Effects of long-term fertilization on calcium-associated soil organic carbon: Implications for C sequestration in agricultural soils.

Although the contribution of calcium ion (Ca2+) to stabilizing organic carbon (OC) in soils has been known for years, we still have a limited understanding of the quantity and molecular composition of Ca2+ bound SOC (Ca-OC) evolution in response to long-term fertilization. Here we report the role of Ca2+ in the accumulation of OC in the topsoil (0-20 cm) from two long-term (25-37 years) fertilization experiment sites. Approximately 4.54-19.27% and 9.00-25.15% of SOC was bound with Ca2+ in the Ferric Acrisol and Fluvic Cambisol, respectively. The application of NPK mineral fertilizers (NPK) decreased (p < 0.05) the Ca-OC stocks from 3.40 t ha-1 to 0.96 t ha-1 and from 2.03 t ha-1 to 1.17 t ha-1 in the Ferric Acrisol and Fluvic Cambisol, respectively. Swine manure (M) addition did not change (p > 0.05) the Ca-OC stock in Ferric Acrisol, but enhanced (p < 0.05) that from 2.03 t ha-1 to 9.75 t ha-1 in Fluvic Cambisol. Fourier transform infrared and carbon (1s)-near X-ray absorption spectroscopies showed that Ca2+ was mainly bound with aromatic carbon and carboxylic carbon. Long-term M fertilization facilitated the binding of Ca2+ with O-alkyl C, suggesting an increment of Ca-linked polysaccharide. Calcium ion was preferentially associated with 13C enriched organic matter (OM). Mineral fertilization promoted the 13C-enriched organic compounds in the Ca-OC, while organic fertilization facilitated the binding of 13C-depleted organic C with Ca2+. This study suggests that Ca-OC may be a potentially vital and stable OC pool in arable soils, and provides direct evidence for the preferential association of OC with Ca2+ in edaphic environments.

• Publication year

  2021

• Venue

  Science of the Total Environment

• Publication date

  2021-02-02

• Fields of study

  Agricultural and Food Sciences, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.scitotenv.2021.145037PMID 33578172
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Ten-year long-term organic fertilization enhances carbon sequestration and calcium-mediated stabilization of aggregate-associated organic carbon in a reclaimed Cambisol

  2019cited by this paper
• Soil organic carbon storage as a key function of soils - A review of drivers and indicators at various scales

  2019cited by this paper
• Iron oxides selectively stabilize plant‐derived polysaccharides and aliphatic compounds in agricultural soils

  2019cited by this paper
• Arsenite and arsenate binding to ferrihydrite organo-mineral coprecipitate: Implications for arsenic mobility and fate in natural environments.

  2019cited by this paper
• Effect of land use and carbonates on organic matter stabilization and microbial communities in Mediterranean soils

  2019cited by this paper
• Organic matter facilitates the binding of Pb to iron oxides in a subtropical contaminated soil

  2018cited by this paper
• Long-term fertilization increases soil organic carbon and alters its chemical composition in three wheat-maize cropping sites across central and south China

  2018cited by this paper
• Soil organic carbon stocks controlled by lithology and soil depth in a Peruvian alpine grassland of the Andes

  2018cited by this paper
• Spatial Associations and Chemical Composition of Organic Carbon Sequestered in Fe, Ca, and Organic Carbon Ternary Systems.

  2018cited by this paper
• The synergistic effect of calcium on organic carbon sequestration to ferrihydrite

  2018cited by this paper
• Climate-driven thresholds in reactive mineral retention of soil carbon at the global scale

  2018cited by this paper
• Distribution and thermal stability of physically and chemically protected organic matter fractions in soils across different ecosystems

  2018cited by this paper
• Beyond clay: towards an improved set of variables for predicting soil organic matter content

  2018cited by this paper
• Organic carbon characteristics in density fractions of soils with contrasting mineralogies

  2017cited by this paper
• Soil Ca alters processes contributing to C and N retention in the Oa/A horizon of a northern hardwood forest

  2017cited by this paper
• Mineral Availability as a Key Regulator of Soil Carbon Storage.

