited the proliferation of human esophageal squamous carcinoma EC 9706 cells in a dose‐dependent manner. Furthermore, LH‐20‐15 induced apoptosis in esophageal cancer cells by activating the mitochondrial apoptotic pathway. Further research showed that LH‐20‐15 inhibited the PI3 K/Akt/GLUT1 signaling pathway. In conclusion, LH‐20‐15 from Gekko japonicus is a peptide mixture and may inhibit EC 9706 cell proliferation and induce apoptosis by activating the mitochondrial apoptotic pathway. It also regulates glucose metabolism by targeting the PI3 K/Akt/GLUT1 signaling pathway. These small peptides could be new sources of natural cytotoxic ingredients against esophageal cancer with potential drug values.

• Publication year

  2021

• Venue

  Chemistry and Biodiversity

• Publication date

  2021-02-03

• Fields of study

  Medicine

• Identifiers
  DOI 10.1002/cbdv.202000720PMID 33534194
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Glut 1 in Cancer Cells and the Inhibitory Action of Resveratrol as A Potential Therapeutic Strategy

  2019cited by this paper
• Molecular Carcinogenesis

  2019cited by this paper
• The Warburg Effect Is Associated With Tumor Aggressiveness in Testicular Germ Cell Tumors

  2019cited by this paper
• Endogenous peptide LYENRL prevents the activation of hypertrophic scar-derived fibroblasts by inhibiting the TGF-β1/Smad pathway.

  2019cited by this paper
• GLUT1 and TUBB4 in Glioblastoma Could be Efficacious Targets

  2019cited by this paper
• Mung Bean (Vigna radiata L.): Bioactive Polyphenols, Polysaccharides, Peptides, and Health Benefits

  2019cited by this paper
• DT-13 inhibited the proliferation of colorectal cancer via glycolytic metabolism and AMPK/mTOR signaling pathway.

  2019cited by this paper
• Protein phosphatase 2A mediates JS‐K‐induced apoptosis by affecting Bcl‐2 family proteins in human hepatocellular carcinoma HepG2 cells

  2018cited by this paper
• Ablation of caspase-1 protects against TBI-induced pyroptosis in vitro and in vivo

  2018cited by this paper
• Structural characterization and anticancer activity (MCF7 and MDA-MB-231) of polysaccharides fractionated from brown seaweed Sargassum wightii.

  2018cited by this paper
• Dexmedetomidine attenuates lung apoptosis induced by renal ischemia–reperfusion injury through α2AR/PI3K/Akt pathway

  2018cited by this paper
• Antineoplastic activity of isoliquiritigenin, a chalcone compound, in androgen‐independent human prostate cancer cells linked to G2/M cell cycle arrest and cell apoptosis

  2018cited by this paper
• Glucose transporters in cancer – from tumor cells to the tumor microenvironment

  2018cited by this paper
• Effects of intrinsic metal ions of lentinan with different molecular weights from Lentinus edodes on the antioxidant capacity and activity against proliferation of cancer cells.

  2018cited by this paper
• Protein phosphatase 2A activation mechanism contributes to JS-K induced caspase-dependent apoptosis in human hepatocellular carcinoma cells

  2018cited by this paper
• Unraveling the bioactivity of anticancer peptides as deduced from machine learning

  2018influential reference
• JS-K as a nitric oxide donor induces apoptosis via the ROS/Ca2+/caspase-mediated mitochondrial pathway in HepG2 cells.

  2018cited by this paper
• Structure-based design for binding peptides in anti-cancer therapy.

  2018cited by this paper
• Increased Serotonin Signaling Contributes to the Warburg Effect in Pancreatic Tumor Cells Under Metabolic Stress and Promotes Growth of Pancreatic Tumors in Mice.

  2017cited by this paper
• The Warburg Effect: How Does it Benefit Cancer Cells?

  2016cited by this paper
• A new mechanism of trastuzumab resistance in gastric cancer: MACC1 promotes the Warburg effect via activation of the PI3K/AKT signaling pathway

  2016cited by this paper
• The Warburg effect and drug resistance

  2016cited by this paper
• Atrazine induces endoplasmic reticulum stress‐mediated apoptosis of T lymphocytes via the caspase‐8‐dependent pathway

  2016cited by this paper
• Roles of HDAC2 and HDAC8 in Cardiac Remodeling in Renovascular Hypertensive Rats and the Effects of Valproic Acid Sodium

  2016cited by this paper
• Metabolic phenotype of bladder cancer.

  2016cited by this paper
• Gecko proteins induce the apoptosis of bladder cancer 5637 cells by inhibiting Akt and activating the intrinsic caspase cascade

  2015cited by this paper
• The Warburg effect in tumor progression: mitochondrial oxidative metabolism as an anti-metastasis mechanism.

  2015cited by this paper
• Effects of ephrinB2 gene siRNA on the biological behavior of human colorectal cancer cells.

  2015cited by this paper
• Gecko Crude Peptides Induce Apoptosis in Human Liver Carcinoma Cells In Vitro and Exert Antitumor Activity in a Mouse Ascites H22 Xenograft Model

  2012cited by this paper
• Anti-Migration Effects of Gekko Sulfated Glycopeptide on Human Hepatoma SMMC-7721 Cells

  2011cited by this paper
• Possible involvement of activation of P53/P21 and demethylation of RUNX 3 in the cytotoxicity against Lovo cells induced by 5-Aza-2'-deoxycytidine.

  2009cited by this paper
• Role of the plasma membrane ROS-generating NADPH oxidase in CD34+ progenitor cells preservation by hypoxia.

  2007cited by this paper
• Trichosanthin induced apoptosis in HL-60 cells via mitochondrial and endoplasmic reticulum stress signaling pathways.

  2007cited by this paper

• Animal-derived peptides from Traditional Chinese medicines: medicinal potential, mechanisms, and prospects.

  2025cites this paper
• Inhibition of protein glycation by vasodilatory β-blockers - In vitro studies and in silico analyses.

  2025cites this paper
• Targeting Glucose Transporter 1 (GLUT1) in Cancer: Molecular Mechanisms and Nanomedicine Applications

  2025cites this paper
• Unveiling Potent Bioactive Compounds and Anti-Angiogenic Pathways in Gekko swinhonis Guenther for Gastric Cancer Therapy.

  2024cites this paper
• Saikosaponin-b2 Regulates the Proliferation and Apoptosis of Liver Cancer Cells by Targeting the MACC1/c-Met/Akt Signalling Pathway

  2024cites this paper
• Targeting the Glycolytic Enzyme PGK1 to Inhibit the Warburg Effect: A New Strategy for Keloid Therapy

  2023cites this paper
• Unraveling the Complexity of Regulated Cell Death in Esophageal Cancer: from Underlying Mechanisms to Targeted Therapeutics

  2023cites this paper