  2017cited by this paper
• Response of soil organic matter fractions and composition of microbial community to long-term organic and mineral fertilization

  2017cited by this paper
• Dynamics of soil organic carbon and nitrogen following agricultural abandonment in a karst region

  2017cited by this paper
• Changes in organic carbon content and its physical and chemical distribution in paddy soils cultivated under different fertilisation practices

  2017cited by this paper
• Calcium-mediated stabilisation of soil organic carbon

  2017cited by this paper
• Iron-Bound Organic Carbon in Forest Soils: Quantification and Characterization

  2016cited by this paper
• Little effects on soil organic matter chemistry of density fractions after seven years of forest soil warming

  2016cited by this paper
• Some concepts of soil organic carbon characteristics and mineral interaction from a review of literature

  2016cited by this paper
• Chapter One - Mineral–Organic Associations: Formation, Properties, and Relevance in Soil Environments

  2015cited by this paper
• Formation of soil organic matter via biochemical and physical pathways of litter mass loss

  2015cited by this paper
• The Performance of Visible, Near-, and Mid-Infrared Reflectance Spectroscopy for Prediction of Soil Physical, Chemical, and Biological Properties

  2014cited by this paper
• Soil Chemical Insights Provided through Vibrational Spectroscopy

  2014cited by this paper
• Physical and chemical stabilization of soil organic carbon along a 500-year cultived soil chronosequence originating from estuarine wetlands: Temporal patterns and land use effects

  2014cited by this paper
• Translocation of metals by trees and fungi regulates pH, soil organic matter turnover and nitrogen availability in acidic forest soils

  2013cited by this paper
• Potential influence of poorly crystalline minerals on soil chemistry in Podzols of southwestern Canada

  2013cited by this paper
• Characterization of Humic Carbon in Soil Aggregates in a Long-term Experiment with Manure and Mineral Fertilization

  2012cited by this paper
• Effects of pH and calcium on soil organic matter dynamics in Alaskan tundra

  2012cited by this paper
• Mid-infrared spectral interpretation of soils: Is it practical or accurate?

  2012cited by this paper
• C 1s K-edge near edge X-ray absorption fine structure (NEXAFS) spectroscopy for characterizing functional group chemistry of black carbon

  2011cited by this paper
• Persistence of soil organic matter as an ecosystem property

  2011cited by this paper
• Chemical Differences in Soil Organic Matter Fractions Determined by Diffuse‐Reflectance Mid‐Infrared Spectroscopy

  2011cited by this paper
• Temperature and soil organic matter decomposition rates – synthesis of current knowledge and a way forward

  2011cited by this paper
• Soil aggregation and organic carbon fractions affected by long-term fertilization in a red soil of subtropical China

  2010cited by this paper
• FT-IR as an alternative method for measuring chemical properties during composting.

  2010cited by this paper
• Crop residue removal and fertilizer N: Effects on soil organic carbon in a long-term crop rotation experiment on a Udic Boroll

  2010cited by this paper
• Crop yield and soil responses to long-term fertilization on a red soil in southern China.

  2009cited by this paper
• Long-term impact of fertilizers on soil organic carbon pools and sequestration rates in maize–wheat–cowpea cropping system

  2008cited by this paper
• Organo-mineral associations in temperate soils: Integrating biology, mineralogy, and organic matter chemistry

  2008cited by this paper
• Sorptive stabilization of organic matter in soils by hydrous iron oxides

  2007cited by this paper
• Carbon and nitrogen mineralization in acidic, limed and calcareous agricultural soils : Apparent and actual effects

  2007cited by this paper
• Stabilization of Soil Organic Matter: Association with Minerals or Chemical Recalcitrance?

  2006cited by this paper
• Stabilization of organic matter in temperate soils: mechanisms and their relevance under different soil conditions – a review

  2006cited by this paper
• Compound-specific stable-isotope (δ13C) analysis in soil science

  2005cited by this paper
• Poorly crystalline mineral phases protect organic matter in acid subsoil horizons

  2005cited by this paper
• Carbon K‐Edge NEXAFS and FTIR‐ATR Spectroscopic Investigation of Organic Carbon Speciation in Soils

  2005cited by this paper
• Soil Carbon Sequestration Impacts on Global Climate Change and Food Security

  2004cited by this paper
• Humic acids in the first generation of EUROSOILS

  2003cited by this paper
• FTIR techniques in clay mineral studies

  2003cited by this paper
• Measuring and understanding carbon storage in afforested soils by physical fractionation

  2002cited by this paper
• Acid fumigation of soils to remove carbonates prior to total organic carbon or CARBON‐13 isotopic analysis

  2001cited by this paper
• Physical and chemical protection of soil organic carbon in three agricultural soils with different contents of calcium carbonate

  2000cited by this paper
• The role of DOM sorption to mineral surfaces in the preservation of organic matter in soils.

  2000cited by this paper
• Effect of Ca on the solubility and molecular size distribution of DOC and Cu binding in soil solution samples

  1998cited by this paper
• The biogeochemistry of calcium at Hubbard Brook

  1998cited by this paper
• Fractionation of dissolved organic matter affected by polyvalent metal cations

  1998cited by this paper
• Total carbon and nitrogen in the soils of the world

  1996cited by this paper
• Dissolution and Fractionation of Calcium—Bound and Iron—and Aluminum—Bound Humus in Soils

  1993cited by this paper
• Stabilité biologique et comportement physique d'un complexe argilo-humique placé dans différentes conditions de saturation en calcium ou en potassium

  1984cited by this paper

• High-Dose Biochar Hinders Micro/Nanoplastic-Induced Soil Positive Priming by Reducing Substrate Quality and Microbial Activity.

  2026cites this paper
• Impacts of Spartina alterniflora invasion on fractions and fungal communities of mineral-associated organic carbon in subtropical coastal wetlands of China

  2026cites this paper
• Conservation tillage and wheat straw managements improve soil organic carbon sequestration via calcium-mediated microbial communities and aggregate stability in Calcaric Cambisols.

  2025cites this paper
• Advances in Understanding Carbon Storage and Stabilization in Temperate Agricultural Soils

  2025cites this paper
• Conservation agriculture enhances ecosystem services and sustainability of the system over conventional agriculture

  2025cites this paper
• Conservation Tillage Increases Carbon Storage by Regulating Mineral‐Mediated Aggregate Stability and Carbon Chemistry

  2025cites this paper
• Soil Carbonate Dominates Calcium‐Bound Organic Carbon Storage at the Continental Scale

  2025cites this paper
• Long-term organic fertilization reduces cadmium bioavailability via enhanced binding to organic fractions and Fe-bearing minerals.

  2025cites this paper
• Inorganic amendments increase soil carbon sequestration across global acidic agroecosystems: A meta-analysis

  2025cites this paper
• Calcium peroxide capping stabilizes organic carbon in anoxic sediments via mineral protection.

  2025cites this paper
• Long-term nitrogen addition enhanced soil carbon sequestration through coupled physicochemical and microbial mechanisms in a temperate forest.

  2025cites this paper
• Beneficial Effects of Fulvic Acid on Activating Phosphorus and Stimulating phoD Bacteria Were Greatly Weakened by Soil Drying in Calcareous Soils.

  2025cites this paper
• Liming mitigates earthworm-induced CO2 and N2O emissions from a sugar maple (Acer saccharum Marsh.) forest soil

  2025cites this paper
• Accumulation mechanism of iron-bound organic carbon in soil amended with straw and biochar

  2025cites this paper
• Nitrogen-induced soil acidification mitigates the negative effects of nitrogen addition on SOC stability

  2025cites this paper
• Microbial Necromass Carbon Distribution Differs Between Four Soil Types After Long‐Term Straw Return

  2025influential citation
• Study on the Use of Soda Saline–Alkali Soil as a Rice-Seedling-Raising Soil After Short-Term Improvement

  2025cites this paper
• Fertilization effects on soil organic matter chemistry

  2025cites this paper
• Contrasting contributions of microbial and plant-derived C to soil carbon in desertified grassland restoration

  2025cites this paper
• Linkages Between Exchangeable Cations, Maize Growth and Yield After Prescribed Fire and Amendment Interaction in a Tropical Environment

  2025cites this paper
• Soil organic matter characteristics of four soil types under different conservation strategies across Hubei Province

  2024influential citation
• The impact of non-grain conversion of cultivated land on the relationship between agricultural carbon supply and demand

  2024cites this paper
• Long‐term nitrogen and phosphorus addition have stronger negative effects on microbial residual carbon in subsoils than topsoils in subtropical forests

  2024influential citation
• Divergent controls of exchangeable calcium and iron oxides in regulating soil organic carbon content across climatic gradients in arid regions

  2024cites this paper
• Variations in the quantity and chemical composition of soil dissolved organic matter along a chronosequence of wolfberry plantations in an arid area of Northwest China

  2024cites this paper
• How does phosphorus fertilizer improve the stability of soil aggregates? Evidence from a decade fertilization experiment

  2024cites this paper
• Soil inorganic carbon storage and spatial distribution in irrigated farmland on the North China Plain

  2024cites this paper
• Impact of mineral and organic fertilisation practices on elemental authenticity signature on apple Royal Gala from protected geographical indication (PGI) “Maçã de Alcobaça”

  2024cites this paper
• Impact of the Combination of Chemical and Organic Fertilization on the Growth and Yield of Pineapple under Two Shade Net Conditions

  2024cites this paper
• Saline-alkali land reclamation boosts topsoil carbon storage by preferentially accumulating plant-derived carbon.

  2024cites this paper
• Soil Quality Testing and Counseling Coastal Agricultural Communities in Taiwan

  2024influential citation
• Variable responses of mineral-bound soil organic carbon to land cover change in southern China’s coastal wetlands

  2024cites this paper
• Joint regulation of the soil organic carbon accumulation by mineral protection and microbial properties following conservation practices

  2024cites this paper
• Drivers of carbon stabilization and sequestration in Brazil’s black soils

  2024cites this paper
• Effect of 9-year water and nitrogen additions on microbial necromass carbon content at different soil depths and its main influencing factors.

  2024cites this paper
• Responses of various organic carbon pools to elevated temperatures in soils.

  2024cites this paper
• Anthropic dark soils horizons in western Siberian taiga: origin, soil chemistry and sustainability of organic matter

  2024cites this paper
• Soil phosphorus compared to nitrogen limitation increases the uncertainty of subsoil organic carbon sequestration in Pinus massoniana mixed forests.

  2024cites this paper
• Calcium forms influence soil organic carbon by mediating labile organic carbon fractions, carbon pool management indices and microbial communities in calcareous alkaline soils

  2024cites this paper
• Root architectures differentiate the composition of organic carbon in bauxite residue during natural vegetation.

  2023cites this paper
• Nitrogen increases soil organic carbon accrual and alters its functionality

  2023cites this paper
• Straw return plus zinc fertilization increased the accumulations and changed the chemical compositions of mineral-associated soil organic carbon

  2023cites this paper
• Impact of Rubber-Based Land Use Changes on Soil Properties and Carbon Pools: A Meta-Analysis

  2023cites this paper
• Wollastonite addition stimulates soil organic carbon mineralization: Evidences from 12 land-use types in subtropical China

  2023cites this paper
• Characteristics of Soil Calcium Content Distribution in Karst Dry-Hot Valley and Its Influencing Factors

  2023cites this paper
• Carbon Sequestration in Remediated Post-Mining Soils: A New Indicator for the Vertical Soil Organic Carbon Variability Evaluation in Remediated Post-Mining Soils

  2023cites this paper
• Assessing land use type impacts on soil quality: Application of multivariate statistical and expert opinion-followed indicator screening approaches

  2023cites this paper
• Contrasting priming effect intensities and drivers in single and repeated glucose additions to a forest soil receiving long-term N fertilization

  2023cites this paper
• The Different Factors Driving SOC Stability under Different N Addition Durations in a Phyllostachys edulis Forest

  2023cites this paper
• Calcium promotes persistent soil organic matter by altering microbial transformation of plant litter

  2023cites this paper
• Agave durangensis vinasse as a biocide for forest pest control

  2022cites this paper
• Effects of experimental nitrogen deposition on soil organic carbon storage in Southern California drylands

  2022cites this paper
• Nutrient addition affects stability of soil organic matter and aggregate by altering chemical composition and exchangeable cations in desert steppe in northern China

  2022cites this paper
• Insight on efficiently oriented oxidation of petroleum hydrocarbons by redistribution of oxidant through inactivation of soil organic matter coupled with passivation of manganese minerals.

  2022cites this paper
• Does the Application of ICTs Improve the Efficiency of Agricultural Carbon Reduction? Evidence from Broadband Adoption in Rural China

  2022cites this paper
• Microbial community mediates hydroxyl radical production in soil slurries by iron redox transformation.

  2022cites this paper
• Soil organic carbon accrual due to more efficient microbial utilization of plant inputs at greater long-term soil moisture

  2022cites this paper
• Visualizing Mineral-Associated Organic Matters in Long-Term Fertilization Treated Soils by NanoSIMS and SR-FTIR

  2022cites this paper
• Soil carbon supplementation: Improvement of root-surrounding soil bacterial communities, sugar and starch content in tobacco (N. tabacum).

  2021cites this paper
• Warming and humidification mediated changes of DOM composition in an Alfisol.

  2021cites this paper
• Microbial pathways account for the pH effect on soil N2O production

  2021cites this paper
• Higher Long-Term Soil Moisture Increases Organic Carbon Accrual Through Microbial Conversion of Organic Inputs

  2021cites this paper
• Stover return and nitrogen application affect soil organic carbon and nitrogen in a double-season maize field.

  2021cites this paper
• Soil organic carbon accrual due to more efficient microbial utilization of plant inputs at greater long-term soil moisture

  year unknowncites this paper
• Long-term calcium fertilization mediates microbial and plant-derived carbon accumulation by influencing iron oxides in red soils

  year unknowncites this paper